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Laboratory Characterization

Download ABCs Isolate Characterization [2 pages]

Neisseria meningitidis

Identification and serogrouping by conventional microbiological methods. Confirmation of serogroup and species identification using real-time PCR

Multilocus sequence typing, pulsed field gel electrophoresis, and serosubtyping of isolates as part of special projects

Antimicrobial susceptibility testing of isolates

Haemophilus influenzae

Identification and serotyping (a-f) by conventional microbiological methods

Confirmation of serotype and species using real-time PCR

group A Streptococcus

T and emm typing of all isolates

Sof sequence typing of a subset of isolates

Antimicrobial susceptibility testing of a subset of isolates
group B Streptococcus

Serotyping of isolates from select surveillance areas

Antimicrobial susceptibility testing of isolates from select surveillance areas

Streptococcus pneumoniae

Antimicrobial susceptibility testing of all isolates

Serotyping of all isolates

Progressing toward clonal typing of all isolates using MLST

Characterization of resistance mechanisms of selected strains based on phenotypic resistance patterns

Methicillin resistant Staphylococcus aureus (MRSA)

Confirmation of isolate purity

Confirmatory identification of Staphylococcus aureus by detection of nuc gene by real-time PCR

Antimicrobial susceptibility testing performed using a standard panel

Toxin testing consisting at a minimum of determining presence of PVL and TSST-1

SCCmec cassette typing performed using real-time PCR

Spa typing used to determine strain type of all MRSA isolates

Isolates can be requested for research purposes through the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) program.

Bordetella pertussis

Antimicrobial resistance screening (erythromycin and azithromycin)

Pulsed-field gel electrophoresis (PFGE)

Molecular characterization (MLVA and MLST)

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