Module 2.7 – Wastewater-based variant tracking for SARS-CoV-2

What to know

This module describes the detection of SARS-CoV-2 RNA in municipal wastewater to provide early warning of increased community transmission and to inform clinical specimen selection for viral whole genome sequencing.

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Overview

Presenter: Brian Lowe, MPP | Executive Director, Vermont Council on Rural Development
Download Presentation Slides [PDF - 24 slides]

Further Reading

  1. Wastewater tracking provides warning signs of local COVID-19 burden. APHL Blog.
  2. Droplet digital RT-PCR to detect SARS-CoV-2 variants of concern in wastewater.

Additional Resources

  1. Implementation of environmental surveillance for SARS-CoV-2 virus to support public health decisions: Opportunities and challenges.
  2. Feasibility of neighborhood and building scale wastewater-based genomic epidemiology for pathogen surveillance. Spurbeck, et al. Sci Total Environ, 2021.
  3. COVID-19 containment on a college campus via wastewater-based epidemiology, targeted clinical testing and an intervention.Betancourt WQ, et al. Sci Total Environ, 2021.
  4. Enumerating asymptomatic COVID-19 cases and estimating SARS-CoV-2 fecal shedding rates via wastewater-based epidemiology. Schmitz BW, et al. medRxiv, 2021.
  5. Wastewater-based epidemiology for tracking COVID-19 trend and variants of concern in Ohio, United States. Ai, et al. medRxiv, 2021.
  6. The rapid assessment of aggregated wastewater samples for genomic surveillance of SARS-CoV-2 on a city-wide scale. Rouchka, et al. medRxiv, 2021.
  7. Measurement of SARS-CoV-2 RNA in wastewater tracks community infection dynamics. Nature Biotechnology, September 2020.
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Content Source:
National Center for Emerging and Zoonotic Infectious Diseases (NCEZID)