Submitting Specimens for Mycoplasma pneumoniae Testing

Key points

  • CDC primarily uses nucleic acid amplification tests to identify Mycoplasma pneumoniae.
  • CDC tests specimens identified as positive for M. pneumoniae for macrolide susceptibility.
  • CDC doesn't use culture or serological testing as routine diagnostic methods.
  • CDC only accepts specimens from state public health laboratories and other federal agencies.
Image of a box marked as containing an infectious substance.

Specimen acceptance criteria

CDC only accepts certain specimen types for M. pneumoniae testing from public health laboratories and other federal agencies. Healthcare providers should contact the public health laboratory for their jurisdiction to arrange for specimen submission.

Public health laboratories can forward approved specimens to CDC for specialized testing.

Specimen, documentation, packaging, and shipping

Specimen requirements vary by the specific molecular detection test requested.

The following links provide information on specimen, documentation, packaging, and shipping requirements:

Common rejection criteria

CDC's laboratory most commonly rejects specimens for M. pneumoniae testing for the following reasons:

  • Specimens stored, shipped, or received at incorrect temperatures.
  • Improperly labeled specimens.

Specimen collection protocol

Healthcare providers may collect different respiratory specimen types depending on the test that will be performed. Some studies suggest that oropharyngeal swab may be better for detection of M. pneumoniae compared to nasopharyngeal swab.12 Adherence to proper specimen collection technique is important for accurate test results.

For oropharyngeal swab specimens

Insert swab into the posterior pharynx and tonsillar areas. Rub swab over both tonsillar pillars and posterior oropharynx and avoid touching the tongue, teeth, and gums.

For nasopharyngeal swab specimens

Insert flexible shaft swab through the nares parallel to the palate (not upwards) until it contacts the nasopharynx. Indications of contact include encountering resistance and the distance equaling that from the ear to the patient's nostril. Gently rub and roll the swab.

Video: Collecting a nasopharyngeal swab specimen‎‎

During this video, the clinician collects samples by swabbing the posterior nasopharynx. While this video is not specific to M. pneumoniae, the swabbing technique is the same.

Resources

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