Resources for Laboratorians

2010 Guidelines

• 2010 Guidelines for the Prevention of Perinatal Group B Streptococcal Disease

Questions and Answers Relevant to Laboratorians

• Q&As About Implementing the 2010 Guidelines for Laboratorians

Algorithms

• Algorithm for recommended laboratory testing for prenatal screening for group B streptococcal (GBS) colonization [1 page].
• Customized Flowcharts for 2010 Guidelines (algorithms for pigmented broth, non-pigmented broth, NAAT, etc.)

Tables

• Procedures for collecting clinical specimens for culture of group B Streptococcus (GBS) at 35–37 weeks’ gestation [1 page].
• Procedures for processing clinical specimens for culture of group B Streptococcus (GBS) [1 page].
• Procedures for clindamycin and erythromycin susceptibility testing of group B streptococcal (GBS) isolates, when ordered for penicillin-allergic patients [1 page].
• Identification of group B Streptococcus (GBS) bacteriuria in pregnant women [1 page].

Slide Sets

• Early-onset Group B Streptococcal Disease Prevention: Procedures for Laboratories [5.34 MB, 54 pages] | PDF [2.26 MB, 54 pages] 508 compliant

Clinical Overview

• Clinical features, incidence, sequelae, trends…

Standard Operating Procedures for 2010 Guidelines for Laboratorians

Standard operating procedures, developed by the American Society for Microbiology (ASM) and CDC’s Streptococcus Laboratory, are available to assist laboratorians in processing group B Streptococcus specimens. The five documents are consistent with the recommendations in the 2010 GBS guidelines, but are tailored for use in different settings, depending on the procedures and assays used in each laboratory (e.g. pigmented or non-pigmented broth, sub-culture versus serologic or molecular methods, etc.).

Note: The Word documents can be modified to adapt for use in individual laboratories. The PDFs are provided simply for Section 508 accessibility.

• Using non-pigmented broth and only subculture for isolation and identification of GBS
  Word [9 pages] | PDF [8 pages]
• Using non-pigmented broth and serologic or molecular testing for identification of GBS
  Word [9 pages] | PDF [8 pages]
• Using pigmented broth and only subculture for isolation and identification of GBS
  Word [9 pages] | PDF [8 pages]
• Using pigmented broth and serologic or molecular testing for identification of GBS
  Word [9 pages] | PDF [8 pages]
• Using non-pigmented broth and only NAAT for identification of GBS
  Word [9 pages] | PDF [8 pages]

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