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Table 1 version 5. Submission of Enteric Disease-associated Bacterial Isolates and Specimens

Table 1 version 5 PDF [4 pages]. Submission of enteric disease-associated bacterial isolates and specimens to the Enteric Diseases Laboratory Branch, effective as of February 6, 2018. Please send the following isolates or specimens for the following services to the attention of the appropriate contact person to Centers for Disease Control and Prevention; Data & Specimen Handling Section (DASH); 1600 Clifton Road, NE; Atlanta, GA 30329-4027. Please include the Laboratory Contact Person in the attention line.  Test codes for laboratory examination requested.

Table 1 version 5. Submission of Enteric Disease bacterial Isolates (Specimens)
Specimen Service Priority Number to send, Submission scheme Laboratory Examination Requested (test code, if applicable) Contact Person§ Frequency/timing Accompanying Document
Clinical, food, environmental specimens for botulism investigations Outbreak investigation ASAP^ All collected specimens Botulism laboratory confirmation (CDC-10132) Carolina Luquez or   Janet Dykes As soon as specimens collected CDC form 50.34
Fecal, environmental and food specimens for Bacillus cereus, Clostridium perfringens and Staphylococcus aureus foodborne investigation Outbreak investigation ASAP^ consult contact person for number to send Detection – foodborne outbreak (CDC-10104, B. cereus ), (CDC-10111, C. perfringens ) or (CDC- 10113, S. aureus ) Carolina Luquez or   Gerry Gomez as needed CDC form 50.34
Clinical, food, and environmental specimens for the isolation of Campylobacter, Cronobacter, Listeria, Salmonella, Shigella , STEC and other diarrheagenic E. coli ^^, Vibrio, Yersinia (non-pestis) and other pathogenic Enterbacteriaceae Outbreak/case investigation ASAP^  consult contact person for number to send Enteric Isolation (CDC-10106) Haley Martin or    Nancy Strockbine as needed CDC form 50.34
Serum specimens: from suspect cases of Shiga-toxin producing E. coli , Campylobacter, Vibrio cholerae and Salmonella serotype Typhi  Outbreak
investigation/surveillance
 As scheduled consult contact person for type of specimen to send  Enteric serology: CDC- 10452 (E. coli); CDC- 10455 (Campylobacter); CDC-10454 (V. cholerae ); or CDC- 10453 (Salmonella serotype Typhi) Rachael Aubert or   Patti Fields consult contact person for frequency/timing CDC form 50.34
STEC (Shiga toxin-producing E. coli) broths Reference/surveillance ASAP^ Any broths positive by Shiga toxin testing but culture-negative for STEC but which produce growth on subculture Toxin typing, STEC isolation from enrichment broth (10105) Nancy Strockbine or Devon Stripling or   Haley Martin as needed  CDC form 50.34
Table 1 version 5. Submission of Enteric Disease Bacterial Isolates (Organism)
Organism Service Priority Number to send, Submission scheme Laboratory Examination Requested (test code, if applicable) Contact Person§ Frequency/timing Accompanying Document
Aeromonas and related organisms Reference/surveillance At submitter’s convenience any isolates you would like to have analyzed Identification, subtyping (CDC-10120) Cheryl Tarr or      Monica Santovenia as needed CDC form 50.34
Bacillus cereus Outbreak investigation ASAP^ consult contact person for number to send Detection – foodborne outbreak (CDC-10104) Carolina Luquez or   Gerry Gomez as needed CDC form 50.34
Campylobacter Outbreak investigation ASAP^ consult contact person for number to send Campylobacter subtyping (CDC-10127) Janet Pruckler or Rachael Aubert every suspected outbreak CDC form 50.34
Campylobacter  (FoodNet Sites Only) NARMSC As scheduled Every 2nd, 3rd, 5th, or all isolates (varies by state) susceptibility testing for NARMS surveillance Hayat Caidi or Jason Folster at least quarterly NARMS logsheet
Campylobacter, Helicobacter, and related organisms Reference/surveillance At submitter’s convenience isolates that cannot be identified or atypical / rare isolates Identification (CDC-10126) Janet Pruckler or Rachael Aubert as needed CDC form 50.34
Clostridium botulinum and other botulinum producing species of Clostridia Reference/surveillance ASAP^ consult contact person for number to send Botulinum toxin productin Clostridia subtyping
(CDC-10133)
Janet Dykes or Carolina Luquez as needed CDC form 50.34.
DSAT approved
Form 2 transfer
Clostridium perfringens Outbreak investigation ASAP^ consult contact person for number to send Detection – foodborne outbreak (CDC-10111) Carolina Luquez or   Gerry Gomez As needed CDC form 50.34
Cronobacter sakazakii Outbreak investigation ASAP^ Every isolate CDC-10123 Cheryl Tarr Every suspected case CDC form 50.34
Cronobacter species Reference/surveillance At submitter’s convenience, or ASAP^ if C.sakazakii has not been ruled out Any isolates requiring speciation CDC-10123 Cheryl Tarr As needed CDC form 50.34
Enterobacteriaceae , other Reference/surveillance At submitter’s convenience any isolates implicated in enteric disease Identification, serotyping, subtyping (CDC-10123) Cheryl Tarr As needed CDC form 50.34
Escherichia coli, diarrheagenic^^ Outbreak investigation ASAP^ 3 representative isolates Susceptibility testing (CDC-10116); other testing upon request Nancy Strockbine or Devon Stripling or  Haley Martin In every suspected outbreak except multistate outbreak, in which case CDC will request CDC form 50.34
Escherichia coli O157 NARMSC As scheduled every 20th** Susceptibility testing for NARMS surveillance Hayat Caidi or Jason Folster at least quarterly NARMS logsheet
Escherichia Reference/surveillance At submitter’s convenience atypical isolates, rare serovars, or isolates that cannot be identified Identification, serotyping, subtyping, virulence profiling (CDC-10114) Nancy Strockbine or Devon Stripling or Haley Martin As needed CDC form 50.34
Listeria monocytogenes  Outbreak investigation ASAP^* every isolate from patients and implicated foods Identification, serotyping, subtyping (CDC-10129) Cheryl Tarr or      Zuzana Kucerova every time it is isolated/as soon as it is isolated CDC form 50.34
Listeria, species other than monocytogenes Reference/surveillance ASAP^  any isolates obtained from a normally sterile body site  Identification, serotyping, subtyping (CDC-10129) Cheryl Tarr or Zuzana Kucerova every time it is isolated/as soon as it is isolated  CDC form 50.34
Salmonella  Reference/surveillance  At submitter’s convenience  atypical isolates, rare serovars, or
isolates that cannot be identified
 Salmonella identification and serotyping (CDC-10110)  Susan Van Duyne or Ana Lauer  as needed  CDC form 50.34
Salmonella (all non-typhoidal serotypes and serotype Paratyphi B) NARMSC As scheduled every 20th** susceptibility testing for NARMS surveillance Hayat Caidi or Jason Folster at least quarterly NARMS logsheet
Salmonella (all non-Typhi serotypes) Outbreak investigation ASAP^ 3 representative isolates Salmonella subtyping (CDC-10108) Susan Van Duyne or Ana Lauer Please send in every suspected outbreak unless multistate, in which case CDC will request CDC form 50.34
Salmonella (typhoidal serotypes Paratyphi A, Paratyphi C and Typhi) NARMSC As scheduled ALL susceptibility testing for NARMS surveillance Hayat Caidi or Jason Folster at least quarterly NARMS logsheet
Salmonella serotype Typhi Outbreak investigation ASAP^ 3 representative
isolates
Salmonella subtyping (CDC-10108) Susan Van Duyne or Ana Lauer In every suspected outbreak except multistate outbreak, in which case CDC will request CDC form 50.34
Shigella Reference/surveillance At submitter’s convenience any isolates you would like to have analyzed Identification, subtyping (CDC-10114) Nancy Strockbine or Devon Stripling or Haley Martin as needed CDC form 50.34
Shigella Outbreak investigation ASAP^ 3 representative
isolates
Susceptibility testing (CDC-10116) Nancy Strockbine or Devon Stripling or Haley Martin In every suspected outbreak except multistate outbreak, in which case CDC will request CDC form 50.34
Shigella NARMSC As scheduled every 20th** susceptibility testing for NARMS surveillance Hayat Caidi or Jason Folster  at least quarterly  NARMS logsheet
Staphylococcus aureus Outbreak investigation  ASAP^  consult contact person for number to send  Detection – foodborne outbreak (CDC-10113) Carolina Luquez or Gerry Gomez  as needed  CDC form 50.34
 Vibrio cholerae  Reference/surveillance  ASAP^  every isolate  Identification, virulence testing (10119)  Cheryl Tarr or Monica Santovenia  every time it is isolated/as soon as it is isolated CDC form 50.34
Vibrio mimicus Reference/surveillance At submitter’s convenience every isolate Identification, virulence testing (CDC-10120) Cheryl Tarr or Monica Santovenia at least quarterly CDC form 50.34
Vibrio, non-cholerae Outbreak investigation ASAP^ All isolates Identification, subtyping (CDC-10122) Cheryl Tarr or Monica Santovenia every suspected outbreak CDC form 50.34
Vibrio, non-cholerae NARMSC As scheduled ALL susceptibility testing for NARMS surveillance Hayat Caidi or Jason Folster at least quarterly NARMS logsheet
Vibrio and related organisms Reference/surveillance At submitter’s convenience any isolates requiring speciation or that you would like to have analyzed Identification, subtyping (CDC-10120) Cheryl Tarr or Monica Santovenia as needed CDC form 50.34
Yersinia (non-pestis ) Reference/surveillance At submitter’s convenience any isolates you would like to have analyzed Identification, serotyping, biotyping, subtyping
(CDC-10123)
Cheryl Tarr as needed CDC form 50.34

^Isolates/specimens with a priority of “ASAP” should be shipped as soon as possible (within one business day of presumptive identification)
^^Escherichia coli , diarrheagenic = Shiga toxin-producing E. coli (STEC), Enterotoxigenic E. coli (ETEC), Enteropathogenic E. coli (EPEC), Enteroinvasive E. coli (EIEC) and Enteroaggregative E. coli ( EAEC)
CNational Antimicrobial Resistance Monitoring System
*Laboratories that are performing whole genome sequencing and submitting data in real time for Listeria can ship isolates quarterly instead of ASAP
**For non-typhoidal Salmonella, Shigella , and E. coli O157 isolates included in NARMS surveillance, the 1st isolate collected each year should be submitted, followed by every 20th isolate
§email addresses and phone numbers for contact persons are shown below:
General inquiries: entericbacteria@cdc.gov, 404-639-3334
Rachael Aubert: vrl7@cdc.gov, 404-639-3816
Hayat Caidi: foi0@cdc.gov, 404-639-0766
Janet Dykes: jkd1@cdc.gov, 404-639-3625
Patti Fields: pif1@cdc.gov, 404-639-1748
Jason Folster: gux8@cdc.gov, 404-639-4948
Gerry Gomez: goe4@cdc.gov, 404-639-0537
Zuzana Kucerova: zik0@cdc.gov, 404-718-4143
Ana Lauer: ybp6@cdc.gov, 404-639-2117
Carolina Luquez: fry6@cdc.gov, 404-639-0896
Haley Martin: hvw0@cdc.gov, 404-639-1612
Janet Pruckler: jmp5@cdc.gov, 404-639-4770
Monica Santovenia: ixi9@cdc.gov, 404-718-1446
Devon Stripling: euo4@cdc.gov, 404-639-2251
Nancy Strockbine: nas6@cdc.gov, 404-639-4186
Cheryl Tarr: crt6@cdc.gov, 404-639-2011
Susan Van Duyne: mdv9@cdc.gov, 404-639-0186

Public health officials (at the state and territorial public health laboratories and at CDC) can communicate about pending and current isolate requests via email
(entericbacteria@cdc.gov), a phone call to the Enteric Diseases Laboratory Branch at 404-639-3334, or by contacting specific personnel listed in Table 1 version 5.

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