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NIOSH Home > Safety and Health Topics >Skin Exposures and Effects >Occupational & Environmental Exposures of Skin to Chemicals- 2005> Abstracts

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Abstract for Short Course 1.2

 

 

In vivo studies for prediction of percutaneous penetration

Eva Benfeldt
Department of Dermatology, University of Copenhagen, Bispebjerg Hospital, Denmark. benfeldt@post5.tele.dk

For in vivo studies of drug concentrations in the skin/percutaneous penetration a variety of techniques exist. Following a review of these options, including the limitations and advantages of each method, this short course will focus on methods used and results obtained in human experiments. Two methods will be discussed in more depth:

Tape stripping technique is used in studies of the distribution of topical agents (drugs, sunscreens, toxic substances) within the stratum corneum (SC). Recent method development has demonstrated the depth profile of substance penetration within the SC. A linear relationship between the drug content in the SC 30 min after topical application and the total amount absorbed systemically has been found in some studies, but is absent in other studies.

Microdialysis sampling can be performed in the dermis and subcutaneous ( s.c. ) tissue, and probe depth can be measured by ultrasound scanning. The technique is used for pharmacokinetic and/or pharmacodynamic studies in the skin and s.c. tissue after topical, transdermal, regional, systemic and intraprobe drug delivery. Simultaneous sampling in a remote location can measure the systemic distribution of topically applied drugs.

The use of microdialysis sampling of cutaneous drug penetration following topical drug exposure is presented, along with the effect of several types of barrier perturbation on drug penetration.

The results of recent studies, where intradermal microdialysis has been employed simultaneously with the tape-stripping methodology in human volunteers, will be presented. In particular, the source(s) of variability in microdialysis sampling, identified by statistical analyses, will be discussed. The advantages and limitations of the 2 methods used will be considered.

Content last modified: 14 May 2005

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