Module 2.7 – Wastewater-based variant tracking for SARS-CoV-2

Overview: This module describes the detection of SARS-CoV-2 RNA in municipal wastewater to provide early warning of increased community transmission and to inform clinical specimen selection for viral whole genome sequencing.

Posted: 11/17/21

Presenter: Brian Lowe, MPP (view bio)
Executive Director, Vermont Council on Rural Development

Download Presentation Slides pdf icon[PDF – 24 slides]

Further Reading

  1. National Wastewater Surveillance System (NWSS): A new public health tool to understand COVID-19 spread in a community.
    CDC, 2021.
  2. Public health interpretation and use of wastewater surveillance data.
    CDC, 2021.
  3. Wastewater tracking provides warning signs of local COVID-19 burdenexternal icon.
    APHL Blog.
  4. Droplet digital RT-PCR to detect SARS-CoV-2 variants of concern in wastewaterexternal icon.

Additional Resources

  1. Implementation of environmental surveillance for SARS-CoV-2 virus to support public health decisions: Opportunities and challengesexternal icon.
  2. Feasibility of neighborhood and building scale wastewater-based genomic epidemiology for pathogen surveillanceexternal icon.
    Spurbeck, et al. Sci Total Environ, 2021.
  3. COVID-19 containment on a college campus via wastewater-based epidemiology, targeted clinical testing and an interventionexternal icon.
    Betancourt WQ, et al. Sci Total Environ, 2021.
  4. Enumerating asymptomatic COVID-19 cases and estimating SARS-CoV-2 fecal shedding rates via wastewater-based epidemiologyexternal icon.
    Schmitz BW, et al. medRxiv, 2021.
  5. Wastewater-based epidemiology for tracking COVID-19 trend and variants of concern in Ohio, United Statesexternal icon.
    Ai, et al. medRxiv, 2021.
  6. The rapid assessment of aggregated wastewater samples for genomic surveillance of SARS-CoV-2 on a city-wide scaleexternal icon.
    Rouchka, et al. medRxiv, 2021.
  7. Measurement of SARS-CoV-2 RNA in wastewater tracks community infection dynamicsexternal icon.
    Nature Biotechnology, September 2020.
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Page last reviewed: November 17, 2021