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Registry of Toxic Effects of Chemical Substances (RTECS)

Hydrazine, methyl-

RTECS #

MV5600000

CAS #

60-34-4

Updated

September 2013

Molecular Weight

46.09

Molecular Formula

CH6N2

Synonyms

1-Methylhydrazine
Methyl hydrazine
Methylhydrazine (ACGIH:OSHA)
Metylohydrazyna (Polish)
MMH
Monomethylhydrazine (OSHA)
RCRA waste number P068

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
DNA adductfibroblast/human116 pmolNTIS** AD-A092-249
DNA inhibitionAscites tumor/mouse581 µmol/LJJATDK 1,243,1981
Dominant Lethal Testintraperitoneal/rat1075 mg/kg/5D- intermittentNTIS** AD-A041-973
mutation in microorganisms/Salmonella typhimurium10 µmol/plate (+enzymatic activation step)MUREAV 54,167,1978
mutation in microorganisms/Salmonella typhimurium1 µL/plate (-enzymatic activation step)NTIS** AD-A041-973
mutation in microorganisms/Escherichia coli500 nmol/disc (-enzymatic activation step)MUREAV 71,269,1980
other mutation test systemsAscites tumor/mouse758 µmol/LJJATDK 1,243,1981
Unscheduled DNA Synthesisovary/hamster1 µmol/LNTIS** AD-A075-605
Unscheduled DNA Synthesislung/hamster1 µmol/LNTIS** AD-A075-605
Unscheduled DNA Synthesislung/rat1 µmol/LNTIS** AD-A075-605

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
intraperitoneal/rat 100 mg/kg (6-15D pregnant)Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants)APTOD9 19,A21,1980
intraperitoneal/rat 50 mg/kg (6-15D pregnant)Reproductive: Specific developmental abnormalities: Eye, earAPTOD9 19,A21,1980
intravenous/rat 24 mg/kg (6-13D pregnant)Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants)

Reproductive: Other effects to embryo or fetus
FACOEB 10,391,1993
intravenous/rat 33600 µg/kg (6-13D pregnant)Reproductive: Effects on fertility: Female fertility index (e.g., # females pregnant per # sperm positive females; # females pregnant per # females mated)FACOEB 10,391,1993
oral/rat 5 mg/kg (6D pregnant)Reproductive: Effects on fertility: Pre- implantation mortality (e.g., reduction in number of implants per female; total number of implants per corpora lutea)FACOEB 10,391,1993

Tumorigenic Data and References

Route/OrganismDoseEffectReference
inhalation/hamster lowest published toxic concentration: 5 ppm/6H/1Y- intermittentTumorigenic: Neoplastic by RTECS criteria

Olfaction: Tumors

Endocrine: Adrenal cortex hyperplasia
NTIS** AD-A154-659
inhalation/mouse lowest published toxic concentration: 2 ppm/6H/1Y- intermittentTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
NTIS** AD-A154-659
inhalation/rat lowest published toxic concentration: 20 ppb/6H/1Y- intermittentTumorigenic: Carcinogenic by RTECS criteria

Blood: Leukemia
NTIS** AD-A154-659
intraperitoneal/mouse lowest published toxic dose: 72 mg/kg/8W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Lung, Thorax, or Respiration: Tumors
JNCIAM 42,337,1969
oral/hamster lowest published toxic dose: 3000 mg/kg/47W- continuousTumorigenic: Carcinogenic by RTECS criteria

Gastrointestinal: Colon tumors

Liver: Tumors
CNREA8 33,2744,1973
oral/hamster toxic dose: 5768 mg/kg/2Y- continuousTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Liver: Tumors
AJPAA4 70,102,1973
oral/mouse lowest published toxic dose: 10 gm/kg/1Y- continuousTumorigenic: Neoplastic by RTECS criteria

Lung, Thorax, or Respiration: Tumors

Blood: Lymphoma including Hodgkin's disease
IJCNAW 9,109,1972

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
inhalation/Dog lethal concentration (50 percent kill): 96 ppm/1HAIHAAP 31,667,1970
inhalation/guinea pig lethal concentration (50 percent kill): 270 mg/m3/4HBrain and Coverings: Other degenerative changes

Lung, Thorax, or Respiration: Acute pulmonary edema

Liver: Liver function tests impaired
STGNBT -,163,1999
inhalation/hamster lethal concentration (50 percent kill): 143 ppm/4HBehavioral: Convulsions or effect on seizure thresholdAMIHAB 12,609,1955
inhalation/monkey Lowest published lethal concentration: 16 ppm/10MTOXID9 44,303,2005
inhalation/monkey Lowest published lethal concentration: 5.5 ppm/30MTOXID9 44,303,2005
inhalation/monkey Lowest published lethal concentration: 2.7 ppm/1HTOXID9 44,303,2005
inhalation/monkey Lowest published lethal concentration: 0.68 ppm/4HTOXID9 44,303,2005
inhalation/monkey Lowest published lethal concentration: 0.34 ppm/8HTOXID9 44,303,2005
inhalation/monkey lethal concentration (50 percent kill): 82 ppm/1HEye: Conjunctiva irritation

Gastrointestinal: Nausea or vomiting

Behavioral: Convulsions or effect on seizure threshold
AIHAAP 31,667,1970
inhalation/mouse lethal concentration (50 percent kill): 56 ppm/4HEye: Other eye effects

Behavioral: Convulsions or effect on seizure threshold

Lung, Thorax, or Respiration: Dyspnea
AMIHAB 12,609,1955
inhalation/rat lethal concentration (50 percent kill): 34 ppm/4HAMRL** TR-67-137,1967
intraperitoneal/hamster lethal dose (50 percent kill): 21 mg/kgCTOXAO 4,435,1971
intraperitoneal/mouse lethal dose (50 percent kill): 15 mg/kgEye: Miosis (pupilliary constriction)

Behavioral: Convulsions or effect on seizure threshold

Skin and Appendages: Other: Hair
PSEBAA 124,172,1967
intraperitoneal/rat lethal dose (50 percent kill): 21 mg/kgCTOXAO 4,435,1971
intravenous/Dog lethal dose (50 percent kill): 12 mg/kgBlood: Methemoglobinemia- CarboxhemoglobinemiaPSEBAA 131,226,1969
intravenous/mouse lethal dose (50 percent kill): 33200 µg/kgMEPAAX 24,71,1973
intravenous/rabbit lethal dose (50 percent kill): 12 mg/kgBlood: Methemoglobinemia- CarboxhemoglobinemiaPSEBAA 131,226,1969
intravenous/rat lethal dose (50 percent kill): 17 mg/kgCTOXAO 4,435,1971
oral/hamster lethal dose (50 percent kill): 22 mg/kgCTOXAO 4,435,1971
oral/mouse lethal dose (50 percent kill): 29 mg/kgBehavioral: Somnolence (general depressed activity)

Behavioral: Convulsions or effect on seizure threshold

Behavioral: Excitement
NTIS** AD-A125-539
oral/rat lethal dose (50 percent kill): 32 mg/kgBehavioral: Convulsions or effect on seizure thresholdXAWPA2 CWL 2-10,1958
skin/guinea pig lethal dose (50 percent kill): 48 mg/kgBlood: Methemoglobinemia- CarboxhemoglobinemiaPSEBAA 131,226,1969
skin/hamster lethal dose (50 percent kill): 239 mg/kgCTOXAO 4,435,1971
skin/rabbit lethal dose (50 percent kill): 95 mg/kgBlood: Methemoglobinemia- CarboxhemoglobinemiaPSEBAA 131,226,1969
skin/rat lethal dose (50 percent kill): 183 mg/kgCTOXAO 4,435,1971
subcutaneous/mouse lethal dose (50 percent kill): 25 mg/kgBJCAAI 30,429,1974
subcutaneous/rat lethal dose (50 percent kill): 35 mg/kgBJCAAI 30,429,1974

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
inhalation/Dog lowest published toxic concentration: 200 ppb/24H/30W- continuousBlood: Methemoglobinemia- Carboxhemoglobinemia

Nutritional and Gross Metabolic: Changes in: P

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Phosphatases
NTIS** AD734-543
inhalation/Dog lowest published toxic concentration: 200 ppb/24H/26W- continuousBlood: Changes in bone marrow not included in above

Blood: Changes in erythrocyte (RBC) count

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Phosphatases
NTIS** AD-751440
inhalation/monkey lowest published toxic concentration: 5 ppm/6H/26W- intermittentBlood: Other changesNTIS** AD-751440
inhalation/mouse lowest published toxic concentration: 5 ppm/6H/26W- intermittentRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldNTIS** AD-751440
inhalation/rat lowest published toxic concentration: 200 ppb/24H/26W- continuousNutritional and Gross Metabolic: Weight loss or decreased weight gainNTIS** AD-751440

Reviews

OrganizationStandardReference
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-time-weighted average 0.01 ppm (skin)DTLVS* TLV/BEI,2013
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-Confirmed animal carcinogenDTLVS* TLV/BEI,2013
TOXICOLOGY REVIEWMUREAV 39,111,1977
TOXICOLOGY REVIEWARVPAX 5,447,1965
TOXICOLOGY REVIEWJEMME* 28,175,2005
TOXICOLOGY REVIEWENTOX* -,171,2005
TOXICOLOGY REVIEWMUREAV 584,1,2005

Standards and Regulations

OrganizationStandardReference
Mine Safety and Health Administration (MSHA) STANDARDair-ceiling concentration 0.2 ppm (0.35 mg/m3) (skin)DTLVS* 3,174,1971
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV;
Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV
Occupational Exposure Limit-AUSTRALIAtime-weighted average 0.01 ppm (0.019 mg/m3), Carcinogen, JUL2008
Occupational Exposure Limit-BELGIUMtime-weighted average 0.01 ppm (0.019 mg/m3), Skin, MAR2002
Occupational Exposure Limit-DENMARKtime-weighted average 0.01 ppm (0.02 mg/m3), skin, MAY2011
Occupational Exposure Limit-FINLANDtime-weighted average 0.01 ppm (0.02 mg/m3), skin, NOV2011
Occupational Exposure Limit-FRANCEVME 0.2 ppm (0.35 mg/m3), FEB2006
Occupational Exposure Limit-ICELANDtime-weighted average 0.04 ppm (0.08 mg/m3), skin, NOV2011
Occupational Exposure Limit-KOREAceiling concentration 0.2 ppm (0.35 mg/m3), skin, 2006
Occupational Exposure Limit-MEXICOtime-weighted average 0.01 ppm (0.019 mg/m3), 2004
Occupational Exposure Limit-NEW ZEALANDceiling concentration 0.2 ppm (0.38 mg/m3), skin, JAN2002
Occupational Exposure Limit-NORWAYtime-weighted average 0.08 mg/m3, JAN1999
Occupational Exposure Limit-PERUtime-weighted average 0,01 ppm (0,019 mg/m3), JUL2005
Occupational Exposure Limit-POLANDMAC(time-weighted average) 0.02 mg/m3, MAC(short term exposure limit) 0.1 mg/m3, JAN1999
Occupational Exposure Limit-SWEDENGroup B Carcinogen, JUN2005
Occupational Exposure Limit-SWITZERLANDMAK-week 0.2 ppm (0.35 mg/m3), skin, JAN2011
Occupational Exposure Limit-THAILANDtime-weighted average 0.2 ppm (0.35 mg/m3), JAN1993
Occupational Exposure Limit-THE NETHERLANDSMAC-C 0.35 mg/m3, Skin, 2003
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction)ceiling concentration 0.2 ppm (0.35 mg/m3) (skin)CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors)ceiling concentration 0.2 ppm (0.35 mg/m3) (skin)CFRGBR 41,50-204.50,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry)ceiling concentration 0.2 ppm (0.35 mg/m3) (skin)CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards)ceiling concentration 0.2 ppm (0.35 mg/m3) (skin)CFRGBR 29,1915.1000,1993

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO METHYLHYDRAZINE-airCarcinogen ceiling concentration 0.04 ppm/120MNIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983Hazard Code X4272; Number of Industries 1; Total Number of Facilities 14; Number of Occupations 2; Total Number of Employees Exposed 1473; Total Number of Female Employees Exposed 230

Status in Federal Agencies

OrganizationReference
EPA GENETOX PROGRAM 1988, Negative: Rodent dominant lethal; In vitro UDS-human fibroblast
EPA GENETOX PROGRAM 1988, Negative: S cerevisiae gene conversion
EPA GENETOX PROGRAM 1988, Positive/limited: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test; TRP reversion
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NIOSH Analytical Method, 1994: Monomethylhydrazine, 3510
On EPA IRIS database
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