Research Use Only 2019-Novel Coronavirus (2019-nCoV) Real-time RT-PCR Primer and Probe Information

NOT FOR DIAGNOSTIC USE

Reagents manufactured from these sequences may not be used for diagnostic testing under FDA’s authorization of the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel.
Only primers and probes labeled for EUA use and distributed by the International Reagent Resource may be used for diagnostic testing with the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel.

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These sequences are intended to be used for the purposes of respiratory virus surveillance and research. The recipient agrees to use them in compliance with all applicable laws and regulations. Every effort has  been made to assure the accuracy of the sequences, but CDC cannot provide any warranty regarding their accuracy.  The recipient can acknowledge the source of sequences in any oral presentations or written publications concerning the research project by referring to the Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

2019-Novel Coronavirus (2019-nCoV) Real-time rRT-PCR Panel Primers and Probes
Name Description Oligonucleotide Sequence (5’>3’) Label1 Working
Conc.
2019-nCoV_N1-F 2019-nCoV_N1 Forward Primer 5’-GAC CCC AAA ATC AGC GAA AT-3’ None 20 µM
2019-nCoV_N1-R 2019-nCoV_N1 Reverse Primer 5’-TCT GGT TAC TGC CAG TTG AAT CTG-3’ None 20 µM
2019-nCoV_N1-P 2019-nCoV_N1 Probe 5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3’ FAM, BHQ-1 5 µM
2019-nCoV_N2-F 2019-nCoV_N2 Forward Primer 5’-TTA CAA ACA TTG GCC GCA AA-3’ None 20 µM
2019-nCoV_N2-R 2019-nCoV_N2 Reverse Primer 5’-GCG CGA CAT TCC GAA GAA-3’ None 20 µM
2019-nCoV_N2-P 2019-nCoV_N2 Probe 5’-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3’ FAM, BHQ-1 5 µM
2019-nCoV_N3-F 2019-nCoV_N3 Forward Primer 5’-GGG AGC CTT GAA TAC ACC AAA A-3’ None 20 µM
2019-nCoV_N3-R 2019-nCoV_N3 Reverse Primer 5’-TGT AGC ACG ATT GCA GCA TTG-3’ None 20 µM
2019-nCoV_N3-P 2019-nCoV_N3 Probe 5’-FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1-3’ FAM, BHQ-1 5 µM
RP-F RNAse P Forward Primer 5’-AGA TTT GGA CCT GCG AGC G-3’ None 20 µM
RP-R RNAse P Reverse Primer 5’-GAG CGG CTG TCT CCA CAA GT-3’ None 20 µM
RP-P RNAse P
Probe
5’-FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1-3’ FAM, BHQ-1 5 µM

1TaqMan® probes are labeled at the 5′-end with the reporter molecule 6-carboxyfluorescein (FAM) and with the quencher, Black Hole Quencher 1 (BHQ-1) (Biosearch Technologies, Inc., Novato, CA) at the 3′-end.

Note: Oligonucleotide sequences are subject to future changes as the 2019-Novel Coronavirus evolves.