NORA Manufacturing Sector Strategic Goals
927ZJFB - Preparation of Length-Classified Fibers for Toxicology StudiesStart Date: 4/1/2009
End Date: 9/30/2013
Principal Investigator (PI)Name: Leonid Turkevich
Funded By: NIOSH
Primary Goals Addressed5.0 , 6.0
Secondary Goal AddressedNone
Attributed to Manufacturing50%
The objective of this project is to develop and construct an instrument facility capable of producing length-selected fibers (e.g. asbestos) in quantities sufficient for in vivo and in vitro toxicological studies. Successful instrument development will enable toxicology studies to test directly the importance of fiber length in asbestos-related diseases: asbestosis, lung cancer, mesothelioma.
NIOSH has developed a dielectrophoresis technique for generating size-selected fractions of fibers by length [Baron et al. 1994]. The technique, in its current analytical implementation, is only capable of processing small quantities of fibers.
The objective of this project is to develop a technique capable of producing length-selected fibers (e.g. asbestos) in quantities sufficient for in vivo and in vitro toxicological studies. This work will enable toxicology studies to directly test the importance of fiber length in asbestos-related diseases: asbestosis, lung cancer, mesothelioma.
Fiber length has long been suspected (Stanton hypothesis) as being a crucial parameter which determines various toxicological responses (fibrosis, lung cancer, mesothelioma) to the presence of asbestos in the lung. Direct toxicological testing has been hampered by the inability to prepare significant quantities of length-classified asbestos samples.