Instructions for Submitting Diagnostic Specimens to the DVBD Arbovirus Diagnostic Laboratory
CDC submission form
Please read these instructions for sending diagnostic specimens to CDC. Complete a CDC submission form (CDC 50.34) CDC Data and Specimen Handling (DASH) section form 50.34 for submission of laboratory specimens or submit through CSTOR.
NOTE: Testing will not be initiated without the inclusion of:
- Date of onset of symptoms
- Date of specimen collection
NOTE: If the specimen collection occurs within 8 days after the onset of symptoms, a convalescent specimen may be requested.
- Any pertinent travel history (3 months prior to the date of symptom
- The patient’s name and/or unique ID and DOB and sex (REQUIRED for submitting specimens)
To enable printing of CDC submission form 50.34, each of the following fields must be completed, as directed:
- Specimen Origin field (located on 1st page, top left corner), select “HUMAN” from the drop-down menu
- Test Order Name field (located on 1st page, top left), select “ARBOVIRUS SEROLOGY” from the drop-down menu
- Original Submitter e-mail field (located on 1st page, middle right box), type your e-mail address
- Brief clinical summary (located on second page, top of page), include the name(s) of the arbovirus(es) for which you are requesting testing, if known. Also, if you would like to request testing other than serology, please note the type of test requested in this field.
For information regarding shipping packages and applicable regulations, please refer to the NCEZID Division of Scientific Resources’ Specimen Management site for specific information.
Specimen types and amounts
- Acute and convalescent specimens, if available, should be sent together.
- Ideal timing of specimens for serology:
Ideal timing of specimens for serology Specimen Timing Acute 3 to 10 days after onset of symptoms Convalescent 2-3 weeks after acute sample
- At least 0.5 mL of serum and/or 1.0 mL of CSF is required for serology testing. CSF specimens are routinely tested undiluted and therefore require larger amounts. Whole blood will not be accepted for serology testing. Please transfer serum or CSF to a plastic tube with screw cap measuring no more than 5 cm tall and approximately 13 mm in diameter (e.g., 1.8 mL cryotube or 2.0 mL microtube).
- For serology or nucleic acid testing, the specimen should be kept cold or frozen. The sample may be placed in an insulated container with blue ice packs. Additional blue ice packs should be used in the summer to ensure specimen integrity in hot weather.
- For nucleic acid amplification testing, acceptable specimens are serum or cerebrospinal fluid (CSF). Formalin-fixed specimens are not tested at DVBD and can be submitted to the Special Pathogens Laboratory in Atlanta, GA for immunohistochemistry:
Infectious Disease Pathology Branch
Centers for Disease Control and Prevention (MS-G32)
1600 Clifton Rd, NE
Atlanta, GA 30333
Given the current volume of samples, test results will not be available for at least 3 weeks after specimen receipt. Reporting times for test results may be longer during summer months or when arbovirus activity increases. Initial serological testing will be performed using IgM capture ELISA and/or IgM Microsphere Immunoassay (MIA) (as appropriate for clinical presentation). If the initial results are positive, further confirmatory testing may delay the reporting of final results. ALL RESULTS WILL BE SENT TO THE APPROPRIATE STATE HEALTH DEPARTMENT. All specimens should be submitted to CDC through state health departments and NOT submitted directly to CDC.
Send all specimens to:
ATTN: Arbovirus Diagnostic Laboratory
3156 Rampart Road
Fort Collins, CO 80521