NORA Manufacturing Sector Strategic Goals
927Z6RV - Dermal penetration of metal working fluid componentsStart Date: 10/1/2006
End Date: 9/30/2009
Principal Investigator (PI)Name: H.frederick Frasch
Funded By: NIOSH
Primary Goal Addressed9.0
Secondary Goal AddressedNone
Attributed to Manufacturing100%
The goal of this project is to obtain data on the permeation through skin of selected MWF's, using hairless guinea pig skin as a surrogate for human. Metal working fluids (MWF's) are used in the machining industry to reduce friction between the metal surface and the cutting blade. The machining process generates a fine mist that can be inhaled and deposited on the worker's skin. Occupational exposures to MWF may cause a variety of adverse dermal health effects. Metal working fluid components will be selected for study based on known or suspected potential for adverse dermal effects, including irritant and allergic dermatitis and toxicity to the body. Dermal absorption rates will be compared between new, unused fluid and used fluid obtained from an industrial machining operation.
Dermal exposures to metal working fluids occur in industrial settings. The goal of this research is to quantify the dermal penetration of potentially toxic components of metal working fluids. In-vitro skin penetration experiments will comprise the core experimental component of the project. Human surgical waste skin (panniculectomies or breast reductions) will be used. The chemical of interest will be placed on the skin membranes in our diffusion cell apparatus. The amount of chemical penetrating the skin will be monitored until a steady-state rate of absorption can be determined. LCMS or GC methods will be developed to quantify MWF components alone, in aqueous solutions, or within the MWF matrix.
1. New insight into dermal absorption and the potential for dermatitis of MWF's. Knowledge of the specific MWF components that are implicated in dermatitis, and the potential for dermal absorption of systemic toxins, will enable users of MWF to make more informed decisions regarding their use. Furthermore, it may encourage manufacturers of MWF to alter their formulation by replacing dermal toxins with less harmful components.
Metal working fluids (MWF's) are used in the machining industry to reduce friction between the metal surface and the cutting blade. The machining process generates a fine aerosol that can be inhaled and deposited on the worker's skin. NIOSH estimates that some 1.2 million workers are potentially exposed. Occupational exposures to MWF may cause a variety of adverse respiratory and dermal health effects, with dermal exposures are most commonly associated with allergic and irritant dermatitis. In addition, MWF's contain systemic toxins for which the skin could serve as an entry portal. It is therefore important to understand the potential for dermal penetration of metal working fluid components. MWF's are proprietary formulations and most components unfortunately are not listed on Material Safety Data Sheets or other sources. Therefore the precise composition of a MWF is unknown. Recent methodological advances within NIOSH (DART) now permit the quantitative identification of MWF components. It is therefore now possible to study the skin penetration of these specific compounds, alone or within the MWF matrix. The goal of this project is to gain knowledge of the dermal penetration capacity of metal working fluids and their components. This knowledge will enhance understanding of the potential for these complex mixtures to cause allergic and contact dermatitis, and will aid in the dermal risk assessment of these fluids.