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Guideline for the Prevention and Control of Norovirus Gastroenteritis Outbreaks in Healthcare Settings, 2011

Download the complete PDF version Guideline for the Prevention and Control of Norovirus Gastroenteritis Outbreaks in Healthcare Settings, 2011 [PDF - 676 KB] and Appendices [PDF - 3.48 MB].

Q2: What are the best methods to identify a norovirus outbreak in a healthcare setting?

Diagnostic methods – Fecal specimens

NASBA

Author, Yr (Ref)	Study Design Quality	Study Objective	Population and Setting N	Results	Comments	Ref ID_Data extracted by
Jean, J; 2003 143	Diagnostic study 2	To evaluate the sensitivity of NASBA primers specific for the GII norovirus adapted for RT-PCR and the effect of transcriptional enhancement (TE) both followed by electrochemiluminescence (ECL).	Stool specimens from regional gastroenteritis outbreaks. Study conducted in North Carolina Not reported	Sensitivity of NASBA derived RT-PCR Comparable to other RT-PCR protocols. Consistent detection of viral RNA by RT-PCR was obtained up to approximately -7 log10 dilution with ECL readings ranging from 3.2 to 3.6 log10 Sensitivity of NASBA derived RT-PCR/TE A detection limit of ≥1 log10 was observed with ECL readings ranging from 4.3 to >7.0 log10	Power and sample size not reported	5780_RA
Greene, S; 2003 144	Diagnostic study 1,2	To determine the test characteristics of a rapid NASBA when compared with RT-PCR for the detection of Norwalk-like viruses (NLV)	Volunteers challenged with norovirus. Demographics not reported. Study setting unclear. 15 stool specimens	Detection limits The NASBA assay could consistently detect 105-102 detectable units of NLV RNA in a stool filtrate. Cross-reactivity Cross-reactivity studies with a representative panel of other enteric pathogens were negative Sensitivity 100% Specificity 50% Accuracy 67%	Power and sample size not reported	856_RA

Author, Yr (Ref)

Study Design Quality

Study Objective

Population and Setting N

Results

Comments

Ref ID_Data extracted by

Jean, J; 2003 143

Diagnostic study

To evaluate the sensitivity of NASBA primers specific for the GII norovirus adapted for RT-PCR and the effect of transcriptional enhancement (TE) both followed by electrochemiluminescence (ECL).

Stool specimens from regional

gastroenteritis outbreaks. Study conducted in North Carolina

Not reported

Sensitivity of NASBA derived RT-PCR
Comparable to other RT-PCR protocols. Consistent detection of viral RNA by RT-PCR was obtained up to approximately -7 log10 dilution with ECL readings ranging from 3.2 to 3.6 log10

Sensitivity of NASBA derived RT-PCR/TE
A detection limit of ≥1 log10 was observed with ECL readings ranging from 4.3 to >7.0 log10

Power and sample size not reported

5780_RA

Greene, S; 2003 144

Diagnostic study

1,2

To determine the test characteristics of a rapid NASBA when compared with RT-PCR for the detection of Norwalk-like viruses (NLV)

Volunteers challenged with norovirus. Demographics not reported. Study setting unclear.

15 stool specimens

Detection limits
The NASBA assay could consistently detect 105-102 detectable units of NLV RNA in a stool filtrate.

Cross-reactivity
Cross-reactivity studies with a representative panel of other enteric pathogens were negative

Sensitivity
100%

Specificity
50%

Accuracy
67%

Power and sample size not reported

856_RA

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Healthcare Infection Control Practices Advisory Committee (HICPAC)

Guideline for the Prevention and Control of Norovirus Gastroenteritis Outbreaks in Healthcare Settings, 2011

Q2: What are the best methods to identify a norovirus outbreak in a healthcare setting?

Diagnostic methods – Fecal specimens

NASBA

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