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Registry of Toxic Effects of Chemical Substances (RTECS)

Methane, dichloro-

RTECS #

PA8050000

CAS #

75-09-2

Updated

October 2017

Molecular Weight

84.93

Molecular Formula

CH2Cl2

Synonyms

Aerothene MM
Chlorure de methylene (French)
Dichloromethane
Dichloromethane (DOT:OSHA)
F 30 (chlorocarbon)
Freon 30
HCC 30
Khladon 30
Methane dichloride
Methylene bichloride
Methylene chloride
Methylene chloride (ACGIH:OSHA)
Methylene dichloride
Metylenu chlorek (Polish)
Narkotil
NCI-C50102
R 30
R30 (refrigerant)
RCRA waste number U080
Solaesthin
Soleana VDA
Solmethine

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
eye /rabbit 162 mg moderateEJTXAZ 9,171,1976
eye /rabbit 10 mg mildTXCYAC 6,173,1976
eye /rabbit 500 mg/24H mild85JCAE -,88,1986
skin /rabbit 810 mg/24H severeEJTXAZ 9,171,1976
skin /rabbit 100 mg/24H moderate85JCAE -,88,1986

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysisinhalation/mouse27760 mg/m3/6H/2W- intermittentEMMUEG 15,221,1990
Cytogenetic Analysislung/hamster1 µmol/LTECSDY 13,205,1987
Cytogenetic Analysisovary/hamster6628 mg/LMUREAV 116,361,1983
DNA Damageovary/hamster3000 ppmMUREAV 367,143,1996
DNA Damageliver/mouse400 µmol/LCRNGDP 15,991,1994
DNA Damageinhalation/mouse4000 ppm/6H-continuousCRNGDP 15,991,1994
DNA Damageoral/mouse1720 mg/kgMUREAV 419,13,1998
DNA Damageoral/rat1275 mg/kgTCMUD8 9,61,1989
DNA Damageliver/rat30 µmol/LCRNGDP 15,991,1994
DNA Damagelung/human1000 µmol/L/3HMUREAV 538,41,2003
DNA Damagefibroblast/hamster5 mmol/L/2HMUREAV 577S,1,2005
DNA inhibitionfibroblast/human5000 ppm/1H-continuousMUREAV 81,203,1981
DNA inhibitionlung/hamster5000 ppm/1H-continuousMUREAV 81,203,1981
micronucleus testinhalation/mouse27760 mg/m3/6H/2W- intermittentEMMUEG 15,221,1990
morphological transformembryo/rat160 µmol/LITCSAF 14,290,1978
morphological transformembryo/hamster1300 µL/plateEVSRBT 25,75,1982
morphological transformfibroblast/mouse0.1 mg/L/21D (-enzymatic activation step)EMMUEG 35,300,2000
mutation in mammalian somatic cellsovary/hamster3000 ppmMUREAV 367,143,1996
mutation in microorganisms/Salmonella typhimurium5700 ppm (+/-enzymatic activation step)MUREAV 56,245,1978
mutation in microorganisms/Escherichia coli6300 ppm (+/-enzymatic activation step)EMMUEG 20,211,1992
other mutation test systemsovary/hamster6628 mg/LMUREAV 116,361,1983
phage inhibition capacity/Escherichia coli8250 µg/wellMUREAV 260,349,1991
sex chromosome loss and nondisjunctionoral/Drosophila melanogaster125 mmol/LMUREAV 90,91,1981
sister chromatid exchangelung/hamster5000 ppm/1H-continuousMUREAV 81,203,1981
sister chromatid exchangeinhalation/mouse13880 mg/m3/6H/2W- intermittentEMMUEG 15,221,1990
sister chromatid exchange/human500 ppm/72HEMMUEG 48,625,2007
sister chromatid exchangeother cell types/human30 ppm/72HTOLED5 199,218,2010

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
inhalation/rat 4500 ppm/24H (1-17D pregnant)Reproductive: Effects on newborn: BehavioralTXAPA9 52,29,1980
inhalation/rat 1250 ppm/7H (6-15D pregnant)Reproductive: Specific developmental abnormalities: Musculoskeletal system

Reproductive: Specific developmental abnormalities: Urogenital system
TXAPA9 32,84,1975
inhalation/mouse 1250 ppm/7H (6-15D pregnant)Reproductive: Specific developmental abnormalities: Musculoskeletal systemTXAPA9 32,84,1975

Tumorigenic Data and References

Route/OrganismDoseEffectReference
inhalation/mouse lowest published toxic concentration: 2000 ppm/5H/2Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Lung, Thorax, or Respiration: Tumors
NTPTR* NTP-TR-306,1986
inhalation/mouse lowest published toxic concentration: 122400 mg/kg/102W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Lung, Thorax, or Respiration: Tumors

Liver: Tumors
NTIS** PB86-187903/AS
inhalation/mouse lowest published toxic concentration: 30239.3 mg/kg/13W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Lung, Thorax, or Respiration: Tumors
MUREAV 746,135,2012
inhalation/mouse lowest published toxic concentration: 92337.7 mg/kg/13W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Lung, Thorax, or Respiration: Tumors
MUREAV 746,135,2012
inhalation/rat lowest published toxic concentration: 122400 mg/kg/103W- intermittentTumorigenic: Neoplastic by RTECS criteria

Skin and Appendages: Tumors
NTIS** PB86-187903/AS
inhalation/rat lowest published toxic concentration: 102000 mg/kg/102W- intermittentTumorigenic: Neoplastic by RTECS criteria

Skin and Appendages: Tumors

Blood: Leukemia
NTIS** PB86-187903/AS
inhalation/rat lowest published toxic concentration: 3500 ppm/6H/2Y- intermittentTumorigenic: Carcinogenic by RTECS criteria

Endocrine: Tumors
FAATDF 4,30,1984

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
eye/rabbit lowest published toxic dose: 0.25 mL/kgEye: Increased intraocular pressure

Eye: Conjunctiva irritation

Eye: Corneal damage
VCVGH* -,323,1990
In Vitro/Chicken, neurons Inhibitor Concentration (50 percent kill): 125548 µmol/L/21HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 7,653,1993
In Vitro/Chicken, neurons Inhibitor Concentration (10 percent kill): 32249 µmol/L/21HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 7,653,1993
In Vitro/Chicken, neurons Inhibitor Concentration (50 percent kill): 87758 µmol/L/20HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 7,653,1993
In Vitro/Chicken, neurons Inhibitor Concentration (10 percent kill): 25310 µmol/L/20HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 7,653,1993
In Vitro/Human, leukemia cells Inhibitor Concentration (20 percent kill): 16.54 mmol/L/3HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.

In Vitro Toxicity Studies: Other assays
TXCYAC 271,122,2010
In Vitro/Human, leukemia cells Inhibitor Concentration (50 percent kill): 53.86 mmol/L/3HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.

In Vitro Toxicity Studies: Other assays
TXCYAC 271,122,2010
inhalation/cat Lowest published lethal concentration: 43400 mg/m3/4.5HBehavioral: General anesthetic

Behavioral: Ataxia

Nutritional and Gross Metabolic: Body temperature decrease
AHBAAM 116,131,1936
inhalation/Dog Lowest published lethal concentration: 14108 ppm/7HEye: Miosis (pupilliary constriction)

Vascular: BP lowering not characterized in autonomic section

Lung, Thorax, or Respiration: Dyspnea
NIHBAZ 191,1,1949
inhalation/guinea pig Lowest published lethal concentration: 5000 ppm/2HFLCRAP 1,197,1967
inhalation/guinea pig lethal concentration (50 percent kill): 40200 mg/m3/6HVCVGH* -,320,1990
inhalation/guinea pig lowest published toxic concentration: 21000 mg/m3/45MBehavioral: General anestheticVCVGH* -,320,1990
inhalation/human lowest published toxic concentration: 69600 mg/m3/30MBehavioral: General anestheticVCVGH* -,321,1990
inhalation/human lowest published toxic concentration: 25000 mg/m3/8MPeripheral Nerve and Sensation: Paresthesia

Eye: Conjunctiva irritation
VCVGH* -,321,1990
inhalation/human lowest published toxic concentration: 8000 mg/m3/30MGastrointestinal: Nausea or vomitingVCVGH* -,321,1990
inhalation/human lowest published toxic concentration: 500 ppm/1Y- intermittentBehavioral: Altered sleep time (including change in righting reflex)

Behavioral: Somnolence (general depressed activity)

Cardiac: Change in rate
ABHYAE 43,1123,1968
inhalation/human lowest published toxic concentration: 500 ppm/8HBehavioral: EuphoriaSCIEAS 176,295,1972
inhalation/mouse lethal concentration (50 percent kill): 14400 ppm/7HNIHBAZ 191,1,1949
inhalation/mouse lowest published toxic concentration: 32500 mg/m3/2HBehavioral: General anestheticVCVGH* -,307,1990
inhalation/mouse lethal concentration (50 percent kill): 49100 mg/m3/6HVCVGH* -,320,1990
inhalation/mouse lethal concentration (16 percent kill): 53000 mg/m3/6HVCVGH* -,320,1990
inhalation/mouse lethal concentration (50 percent kill): 54000 mg/m3/2HVCVGH* -,320,1990
inhalation/mouse lethal concentration (84 percent kill): 75000 mg/m3/2HVCVGH* -,320,1990
inhalation/mouse lethal concentration (50 percent kill): 56220 mg/m3/7HVCVGH* -,320,1990
inhalation/mouse lowest published toxic concentration: 35 gm/m3/2HBrain and Coverings: Changes in circulation

Brain and Coverings: Other degenerative changes

Behavioral: General anesthetic
TPKVAL 1,96,1961
inhalation/mouse lowest published toxic concentration: 15 gm/m3/2HBehavioral: General anesthetic

Behavioral: Convulsions or effect on seizure threshold

Behavioral: Ataxia
TPKVAL 1,96,1961
inhalation/mouse lethal concentration (50 percent kill): 25 gm/m3/2HBehavioral: Alteration of classical conditioning

Liver: Fatty liver degeneration

Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)
TPKVAL 1,96,1961
inhalation/mouse lowest published toxic concentration: 10 gm/m3/2HTPKVAL 1,96,1961
inhalation/mouse lowest published toxic concentration: 40000 mg/m3Behavioral: General anestheticVCVGH* -,320,1990
inhalation/mouse lowest published toxic concentration: 17000 mg/m3/2HBehavioral: Somnolence (general depressed activity)VCVGH* -,320,1990
inhalation/mouse Lowest published lethal concentration: 30 gm/m3/2HSkin: After systemic exposure: Dermatitis, otherTPKVAL 1,96,1961
inhalation/mouse Lowest published lethal concentration: 50 gm/m3/2HBehavioral: Ataxia

Lung, Thorax, or Respiration: Respiratory obstruction

Liver: Fatty liver degeneration
TPKVAL 1,96,1961
inhalation/rabbit Lowest published lethal concentration: 10000 ppm/7HJIHTAB 26,8,1944
inhalation/rat lethal concentration (50 percent kill): 76000 mg/m3/4HVCVGH* -,320,1990
inhalation/rat lethal concentration (50 percent kill): 52000 mg/m3/6HVCVGH* -,320,1990
inhalation/rat Lowest published lethal concentration: 52000 mg/m3Blood: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other enzymes
TPKVAL 15,64,1979
inhalation/rat lowest published toxic concentration: 1000 mg/m3/30MEndocrine: Adrenal cortex hyperplasia

Behavioral: Alteration of classical conditioning
VCVGH* -,320,1990
inhalation/rat lowest published toxic concentration: 50000 mg/m3Behavioral: General anestheticVCVGH* -,320,1990
inhalation/rat lowest published toxic concentration: 1 gm/m3/1HTPKVAL 1,96,1961
intraperitoneal/Dog lethal dose (50 percent kill): 1274 mg/kgTXAPA9 10,119,1967
intraperitoneal/mouse lethal dose (50 percent kill): 437 mg/kgAGGHAR 18,109,1960
intraperitoneal/mouse lowest published toxic dose: 509.58 mg/kgBlood: Methemoglobinemia- CarboxhemoglobinemiaJAPTO* 27,25,2007
intraperitoneal/rat lowest published toxic dose: 510 mg/kgBlood: Methemoglobinemia- CarboxhemoglobinemiaVCVGH* -,320,1990
intraperitoneal/rat lethal dose (50 percent kill): 916 mg/kgENVRAL 40,411,1986
intratracheal/rat lowest published lethal dose: 350 mg/kgLung, Thorax, or Respiration: Structural or functional change in trachea or bronchi

Lung, Thorax, or Respiration: Acute pulmonary edema
NTIS** OTS0520615
intravenous/Dog lowest published lethal dose: 200 mg/kgQJPPAL 7,205,1934
intravenous/mouse lowest published toxic dose: 133 mg/kgKidney, Ureter, and Bladder: Other changesVCVGH* -,320,1990
intravenous/rat lowest published toxic dose: 1000 mg/kgLiver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Cytochrome oxidases (indlucing oxidative phosphrylation)
VCVGH* -,320,1990
oral/Dog lowest published lethal dose: 3 gm/kgQJPPAL 7,205,1934
oral/Dog lethal dose (50 percent kill): 3000 mg/kgVCVGH* -,320,1990
oral/human lowest published lethal dose: 357 mg/kgPeripheral Nerve and Sensation: Paresthesia

Behavioral: Somnolence (general depressed activity)

Behavioral: Convulsions or effect on seizure threshold
34ZIAG -,390,1969
oral/man lowest published toxic dose: 1429 µL/kgKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Blood: Normocytic anemia

Nutritional and Gross Metabolic: Changes in: Fe
YAKUD5 36,575,1994
oral/mouse lethal dose (50 percent kill): 873 mg/kgEVHPAZ 106(Suppl 2),497,1998
oral/mouse lethal dose (16 percent kill): 620 mg/kgVCVGH* -,320,1990
oral/mouse lethal dose (84 percent kill): 1650 mg/kgVCVGH* -,320,1990
oral/rabbit lethal dose (50 percent kill): 2000 mg/kgVCVGH* -,320,1990
oral/rabbit lowest published lethal dose: 1900 mg/kgHBTXAC 1,94,1955
oral/rat lethal dose (50 percent kill): 1600 mg/kgBehavioral: AtaxiaFAONAU 48A,94,1970
oral/rat lethal dose (50 percent kill): 985 mg/kgVCVGH* -,320,1990
oral/rat lethal dose (16 percent kill): 800 mg/kgVCVGH* -,320,1990
oral/rat lethal dose (84 percent kill): 2200 mg/kgVCVGH* -,320,1990
oral/rat lowest published toxic dose: 237.8 mg/kgKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)VCVGH* -,321,1990
oral/rat lowest published toxic dose: 160 mg/kgBlood: Other changesTXAPA9 250,162,2011
subcutaneous/mouse lethal dose (50 percent kill): 6460 mg/kgTXAPA9 4,354,1962
subcutaneous/rabbit lowest published lethal dose: 2700 mg/kgQJPPAL 7,205,1934
unreported route/mouse lethal dose (50 percent kill): 4770 mg/kgGastrointestinal: Ulceration or bleeding from stomach

Gastrointestinal: Ulceration or bleeding from small intestine
ESKGA2 28,P31,1982
unreported route/rabbit lethal dose (50 percent kill): 1225 mg/kgGISAAA 53(6),78,1988
unreported route/rat lethal dose (50 percent kill): 5350 mg/kgGISAAA 53(6),78,1988

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
inhalation/Dog lowest published toxic concentration: 1000 ppm/24H/7W- continuousBehavioral: Food intake (animal)

Musculoskeletal: Other changes

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NTIS** AD746-295
inhalation/Dog lowest published toxic concentration: 5000 ppm/6H/12W- intermittentBehavioral: Hallucinations, distorted perceptions

Behavioral: Ataxia

Behavioral: Antipsychotic
NTIS** OTS0555748
inhalation/Dog lowest published toxic concentration: 34 gm/m3/7W- intermittentBehavioral: General anesthetic

Liver: Fatty liver degeneration
TPKVAL 1,96,1961
inhalation/guinea pig lowest published toxic concentration: 552 ppm/6H/5D- intermittentLiver: Other changes

Biochemical: Metabolism (intermediary): Lipids including transport
AIHAAP 37,345,1976
inhalation/guinea pig lowest published toxic concentration: 34 gm/m3/7W- intermittentBehavioral: General anesthetic

Liver: Fatty liver degeneration
TPKVAL 1,96,1961
inhalation/monkey lowest published toxic concentration: 5000 ppm/24H/32D- continuousBehavioral: Food intake (animal)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NTIS** AD746-295
inhalation/mouse lowest published toxic concentration: 49567.7 mg/kg/13W- intermittentLiver: Other changesMUREAV 746,135,2012
inhalation/mouse lowest published toxic concentration: 1.04 gm/m3/5D- intermittentLung, Thorax, or Respiration: Other changes

Liver: Other changes

Kidney, Ureter, and Bladder: Other changes
TOXID9 66,62,2002
inhalation/mouse lowest published toxic concentration: 13000 ppm/6H/19D- intermittentRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldNTPTR* NTP-TR-306,1986
inhalation/mouse lowest published toxic concentration: 150 ppm/24H/30D- continuousLiver: Changes in liver weight

Endocrine: Changes in spleen weight

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other esterases
APTOA6 59,73,1986
inhalation/mouse lowest published toxic concentration: 5000 ppm/24H/7D- continuousLiver: Changes in liver weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
TXAPA9 23,660,1972
inhalation/mouse lowest published toxic concentration: 300 ppm/24H/90D- continuousLiver: Changes in liver weight

Endocrine: Changes in spleen weight

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other esterases
APTOA6 59,73,1986
inhalation/rat lowest published toxic concentration: 1000 ppm/2H/3W- intermittentBehavioral: Food intake (animal)

Liver: Other changes

Blood: Other changes
HOCHE8 8,64,1990
inhalation/rat lowest published toxic concentration: 1000 ppm/6H/3D- intermittentBrain and Coverings: Other degenerative changes

Biochemical: Neurotransmitters or modulators (putative): Dopamine at other sites
TXCYAC 29,293,1984
inhalation/rat lowest published toxic concentration: 0.045 gm/m3/91D- intermittentBehavioral: Alteration of classical conditioningTPKVAL 1,96,1961
inhalation/rat lowest published toxic concentration: 0.25 gm/m3/24D- intermittentBehavioral: Alteration of classical conditioningTPKVAL 1,96,1961
inhalation/rat lowest published toxic concentration: 3700 ppm/5H/4W- intermittentLung, Thorax, or Respiration: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Phosphatases

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Dehydrogenases
TOLED5 7,41,1980
inhalation/rat lowest published toxic concentration: 5000 ppm/24H/14W- continuousLiver: Other changes

Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Kidney, Ureter, and Bladder: Changes in bladder weight
NTIS** AD746-295
inhalation/rat lowest published toxic concentration: 8400 ppm/6H/13W- intermittentLiver: Changes in liver weight

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NTPTR* NTP-TR-306,1986
inhalation/rat lowest published toxic concentration: 500 ppm/6H/2W- intermittentKidney, Ureter, and Bladder: Other changes

Blood: Methemoglobinemia- Carboxhemoglobinemia

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other enzymes
RCOCB8 32,535,1981
inhalation/rat lowest published toxic concentration: 2800 mg/m3/4D- intermittentPeripheral Nerve and Sensation: Recording from peripheral motor nerve

Liver: Liver function tests impaired

Kidney, Ureter, and Bladder: Other changes
TPKVAL 15,76,1979
inhalation/rat lowest published toxic concentration: 2800 mg/m3/8D- intermittentKidney, Ureter, and Bladder: Other changes in urine compositionTPKVAL 15,76,1979
inhalation/rat lowest published toxic concentration: 2800 mg/m3/27D- intermittentNutritional and Gross Metabolic: Weight loss or decreased weight gainTPKVAL 15,76,1979
inhalation/rat lowest published toxic concentration: 880 mg/m3/4D- intermittentPeripheral Nerve and Sensation: Recording from peripheral motor nerveTPKVAL 15,76,1979
inhalation/rat lowest published toxic concentration: 880 mg/m3/15D- intermittentKidney, Ureter, and Bladder: Other changes in urine compositionTPKVAL 15,76,1979
inhalation/rat lowest published toxic concentration: 0.4 gm/m3/91D- intermittentBrain and Coverings: Other degenerative changesTPKVAL 1,72,1961
inhalation/rat lowest published toxic concentration: 5000 ppm/28D- intermittentLiver: Changes in liver weight

Endocrine: Differential effect of sex or castration on observed toxicity

Endocrine: Changes in spleen weight
JTEHF8 66,1207,2003
oral/mouse lowest published toxic dose: 1862 mg/kg/2W- intermittentKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)DCTODJ 6,563,1983
oral/mouse lowest published toxic dose: 52740 mg/kg/90D- continuousLiver: Hepatitis (hepatocellular necrosis), zonal

Liver: Fatty liver degeneration
FCTOD7 24,943,1986
oral/rat lowest published toxic dose: 14940 mg/kg/90D- continuousLiver: Hepatitis (hepatocellular necrosis), zonal

Liver: Fatty liver degeneration
FCTOD7 24,943,1986
oral/rat lowest published toxic dose: 91 gm/kg/2Y- continuousBehavioral: Fluid intake

Liver: Fatty liver degeneration

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
FCTOD7 24,951,1986
skin/rat lowest published toxic dose: 39270 mg/kg/17W- intermittentLiver: Other changes

Liver: Changes in liver weight
GTPZAB 26(5),41,1982

Reviews

OrganizationStandardReference
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-time-weighted average 50 ppmDTLVS* TLV/BEI,2013
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-Confirmed animal carcinogenDTLVS* TLV/BEI,2013
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 41,43,1986
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 71,251,1999
International Agency for Research on Cancer (IARC)Cancer Review:Human Inadequate EvidenceIMEMDT 41,43,1986
International Agency for Research on Cancer (IARC)Cancer Review:Human Inadequate EvidenceIMEMDT 71,251,1999
International Agency for Research on Cancer (IARC)Cancer Review:Group 2BIMEMDT 71,251,1999
TOXICOLOGY REVIEWFAZMAE 18,365,1974
TOXICOLOGY REVIEWTOLED5 127,135,2002
TOXICOLOGY REVIEWTOLED5 138,9,2003
TOXICOLOGY REVIEWMUREAV 543,201,2003
TOXICOLOGY REVIEWMUREAV 567,227,2004
TOXICOLOGY REVIEWMUREAV 567,109,2004
TOXICOLOGY REVIEWMUREAV 567,151,2004
TOXICOLOGY REVIEWMUREAV 567,347,2004
TOXICOLOGY REVIEWEMMUEG 39,69,2002
TOXICOLOGY REVIEWDIMON* 46,551,2000
TOXICOLOGY REVIEWDIMON* 39,678,1993
TOXICOLOGY REVIEWMUREAV 628,67,2007
TOXICOLOGY REVIEWENTOX* -,92,2005
TOXICOLOGY REVIEWMUREAV 584,1,2005
TOXICOLOGY REVIEWBCLPT* 96,131,2005
TOXICOLOGY REVIEWMUREAV 636,242,2007
TOXICOLOGY REVIEWMUREAV 654,114,2008
TOXICOLOGY REVIEWHUTOX* -,661,1996
TOXICOLOGY REVIEWHUTOX* -,683,1996
TOXICOLOGY REVIEWHUTOX* -,577,1996
TOXICOLOGY REVIEWHTOPA* 1,501,2002
TOXICOLOGY REVIEWTOLED5 180,100,2008
TOXICOLOGY REVIEWAIRMJ* 20,8,2001
TOXICOLOGY REVIEWMUREAV 682,39,2009
TOXICOLOGY REVIEWNCLNA* 16,503,1998
TOXICOLOGY REVIEWNTAPM* -,629,1995
TOXICOLOGY REVIEWNTAPM* -,711,1995
TOXICOLOGY REVIEWNTAPM* -,727,1995
TOXICOLOGY REVIEWMUREAV 705,184,2010
TOXICOLOGY REVIEWMUREAV 751,49,2012
TOXICOLOGY REVIEWMUREAV 752,99,2013
TOXICOLOGY REVIEWTXAPA9 254,100,2011
TOXICOLOGY REVIEWTXAPA9 255,113,2011
TOXICOLOGY REVIEWMUREAV 769,34,2014
TOXICOLOGY REVIEWMUREAV 762,76,2014
TOXICOLOGY REVIEWFCTOD7 55,596,2013
TOXICOLOGY REVIEWTXAPA9 266,345,2013
TOXICOLOGY REVIEWMUREAV 802,1,2016
TOXICOLOGY REVIEWMUREAV 770,92,I2

Standards and Regulations

OrganizationStandardReference
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATIONFEREAC 54,7740,1989
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1998 STATUS OF PESTICIDESCancelledRBREV* -,263,1998
Mine Safety and Health Administration (MSHA) STANDARD-airtime-weighted average 500 ppm (1750 mg/m3)DTLVS* 3,171,1971
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV;
Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV
Occupational Exposure Limit-AUSTRALIAtime-weighted average 50 ppm (174 mg/m3), Carcinogen, JUL2008
Occupational Exposure Limit-AUSTRIAMAK-TMW 50 ppm (175 mg/m3);KZW 200 ppm (700 mg/m3), skin, 2007
Occupational Exposure Limit-BELGIUMtime-weighted average 50 ppm (177 mg/m3), MAR2002
Occupational Exposure Limit-DENMARKtime-weighted average 35 ppm (122 mg/m3), skin, carc, MAY2011
Occupational Exposure Limit-FINLANDtime-weighted average 100 ppm (350 mg/m3), short term exposure limit 250 ppm (880 mg/m3), NOV2011
Occupational Exposure Limit-FRANCEVME 50 ppm (180 mg/m3), VLE 100 ppm, C3 Carcinogen, FEB2006
Occupational Exposure Limit-HUNGARYtime-weighted average 10 mg/m3, short term exposure limit 10 mg/m3, SEP2000
Occupational Exposure Limit-ICELANDtime-weighted average 35 ppm (122 mg/m3), skin, NOV2011
Occupational Exposure Limit-JAPANOccupational Exposure Limit 50 ppm (170 mg/m3), Occupational Exposure Limit-continuous 100 ppm (340 mg/m3), skin, 2B carc, MAY2012
Occupational Exposure Limit-KOREAtime-weighted average 50 ppm (175 mg/m3), 2006
Occupational Exposure Limit-MEXICOtime-weighted average 100 ppm (330 mg/m3);short term exposure limit 500 ppm (1740 mg/m3), 2004
Occupational Exposure Limit-NEW ZEALANDtime-weighted average 50 ppm (174 mg/m3), JAN2002
Occupational Exposure Limit-NORWAYtime-weighted average 35 ppm (125 mg/m3), JAN1999
Occupational Exposure Limit-PERUtime-weighted average 50 ppm (174 mg/m3), JUL2005
Occupational Exposure Limit-POLANDMAC(time-weighted average) 20 mg/m3, short term exposure limit 50 mg/m3, JAN1999
Occupational Exposure Limit-RUSSIAtime-weighted average 50 mg/m3, short term exposure limit 100 mg/m3, JUN2003
Occupational Exposure Limit-SWEDENtime-weighted average 35 ppm (120 mg/m3);short term exposure limit 70 ppm (250 mg/m3), Skin, Carcinogen, JUN2005
Occupational Exposure Limit-SWITZERLANDMAK-week 50 ppm (180 mg/m3), carc 3, JAN2011
Occupational Exposure Limit-THAILANDtime-weighted average 500 mg/m3, short term exposure limit 1000 mg/m3, JAN1993
Occupational Exposure Limit-THE NETHERLANDSMAC-TGG 350 mg/m3, 2003
Occupational Exposure Limit-THE PHILIPPINEStime-weighted average 500 ppm (1740 mg/m3), JAN1993
Occupational Exposure Limit-TURKEYtime-weighted average 500 ppm (1740 mg/m3), JAN1993
Occupational Exposure Limit-UNITED KINGDOMtime-weighted average 100 ppm (350 mg/m3);short term exposure limit 300 ppm (1060 mg/m3), skin, OCT2007
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction)see 56 FR 57036CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors)8H time-weighted average 500 ppm (1740 mg/m3)CFRGBR 41,50-204.50,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry)8H time-weighted average 500 ppm;ceiling concentration 1000 ppm;Pk 2000 ppm/5M/2HCFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards)8H time-weighted average 500 ppm (1740 mg/m3CFRGBR 29,1915.1000,1993

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO METHYLENE CHLORIDE-airCarcinogen lowest feasible concentrationNIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983Hazard Code 47270; Number of Industries 363; Total Number of Facilities 87086; Number of Occupations 212; Total Number of Employees Exposed 1438196; Total Number of Female Employees Exposed 352536
National Occupational Hazard Survey 1974Hazard Code 47270; Number of Industries 374; Total Number of Facilities 89025; Number of Occupations 192; Total Number of Employees Exposed 975696

Status in Federal Agencies

OrganizationReference
ATSDR TOXICOLOGY PROFILE (NTIS** PB/2000/108026)
EPA GENETOX PROGRAM 1988, Negative: D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Positive: Cell transform.-RLV F344 rat embryo
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: S cerevisiae gene conversion; S cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Positive: S cerevisiae-reversion
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULEFEREAC 47,26992,82
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NCI Carcinogenesis Studies (inhal);clear evidence:mouse,rat
NIOSH Analytical Method, 1994: Methylene chloride, 1005
NIOSH Analytical Method, 1996: Volatile organic compound, 2549
NIOSH CURRENT INTELLIGENCE BULLETIN 46, 1986
NTP 11th Report on Carcinogens,2004:Reasonably anticipated to be a human carcinogen
On EPA IRIS database
OSHA ANALYTICAL METHOD #80
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