Research Use Only 2019-Novel Coronavirus (2019-nCoV) Real-time RT-PCR Primers and Probes

Updated June 6, 2020

NOT FOR VIRAL TESTING USE

Reagents manufactured from these sequences may not be used for viral testing under FDA’s authorization of the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel.
Only primers and probes labeled for EUA use and distributed by the International Reagent Resource may be used for viral testing with the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel.

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These sequences are intended to be used for the purposes of respiratory virus surveillance and research. The recipient agrees to use them in compliance with all applicable laws and regulations. Every effort has  been made to assure the accuracy of the sequences, but CDC cannot provide any warranty regarding their accuracy.  The recipient can acknowledge the source of sequences in any oral presentations or written publications concerning the research project by referring to the Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

2019-Novel Coronavirus (2019-nCoV) Real-time rRT-PCR Panel Primers and Probes
Name Description Oligonucleotide Sequence (5’>3’) Label1 Final
Conc.
2019-nCoV_N1-F 2019-nCoV_N1 Forward Primer GAC CCC AAA ATC AGC GAA AT None 500nM
2019-nCoV_N1-R 2019-nCoV_N1 Reverse Primer TCT GGT TAC TGC CAG TTG AAT CTG None 500nM
2019-nCoV_N1-P 2019-nCoV_N1 Probe FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1 FAM, BHQ-1 125nM
2019-nCoV_N1-P 2019-nCoV_N1 Probe FAM-ACC CCG CAT /ZEN/ TAC GTT TGG TGG ACC-3IABkFQ FAM, ZEN, 3IABkFQ 125nM
2019-nCoV_N2-F 2019-nCoV_N2 Forward Primer TTA CAA ACA TTG GCC GCA AA None 500nM
2019-nCoV_N2-R 2019-nCoV_N2 Reverse Primer GCG CGA CAT TCC GAA GAA None 500nM
2019-nCoV_N2-P 2019-nCoV_N2 Probe FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1 FAM, BHQ-1 125nM
2019-nCoV_N2-P 2019-nCoV_N2 Probe FAM-ACA ATT TGC /ZEN/ CCC CAG CGC TTC AG-3IABkF FAM, ZEN, 3IABkFQ 125nM
RP-F RNAse P Forward Primer AGA TTT GGA CCT GCG AGC G None 500nM
RP-R RNAse P Reverse Primer GAG CGG CTG TCT CCA CAA GT None 500nM
RP-P RNAse P
Probe
FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1 FAM, BHQ-1 125nM
RP-P RNAse P
Probe
FAM-TTC TGA CCT /ZEN/ GAA GGC TCT GCG CG-3IABkFQ FAM, ZEN, 3IABkFQ 125nM

1TaqMan® probes are labeled at the 5′-end with the reporter molecule 6-carboxyfluorescein (FAM) and with the quencher, Black Hole Quencher 1 (BHQ-1) (Biosearch Technologies, Inc., Novato, CA) at the 3′-end. TaqMan® probes can also be labeled at the 5′-end with the reporter molecule 6- carboxyfluorescein (FAM) and with a double quencher, ZEN™ Internal Quencher positioned between the ninth (9th) and tenth (10th) nucleotide base in the oligonucleotide sequence and Iowa Black® FQ (3IABkFQ) located at the 3’-end (Integrated DNA Technologies, Coralville, IA).

Note: Oligonucleotide sequences are subject to future changes as the 2019-Novel Coronavirus evolves.