o-Anisidine

RTECS #

BZ5410000

CAS #

90-04-0
See: NMAM or OSHA Methods

Updated

December 2018

Molecular Weight

123.17

Molecular Formula

C₇H₉NO

Synonyms

1-Amino-2-methoxybenzene
2-Aminoanisole
2-Anisidine
2-Methoxy-1-aminobenzene
2-Methoxyaniline
2-Methoxybenzenamine
2-Methoxyphenylamine
Benzenamine, 2-methoxy- (9CI)
o-Aminoanisole
o-Anisidine (ACGIH:OSHA)
o-Anisylamine
o-Methoxyaniline
o-Methoxyphenylamine

Mutation Data and Reference

System Test	Route/Organism/Tissue	Dose	Reference
Cytogenetic Analysis	ovary/hamster	1200 mg/L	EMMUEG 10(Suppl 10),1,1987
DNA Damage	oral/rat	1800 mg/kg/3D- intermittent	MUREAV 786-788,172,2015
DNA Damage	lymphocyte/mouse	1250 µmol/L	MUREAV 203,155,1988
DNA Damage	oral/mouse	690 mg/kg	MUREAV 412,155,1998
DNA inhibition	oral/mouse	200 mg/kg	MUREAV 46,305,1977
DNA repair	/Escherichia coli	31600 µmol/L	MUREAV 272,145,1992
gene conversion and mitotic recombination	/Drosophila melanogaster	1 mmol/L	MUREAV 514,193,2002
host-mediated assay	Escherichia coli/mouse	310 mg/kg	MUREAV 272,161,1992
mutation in mammalian somatic cells	lymphocyte/mouse	2 mmol/L	MUTAEX 3,193,1988
mutation in microorganisms	/Salmonella typhimurium	333 µg/plate (-enzymatic activation step)	IMEMDT 27,63,1982
mutation in microorganisms	lymphocyte/mouse	1 mmol/L (+enzymatic activation step)	MUTAEX 3,193,1988
sister chromatid exchange	ovary/hamster	38 mg/L	EMMUEG 10(Suppl 10),1,1987

Acute Toxicity Data and References

Route/Organism	Dose	Effect	Reference
In Vitro/Human, leukemia cells	Inhibitor Concentration (50 percent kill): 13.2 mmol/L/24H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.	TIVIEQ 40,153,2017
In Vitro/Human, leukemia cells	Inhibitor Concentration Low: 12.5 mmol/L/24H	In Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)	TIVIEQ 40,153,2017
In Vitro/Human, leukemia cells	Inhibitor Concentration Low: 0.5 mmol/L/24H	Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation	TIVIEQ 40,153,2017
In Vitro/Human, liver tumor	Inhibitor Concentration Low: 6.3 mmol/L/24H	In Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)	TIVIEQ 40,153,2017
In Vitro/Human, liver tumor	Inhibitor Concentration Low: 5 mmol/L/3H	In Vitro Toxicity Studies: Other assays	TIVIEQ 40,153,2017
In Vitro/Human, liver tumor	Inhibitor Concentration Low: 1.6 mmol/L/3H	Biochemical: Metabolism (intermediary): Effect on mitochondrial function	TIVIEQ 40,153,2017
In Vitro/Human, liver tumor	Inhibitor Concentration Low: 1.5 mmol/L/24H	In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc.	TIVIEQ 40,153,2017
In Vitro/Human, liver tumor	Inhibitor Concentration Low: 0.5 mmol/L/24H	Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation	TIVIEQ 40,153,2017
In Vitro/Human, liver tumor	Inhibitor Concentration (50 percent kill): 6.9 mmol/L/24H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.	TIVIEQ 40,153,2017
In Vitro/Mouse, liver	Inhibitor Concentration Low: 6.3 mmol/L/24H	In Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)	TIVIEQ 40,153,2017
In Vitro/Mouse, liver	Inhibitor Concentration (50 percent kill): 13 mmol/L/24H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.	TIVIEQ 40,153,2017
In Vitro/Mouse, liver	Inhibitor Concentration Low: 2.5 mmol/L/3H	In Vitro Toxicity Studies: Other assays	TIVIEQ 40,153,2017
oral/mouse	lethal dose (50 percent kill): 1400 mg/kg	Kidney, Ureter, and Bladder: Other changes Blood: Normocytic anemia Blood: Other changes	IMEMDT 27,63,1982
oral/rabbit	lethal dose (50 percent kill): 870 mg/kg	Kidney, Ureter, and Bladder: Other changes Blood: Normocytic anemia Blood: Other changes	IMEMDT 27,63,1982
oral/rat	lethal dose (50 percent kill): 1150 mg/kg		TLSMA6 128,14,1979
oral/wild bird	lethal dose (50 percent kill): 422 mg/kg		AECTCV 12,355,1983

Reviews

Organization	Standard	Reference
American Conference of Governmental Industrial Hygienists (ACGIH)	Threshold Limit Value-time-weighted average 0.5 mg/m³ (skin)	DTLVS* TLV/BEI,2013
American Conference of Governmental Industrial Hygienists (ACGIH)	Threshold Limit Value-Confirmed animal carcinogen	DTLVS* TLV/BEI,2013
International Agency for Research on Cancer (IARC)	Cancer Review:Animal Sufficient Evidence	IMEMDT 73,49,1999
International Agency for Research on Cancer (IARC)	Cancer Review:Human No Adequate Data	IMEMDT 27,63,1982
International Agency for Research on Cancer (IARC)	Cancer Review:Human Inadequate Evidence	IMEMDT 73,49,1999
International Agency for Research on Cancer (IARC)	Cancer Review:Group 2B	IMEMDT 73,49,1999
TOXICOLOGY REVIEW		ENTOX* -,104,2005
TOXICOLOGY REVIEW		MUREAV 752,99,2013
TOXICOLOGY REVIEW		MUREAV 786-788,14,2015
TOXICOLOGY REVIEW		MUREAV 786-788,182,2015
TOXICOLOGY REVIEW		RTOPDW 55,52,2009

Standards and Regulations

Organization	Standard	Reference
Mine Safety and Health Administration (MSHA) STANDARD-air	time-weighted average 0.1 ppm (0.5 mg/m³) (skin)	DTLWS* 3,10,1973
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV;
Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV
Occupational Exposure Limit-AUSTRALIA	time-weighted average 0.1 ppm (0.5 mg/m³), JAN1993
Occupational Exposure Limit-AUSTRIA	TRK-TMW 0.1 ppm (0.5 mg/m³);KZW 0.2 ppm (1 mg/m³), skin, 2007
Occupational Exposure Limit-BELGIUM	time-weighted average 0.1 ppm (0.5 mg/m³), Skin, MAR2002
Occupational Exposure Limit-DENMARK	time-weighted average 0.1 ppm (0.5 mg/m³), skin, carc, MAY2011
Occupational Exposure Limit-FINLAND	time-weighted average 0.1 ppm (0.5 mg/m³), short term exposure limit 0.3 ppm (1.5 mg/m³), skin, NOV2011
Occupational Exposure Limit-FRANCE	VME 0.1 ppm (0.5 mg/m³), Skin, C2 Carcinogen, FEB2006
Occupational Exposure Limit-HUNGARY	ceiling concentration 0.5 mg/m³, Carcinogen, SEP2000
Occupational Exposure Limit-ICELAND	time-weighted average 0.1 ppm (0.5 mg/m³), carc, skin, NOV2011
Occupational Exposure Limit-INDIA	time-weighted average 0.1 ppm (0.5 mg/m³), Skin, JAN1993
Occupational Exposure Limit-JAPAN	Occupational Exposure Limit 0.1 ppm (0.5 mg/m³), skin, 2B carc, MAY2012
Occupational Exposure Limit-KOREA	time-weighted average 0.5 mg/m³, skin, 2006
Occupational Exposure Limit-MEXICO	time-weighted average 0.1 ppm (0.5 mg/m³) (skin), 2004
Occupational Exposure Limit-NEW ZEALAND	time-weighted average 0.1 ppm (0.5 mg/m³), skin, JAN2002
Occupational Exposure Limit-NORWAY	time-weighted average 0.1 ppm (0.5 mg/m³), JAN1999
Occupational Exposure Limit-PERU	time-weighted average 0.1 ppm (0.5 mg/m³), JUL2005
Occupational Exposure Limit-POLAND	MAC(time-weighted average) 0.5 mg/m³, MAC(short term exposure limit) 1 mg/m³, JAN1999
Occupational Exposure Limit-RUSSIA	short term exposure limit 1 mg/m³, Skin, JUN2003
Occupational Exposure Limit-SWITZERLAND	MAK-week 0.1 ppm (0.5 mg/m³), carc 2, skin, JAN2011
Occupational Exposure Limit-THE PHILIPPINES	time-weighted average 0.5 mg/m³, Skin, JAN1993
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction)	8H time-weighted average 0.5 mg/m³ (skin)	CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors)	8H time-weighted average 0.5 mg/m³ (skin)	CFRGBR 41,50-204.50,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry)	8H time-weighted average 0.5 mg/m³ (skin)	CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards)	8H time-weighted average 0.5 mg/m³ (skin)	CFRGBR 29,1915.1000,1993

NIOSH Documentation and Surveillance

Organization	Standard	Reference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO o-ANISIDINE-air	10H Carcinogen time-weighted average 0.5 mg/m³ (Sk)	NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983	Hazard Code X8302; Number of Industries 1; Total Number of Facilities 50; Number of Occupations 3; Total Number of Employees Exposed 705

Status in Federal Agencies

Organization	Reference
EPA GENETOX PROGRAM 1988, Inconclusive: Histidine reversion-Ames test
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NIOSH Analytical Method, 1994: Anisidine, 2514
NTP 14th Report on Carcinogens,2016:Reasonably anticipated to be human carcinogen
On EPA IRIS database

Page last reviewed: November 16, 2018

Content source: National Institute for Occupational Safety and Health