Table 1 version 6. Submission of Enteric Disease-associated Bacterial Isolates and Specimens

Table 1 version 6 PDF [4 pages]. Submission of enteric disease-associated bacterial isolates and specimens to the Enteric Diseases Laboratory Branch, effective as of September 1, 2022 (memo [PDF – 3 pages]). Please send isolates or specimens for the following services to the attention of the appropriate contact person at the Centers for Disease Control and Prevention. Addresses and points of contact can be found at the links in the table under “Preparation and Shipping Instructions.” Please include the Laboratory Contact Person in the attention line.

Table 1 version 6. Submission of Enteric Disease bacterial Isolates (Specimens)
Specimen Service Priority Frequency/timing Number to send, Submission scheme Laboratory Examination Requested (test code, if applicable) Preparation and Shipping Instructions§
Clinical, food, environmental specimens for botulism investigations Outbreak investigation ASAP^ As soon as specimens collected All collected specimens Botulism laboratory confirmation (CDC-10132) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Fecal, environmental and food specimens for Bacillus cereus foodborne investigation Outbreak investigation ASAP^ as needed consult contact person for number to send Bacillus cereus Detection – foodborne outbreak (CDC-10104) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Fecal, environmental and food specimens for Clostridium perfringens foodborne investigation Outbreak/case investigation ASAP^ as needed consult contact person for number to send Clostridium perfringens Detection – foodborne outbreak (CDC-10111) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Fecal, environmental and
food specimens for
Staphylococcus aureus
foodborne investigation
Outbreak investigation ASAP^ as needed consult contact person for number to send Staphylococcus aureus Detection – foodborne outbreak (CDC-10113) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Clinical, food, and environmental specimens for the isolation of Campylobacter, Cronobacter, Listeria, Salmonella, Shigella, STEC and other diarrheagenic E. coli ^^, Vibrio, Yersinia (non-pestis) and other pathogenic Enterbacteriaceae Outbreak/case investigation ASAP^ as needed consult contact person for number to send Enteric Isolation (CDC-10106) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Serum specimens: from suspect cases of Campylobacter Outbreak investigation/surveillance As scheduled consult contact person for frequency/timing consult contact person for type of specimen to send Campylobacter species serology (10455) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Serum specimens: from suspect cases of Shiga-toxin producing E. coli Outbreak investigation/surveillance As scheduled consult contact person for frequency/timing consult contact person for type of specimen to send Escherichia coli (STEC) serology (not serotyping): CDC-10452 (E. coli) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Serum specimens: from suspect cases of Salmonella serotype Typhi Outbreak investigation/surveillance As scheduled consult contact person for frequency/timing consult contact person for type of specimen to send Salmonella serotype Typhi (only) serology (10453) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Serum specimens: from suspect cases of Vibrio cholerae Outbreak investigation/surveillance As scheduled consult contact person for frequency/timing consult contact person for type of specimen to send Vibrio cholerae serology (10454) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
STEC (Shiga toxin-producing E. coli) broths Reference/surveillance ASAP^ as needed Broths from cases of high public health importance (e.g., severe or unusual disease, outbreak-associated or unusual epidemiology) that are positive by Shiga toxin testing but culture-negative for STEC and produce growth on subculture. Consult contact person for prior approval. Toxin typing, STEC isolation from enrichment broth (10105) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Table 1 version 6. Submission of Enteric Disease Bacterial Isolates (Organisms)
Organism Service Priority Frequency/timing Number to send, Submission scheme Laboratory Examination Requested (test order, if applicable) Preparation and Shipping Instructions§
Aeromonas and related organisms Reference/surveillance At submitter’s convenience as needed any isolates you would like to have analyzed Identification, subtyping (CDC-10120) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Bacillus cereus Outbreak investigation ASAP^ as needed consult contact person for number to send Detection – foodborne outbreak (CDC-10104) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Campylobacter Outbreak investigation ASAP^ every suspected outbreak consult contact person for number to send Campylobacter subtyping (CDC-10127) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Campylobacter  (FoodNet Sites Only) NARMSC As scheduled at least quarterly Every 2nd, 3rd, 5th, or all isolates (varies by state) susceptibility testing for NARMS surveillance (10107) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Campylobacter, Helicobacter, and related organisms Reference/surveillance At submitter’s convenience as needed isolates that cannot be identified or atypical / rare isolates Identification (CDC-10126) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Clostridium botulinum and other botulinum producing species of Clostridia Reference/surveillance ASAP^ as needed consult contact person for number to send Botulinum toxin productin Clostridia subtyping (CDC-10133) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Clostridium perfringens Outbreak investigation ASAP^ As needed consult contact person for number to send Detection – foodborne outbreak (CDC-10111) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Cronobacter sakazakii Outbreak investigation ASAP^ Every suspected case Every isolate CDC-10123 Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Cronobacter species Reference/surveillance At submitter’s convenience, or ASAP^ if C. sakazakii has not been ruled out Every suspected case Any isolates requiring speciation CDC-10123 Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Enterobacteriaceae , other Reference/surveillance At submitter’s convenience as needed any isolates implicated in enteric disease Identification, serotyping, subtyping (CDC-10123) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Escherichia coli, diarrheagenic^^ Outbreak investigation ASAP^ Please send only when CDC requests 3 representative isolates Susceptibility testing (CDC-10116); other testing upon request Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Escherichia coli O157 NARMSC As scheduled at least quarterly every 20th** susceptibility testing for NARMS surveillance (10107) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Escherichia Reference/surveillance At submitter’s convenience as needed atypical isolates, rare serovars, or isolates that cannot be identified Identification, serotyping, subtyping, virulence profiling (CDC-10114) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Listeria monocytogenes  Outbreak investigation At submitter’s convenience * in every suspected outbreak 3 representative isolates from patients Identification, serotyping, subtyping (CDC-10129) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Listeria, species other than monocytogenes Reference/surveillance At submitter’s convenience * every time it is isolated/as soon as it is isolated any isolates obtained from a normally sterile body site Identification, serotyping, subtyping (CDC-10129) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Salmonella  Reference/surveillance  At submitter’s convenience as needed atypical isolates, rare serovars, or isolates that cannot be identified Salmonella identification and serotyping (CDC-10110) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Salmonella (all non-typhoidal serotypes and serotype Paratyphi B) NARMSC As scheduled at least quarterly every 20th** susceptibility testing for NARMS surveillance (10107) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Salmonella (all non-Typhi serotypes) Outbreak investigation ASAP^ Please send only when CDC requests 3 representative isolates Salmonella subtyping (CDC-10108) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Salmonella (typhoidal serotypes Paratyphi A, Paratyphi C and Typhi) NARMSC As scheduled at least quarterly ALL susceptibility testing for NARMS surveillance (10107) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Salmonella serotype Typhi Outbreak investigation ASAP^ every suspected outbreak consult contact person for number to send Salmonella subtyping (CDC-10108) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Shigella Reference/surveillance At submitter’s convenience as needed any isolates you would like to have analyzed Identification, subtyping (CDC-10114) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Shigella Outbreak investigation ASAP^ Please send only when CDC requests 3 representative isolates Susceptibility testing (CDC-10116) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Shigella NARMSC As scheduled at least quarterly every 20th** susceptibility testing for NARMS surveillance (10107) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Staphylococcus aureus Outbreak investigation  ASAP^ as needed consult contact person for number to send Detection – foodborne outbreak (CDC-10113) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
 Vibrio cholerae  Reference/surveillance  ASAP^ every time it is isolated/as soon as it is isolated All isolates Identification, virulence testing (10119) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Vibrio mimicus Reference/surveillance At submitter’s convenience at least quarterly All isolates Identification, virulence testing (CDC-10120) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Vibrio, non-cholerae Outbreak investigation ASAP^ every suspected outbreak All isolates Identification, subtyping (CDC-10122) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Vibrio, non-cholerae NARMSC As scheduled at least quarterly All isolates susceptibility testing for NARMS surveillance (10107) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Vibrio and related organisms Reference/surveillance At submitter’s convenience as needed any isolates requiring speciation and/or you would like to have analyzed Identification, subtyping (CDC-10120) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC
Yersinia (non-pestis ) Reference/surveillance At submitter’s convenience as needed any isolates requiring speciation and/or you would like to have analyzed Identification, serotyping, biotyping, subtyping (CDC-10123) Test Order | Submitting Specimens to CDC | Infectious Diseases Laboratories | CDC

§ Please submit specimens to CDC via CDC Specimen Test Order and Reporting (CSTOR) web portal or CDC 50.34 Specimen Submission Form: https://www.cdc.gov/laboratory/specimen-submission/form.html
^Isolates/specimens with a priority of “ASAP” should be shipped as soon as possible (within one business day of presumptive identification)
^^Escherichia coli, diarrheagenic = Shiga toxin-producing E. coli (STEC), Enterotoxigenic E. coli (ETEC), Enteropathogenic E. coli (EPEC), Enteroinvasive E. coli (EIEC) and Enteroaggregative E. coli (EAEC)
CNational Antimicrobial Resistance Monitoring System
* Laboratories that are NOT performing whole genome sequencing and submitting data in real time for Listeria should ship isolates ASAP
**For non-typhoidal Salmonella, Shigella, and E. coli O157 isolates included in NARMS surveillance, the 1st isolate collected each year should be submitted, followed by every 20th isolate

General inquiries: entericbacteria@cdc.gov, 404-639-3334

Public health officials (state, territorial, and CDC) can send general inquiries, and communicate about pending and current isolate requests by:

  • Email (entericbacteria@cdc.gov)
  • Phone call (Enteric Diseases Laboratory Branch at 404-639-3334)
  • Contacting specific personnel linked in Table 1 version 6.