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HIV Screening & Testing

Male Scientist testingThe listed documents provide guidance about HIV screening and diagnostic
testing of patients and clients and HIV screening of donated tissue and organs.
This guidance is intended for clinicians and other HIV testing providers. HIV screening and testing guidance continues to evolve with changes in testing technology and methods to reach persons who can benefit from these services. Currently, CDC is updating recommendations for HIV testing outside of healthcare facilities and diagnostic HIV testing in laboratories in collaboration with federal and non-federal partner organizations. These recommendations will be posted on this page in the future.


  • HIV Testing

  • Donors of Blood, Organs and Other Tissues

  • Laboratory Testing Recommendations

    The document “Laboratory Testing for the Diagnosis of HIV Infection: Updated Recommendations” updates recommendations for HIV testing by laboratories in the United States and offers approaches for reporting test results to persons ordering HIV tests and to public health authorities. In brief, testing begins with a combination immunoassay that detects HIV-1 and HIV-2 antibodies and HIV-1 p24 antigen. All specimens reactive on this initial assay undergo supplemental testing with an immunoassay that differentiates HIV-1 from HIV-2 antibodies. Specimens that are reactive on the initial immunoassay and nonreactive or indeterminate on the antibody differentiation assay proceed to HIV-1 nucleic acid testing for resolution.





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