Specimen Submission Guidelines for Pathologic Evaluation of Influenza Virus Infections
Viral antigens may be focal and sparsely distributed in patients with influenza and are most frequently detected in respiratory epithelium of large airways. Larger airways (particularly primary and segmental bronchi) have the highest yield for detection of influenza viruses by immunohistochemistry (IHC) staining. Collection of the appropriate tissues ensures the best chance of detecting the virus by immunohistochemical stains. Performance of specific immunohistochemical, molecular, or other assays will be determined using clinical and epidemiologic information provided by the submitter and the histopathologic features identified in the submitted tissue specimens.
Collection of Tissue Specimens
The preferred specimens would be a minimum of eight (8) blocks and fixed tissue specimens representing samples from the following pulmonary sites in addition to specimens from other organs showing pathology. Formalin-fixed paraffin-embedded blocks made from BAL can also be submitted for IHC staining. Fresh-frozen tissue may be submitted for culture and molecular-based assays. The recommended pulmonary sites include:
- Central (hilar) lung with segmental bronchi, right and left primary bronchi, trachea (proximal and distal)
- Representative pulmonary parenchyma from right and left lung.
- For patients with suspected myocarditis, encephalitis, or rhabdomyolysis, specimens should include myocardium (right and left ventricle), CNS (cerebral cortex, basal ganglia, pons, medulla, and cerebellum), and skeletal muscle, respectively.
- Specimens should be included from any other organ showing significant gross or microscopic pathology.
Submission of Specimens
Paraffin-embedded tissue blocks
In general, this is the preferred specimen and is especially important to submit in cases where tissues have been in formalin for a significant time. Prolonged fixation (>2 weeks) may interfere with some immunohistochemical and molecular diagnostic assays.
If available, we highly recommend that unprocessed tissues in 10% neutral buffered formalin be submitted in addition to paraffin blocks.
Although not optimal, if paraffin blocks are unavailable it may be possible to utilize unstained sections cut at 3–5 microns (10 slides per block) for immunohistochemistry and special stains but not molecular diagnostic assays (e.g. PCR).
Send separately on dry ice.
Electron Microscopy (EM) specimens
Samples fixed in glutaraldehyde and held in phosphate buffer. Sample containers are filled to the top with phosphate buffer and sent on wet ice. Do not freeze. Epoxy-embedded tissues are also accepted.
Please refer to our General Guidelines for shipping pathology specimens.
- Page last reviewed: December 7, 2012
- Page last updated: June 10, 2013
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