Skip directly to search Skip directly to A to Z list Skip directly to navigation Skip directly to page options Skip directly to site content

Registry of Toxic Effects of Chemical Substances (RTECS)

Acetamide, N-fluoren-2-yl-

RTECS #

AB9450000

CAS #

53-96-3

Updated

April 2017

Molecular Weight

223.29

Molecular Formula

C15H13NO

Synonyms

2-(Acetylamino)fluorene
2AAF
2-AAF
2-Acetamidofluorene
2-Acetaminofluorene
2-Acetylamino-fluoren (German)
2-Acetylaminofluorene
2-Acetylaminofluorene (OSHA)
2-FAA
2-Fluorenylacetamide
AAF
Acetamide, N-9H-fluoren-2-yl- (9CI)
Acetoaminofluorene
Acetylaminofluorene
Azetylaminofluoren (German)
FAA
N-2-Fluorenylacetamide
N-9H-Fluoren-2-ylacetamide
N-Acetyl-2-aminofluorene
N-Fluoren-2-ylacetamide
RCRA waste number U005

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
body fluid assaySalmonella typhimurium/rat1600 µg/kgPNASA6 71,737,1974
body fluid assayovary/rat53 mg/kgMUREAV 143,263,1985
body fluid assaySalmonella typhimurium/mouse20 mg/kgMUREAV 117,79,1983
Cytogenetic Analysisoral/rat50 mg/kgMUREAV 251,59,1991
Cytogenetic Analysisliver/rat10 mg/LMUREAV 64,329,1979
Cytogenetic Analysislung/hamster10 mg/LATSUDG 4,41,1980
Cytogenetic Analysislung/hamster20 mg/L/2HMUREAV 604,8,2006
DNA adductunreported route/rat93.8 gm/kg/12W- intermittentTOXID9 66,181,2002
DNA adductoral/rat1.24 mg/kg/16W- intermittentTOXID9 78,224,2004
DNA adductoral/mouse31.36 mg/kg/31D- intermittentTOXID9 -,454,2009
DNA adductlymphocyte/wild bird700 pmol/plateCNREA8 37,3756,1977
DNA adductfibroblast/human50 µmol/L/2HMUREAV 484,3,2001
DNA adductintraperitoneal/rat50 mg/kgMUTAEX 17,45,2002
DNA adductliver/mouse60 µmol/LJJIND8 62,947,1979
DNA adductAscites tumor/mouse5 µmol/LMUREAV 89,95,1981
DNA adduct/Escherichia coli5 µmol/LMUREAV 89,95,1981
DNA adductHeLa cell/human5 µmol/LMUREAV 89,95,1981
DNA adductother cell types/human1200 nmol/LPAACA3 24,63,1983
DNA adductintraperitoneal/rat18 µmol/kgCBINA8 48,15,1984
DNA adductoral/rat70 mg/kgCRNGDP 15,857,1994
DNA adductoral/rat0.924 mg/kg/12W- intermittentMUTAEX 26,605,2011
DNA Damageoral/rat2.016 gm/kg/24W- intermittentMUREAV 722,106,2011
DNA Damageother cell types/mouse0.125 mmol/LMUREAV 715,25,2011
DNA Damageother cell types/human10 µmol/L/24H (+/-enzymatic activation step)MUREAV 760,23,2014
DNA Damageoral/rat750 mg/kg/3D- intermittentMUREAV 786-788,172,2015
DNA Damageother cell types/human100 µmol/L/24HMUTAEX 31,83,2016
DNA Damageliver/rat2000 ppmENMUDM 4,667,1982
DNA Damagefibroblast/human1 mmol/LENMUDM 7,267,1985
DNA Damageother cell types/human50 µmol/LMUREAV 468,277,2000
DNA Damage/Bacillus subtilis1 mg/discPMRSDJ 1,175,1981
DNA Damageliver/rat3 µmol/LSinJF# 26OCT1982
DNA Damageliver/rat50 µg/LENMUDM 6,427,1984
DNA Damageoral/other insects0.25 µmol/tubeMUREAV 480-481,139,2001
DNA Damageintraperitoneal/mouse300 mg/kgMUREAV 493,39,2001
DNA Damageoral/mouse600 mg/kgMUREAV 493,39,2001
DNA Damagelung/hamster300 µmol/L/2HBBRCA9 72,732,1976
DNA Damageoral/rat504 mg/kg/6W-continuousTOXID9 12,402,2008
DNA Damageoral/rat75 mg/kgMUTAEX 23,233,2008
DNA Damageother cell types/human0.1 mmol/l/48HMUREAV 696,21,2010
DNA Damageliver/human31.6 µmol/l/48HMUREAV 696,21,2010
DNA Damageliver/human1 mmol/l/48HMUREAV 696,21,2010
DNA Damageliver/mouse125 µmol/L/24HMUTAEX 25,561,2010
DNA Damageliver/mouse125 µmol/L/48HMUTAEX 25,561,2010
DNA Damageintraperitoneal/rat270 mg/kg/3D- intermittentMUREAV 722,7,2011
DNA Damagelung/hamster20 mg/LMUREAV 604,8,2006
DNA Damageliver/rat100 µmol/L/12HMUREAV 519,163,2002
DNA Damageliver/human150 µmol/L/12HMUREAV 519,163,2002
DNA Damagelung/hamster0.1 mmol/L/24HMUREAV 521,57,2002
DNA inhibitionliver/rat100 nmol/LCNREA8 45,337,1985
DNA inhibitionoral/rat420 mg/kg/6WCNREA8 33,397,1973
DNA inhibitionoral/mouse160 mg/kgCBINA8 5,153,1972
DNA inhibitionintraperitoneal/mouse200 mg/kgMUREAV 46,305,1977
DNA repairoral/Drosophila melanogaster500 mg/LMUREAV 290,175,1993
DNA repair/Escherichia coli10 mg/LJJIND8 62,873,1979
DNA repair/Schizosaccharomyces pombe2800 nmol/LCNJGA8 24,771,1982
DNA repairliver/rat1 µmol/LJJCREP 79,204,1988
DNA repairovary/hamster6.25 µmol/L/18HMUREAV 564,139,2004
DNA repairoral/rat504 mg/kg/6W-continuousTOXID9 12,402,2008
gene conversion and mitotic recombination/Saccharomyes cerevisiae1 gm/LPMRSDJ 1,434,1981
host-mediated assaySalmonella typhimurium/mouse495 mg/kgJJIND8 62,911,1979
host-mediated assaySalmonella typhimurium/rat90 mg/kg/5DAJCNAC 30,1921,1977
micronucleus testintraperitoneal/mouse2 mg/kgPMRSDJ 1,686,1981
micronucleus testoral/mouse75 mg/kgMUREAV 223,361,1989
micronucleus testoral/rat50 mg/kgMUREAV 251,59,1991
micronucleus testother cell types/human0.17 µmol/L/20HMUREAV 536,79,2003
micronucleus testother cell types/mouse31.25 mg/L/4H (+enzymatic activation step)MUTAEX 14,403,1999
micronucleus testunreported route/rat6720 mg/kg/28DMUREAV 680,31,2009
micronucleus testovary/hamster333 µmol/L/24H (-enzymatic activation step)MUREAV 630,1,2007
micronucleus testovary/hamster100 µmol/L/3H (+enzymatic activation step)MUREAV 724,7,2011
micronucleus testliver/human0.17 µmol/L/20HMUREAV 536,79,2003
micronucleus testmammary gland/mouse28 mg/L/3H (+enzymatic activation step)MUREAV 540,153,2003
micronucleus testintraperitoneal/hamster2 gm/kgMUREAV 43,255,1977
micronucleus testembryo/hamster1 µmol/LMUREAV 203,397,1988
micronucleus testlung/hamster100 mg/LMUREAV 468,137,2000
micronucleus testoral/rat1680 mg/kg/28DMUREAV 780-781,2,2015
micronucleus testother cell types/human160 mg/L/3H (+enzymatic activation step)MUREAV 767,28,2014
micronucleus testlymphocyte/human41.2 mg/L/3H (+enzymatic activation step)MUREAV 767,28,2014
morphological transformfibroblast/mouse10 mg/L/48HMUREAV 744,20,2012
morphological transformembryo/mouse20 mg/L/72HMUREAV 702,100,2010
morphological transformembryo/mouse5 mg/L/10DMUREAV 702,100,2010
morphological transformoral/rat232.96 mg/kg/104W- intermittentMUTAEX 26,605,2011
morphological transformintraperitoneal/hamster750 mg/kgARPAAQ 95,380,1973
morphological transformkidney/hamster80 µg/LBJCAAI 37,873,1978
morphological transformembryo/hamster12500 µg/LJJIND8 67,1303,1981
morphological transformintraperitoneal/rat200 mg/kgCRNGDP 10,435,1989
morphological transformoral/rat480 mg/kg/8W- intermittentCRNGDP 5,171,1984
morphological transformembryo/rat100 µg/LJJIND8 67,1303,1981
morphological transformliver/rat100 µmol/LCNREA8 43,5087,1983
morphological transformmammary gland/mouse1 µg/LCNREA8 39,1784,1979
mutation in mammalian somatic cellsliver/rat1 µmol/LMUREAV 130,53,1984
mutation in mammalian somatic cellslymphocyte/human25 µmol/LENMUDM 4,304,1982
mutation in mammalian somatic cellslymphocyte/mouse15 mg/LMUREAV 125,291,1984
mutation in mammalian somatic cellsoral/rat360 mg/kg/30D-continuousCALEDQ 143,249,1999
mutation in microorganismslymphocyte/mouse12300 µg/L (+enzymatic activation step)EMMUEG 12(Suppl 13),37,1988
mutation in microorganisms/Salmonella typhimurium15 µg/plate (+enzymatic activation step)MUREAV 498,107,2001
mutation in microorganisms/Escherichia coli64 mg/L/16HMUREAV 493,127,2001
mutation in microorganisms/Salmonella typhimurium1 nmol/plate/20MEMMUEG 38,268,2001
mutation in microorganismsovary/hamster20 mg/L (+enzymatic activation step)MUREAV 112,329,1983
mutation in microorganisms/Salmonella typhimurium4 mg/L/72H (+enzymatic activation step)MUREAV 558,181,2004
mutation in microorganisms/Salmonella typhimurium100 µg/plate/72HMUREAV 558,145,2004
mutation in microorganismsmammary gland/Salmonella typhimurium50 µg/plate/24H (+enzymatic activation step)EMMUEG 41,55,2002
mutation in microorganisms/Salmonella typhimurium0.05 mg/plate/45M (+enzymatic activation step)TCMUD8 22,285,2002
mutation in microorganisms/Salmonella typhimurium50 µg/plate/72H (+enzymatic activation step)MUTAEX 14,323,1999
mutation in microorganisms/Salmonella typhimurium2 µmol/L/16H (+enzymatic activation step)EMMUEG 46,126,2005
mutation in microorganisms/Salmonella typhimurium1.1 µmol/L/48HEMMUEG 46,126,2005
mutation in microorganisms/Salmonella typhimurium2.5 µg/plate/20M (+enzymatic activation step)MUTAEX 20,217,2005
mutation in microorganisms/Salmonella typhimurium1 µg/plateMUREAV 577S,1,2005
mutation in microorganisms/Salmonella typhimurium10 µg/plate/48HEMMUEG 47,95,2006
mutation in microorganisms/Salmonella typhimurium2.5 µg/plate/48H (+enzymatic activation step)MUTAEX 20,217,2005
mutation in microorganisms/Saccharomyes cerevisiae313 mg/L/18H (+enzymatic activation step)MUREAV 653,63,2008
mutation in microorganisms/Saccharomyes cerevisiae100 mg/L (+enzymatic activation step)CPBTAL 33,1576,1985
mutation in microorganisms/Schizosaccharomyces pombe100 µg/L (+/-enzymatic activation step)PMRSDJ 1,424,1981
mutation in microorganisms/Other microorganisms200 µg/plate (+enzymatic activation step)CBINA8 22,297,1978
mutation in microorganisms/Salmonella typhimurium300 ng/plate (-enzymatic activation step)ENMUDM 6(Suppl 2),1,1984
mutation in microorganisms/Escherichia coli100 µg/plate (+enzymatic activation step)PMRSDJ 1,387,1981
mutation in microorganismslymphocyte/mouse40 mg/L (+enzymatic activation step)ENMUDM 7(Suppl 3),10,1985
other mutation test systemsoral/mouse160 mg/kgCBINA8 5,153,1972
other mutation test systemsoral/rat72 mg/kg/3D-continuousEXMPA6 31,333,1979
other mutation test systemsoral/rat420 mg/kg/6WCNREA8 33,397,1973
other mutation test systems/Escherichia coli100 µmol/LMUREAV 203,81,1988
other mutation test systems/Salmonella typhimurium50 mg/LMUREAV 192,239,1987
other mutation test systemsintraperitoneal/mouse300 mg/kgMUREAV 747,164,2012
other mutation test systemsother cell types/human3.9 µmol/L/24HTIVIEQ 28,46,2014
phage inhibition capacity/Escherichia coli200 mg/LPMRSDJ 1,224,1981
sex chromosome loss and nondisjunctionparenteral/silkworm500 µgKIKNAJ (28),71,1977
sex chromosome loss and nondisjunction/Saccharomyes cerevisiae50 mg/LPMRSDJ 1,468,1981
sex chromosome loss and nondisjunctionoral/rat50 mg/kgCRNGDP 17,1051,1996
sex chromosome loss and nondisjunctionliver/hamster100 mg/LPMRSDJ 5,397,1985
sister chromatid exchangeintraperitoneal/hamster50 mg/kgMUREAV 113,33,1983
sister chromatid exchangeovary/hamster100 µmol/L/150MMUREAV 58,103,1978
sister chromatid exchangeoral/hamster50 mg/kgMUREAV 113,33,1983
sister chromatid exchangeliver/hamster6 mg/LMUREAV 207,69,1988
sister chromatid exchangeintraperitoneal/rabbit3 mg/kgMUREAV 58,321,1978
sister chromatid exchangeparenteral/chicken3125 ng/kgENMUDM 2,435,1980
sister chromatid exchangeintraperitoneal/mouse20 mg/kgMUREAV 157,181,1985
sister chromatid exchangeintraperitoneal/rat100 mg/kgMUREAV 91,363,1981
sister chromatid exchangeskin/rat100 mg/kgMUREAV 91,363,1981
sister chromatid exchangeliver/rat4500 µmol/LMUREAV 93,409,1982
sister chromatid exchangeother cell types/rat400 µmol/LMUREAV 93,409,1982
sister chromatid exchangeoral/rat25 mg/kgMUREAV 251,59,1991
sister chromatid exchangelymphocyte/human4400 µg/LCNREA8 40,4775,1980
specific locus testoral/other insects1000 ppmMUREAV 268,155,1992
specific locus testparenteral/Drosophila melanogaster20 mmol/LIJCNAW 9,284,1972
specific locus testintraperitoneal/mouse223 mg/kgMUREAV 117,201,1983
specific locus testoral/rat6 mg/kg/4D-continuousCALEDQ 135,215,1999
specific locus testoral/Drosophila melanogaster1 mmol/L/24HMUREAV 514,87,2002
specific locus testovary/hamster5 mg/L/9DEMMUEG 16,260,1990
specific locus testlung/hamster1 µmol/L/24HMUREAV 521,57,2002
specific locus testother cell types/human2.6 mg/L/16H (-enzymatic activation step)MUREAV 724,76,2011
specific locus testother cell types/human1.1 mg/L/16H (+enzymatic activation step)MUREAV 724,76,2011
specific locus testother cell types/human50 mg/L/3H (+enzymatic activation step)MUTAEX 24,35,2009
specific locus testother cell types/human10 µmol/L/24HEMMUEG 54,737,2013
sperm morphologyintraperitoneal/mouse625 mg/kg/5D-continuousMUREAV 124,235,1983
Unscheduled DNA Synthesisliver/mouse10 µmol/LENMUDM 5,1,1983
Unscheduled DNA Synthesisother cell types/rat3200 µmol/LSHKKAN 22,300,1980
Unscheduled DNA Synthesissperm/rat100 µmol/LENMUDM 6,273,1984
Unscheduled DNA Synthesisintraperitoneal/rat10 mg/kgCALEDQ 22,211,1984
Unscheduled DNA Synthesis/Salmonella typhimurium1 µgECEBDI 50,271,1982
Unscheduled DNA Synthesisfibroblast/human100 µmol/L/5HIJCNAW 16,284,1975
Unscheduled DNA SynthesisHeLa cell/human10 µmol/LCNREA8 38,2621,1978
Unscheduled DNA Synthesisliver/human1 µmol/LBANRDU 13,101,1982
Unscheduled DNA Synthesisliver/rat2 µg/LMUREAV 190,159,1987
Unscheduled DNA Synthesisoral/rat5 mg/kgMUREAV 150,383,1985
Unscheduled DNA Synthesisliver/human0.1 µmol/L/44HMUREAV 559,211,2004
Unscheduled DNA Synthesisliver/human0.00002 µmol/L/24HFCTOD7 39,999,2001
Unscheduled DNA Synthesisliver/rat0.1 mg/L/18HMUREAV 472,75,2000
Unscheduled DNA Synthesisother cell types/rabbit10 µmol/LCRNGDP 8,401,1987
Unscheduled DNA Synthesisliver/hamster10 nmol/LENMUDM 6,1,1984

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
intraperitoneal/mouse 100 mg/kg (10D pregnant)Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)

Reproductive: Specific developmental abnormalities: Musculoskeletal system
KAIZAN 37,239,1962
intraperitoneal/mouse 100 mg/kg (8D pregnant)Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants)KAIZAN 37,239,1962
intraperitoneal/mouse 625 mg/kg (5D male)Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)MUREAV 124,235,1983
intraperitoneal/mouse 2500 mg/kg (5D male)Reproductive: Paternal effects: Testes, epididymis, sperm ductMUREAV 124,235,1983
oral/rat 300 mg/kg (9D pregnant)Reproductive: Specific developmental abnormalities: Central nervous systemIARCCD 4,112,1973

Tumorigenic Data and References

Route/OrganismDoseEffectReference
implant/mouse lowest published toxic dose: 96 mg/kgTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Kidney, Ureter, and Bladder: Bladder tumors
GMCRDC 17,383,1975
implant/rat lowest published toxic dose: 22 mg/kgTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Skin and Appendages: Tumors

Tumorigenic: Tumors at site of application
CNREA8 33,2489,1973
intraperitoneal/mouse lowest published toxic dose: 60 mg/kg/2W- intermittentTumorigenic: Neoplastic by RTECS criteria

Lung, Thorax, or Respiration: Tumors
TXAPA9 82,19,1986
intraperitoneal/rabbit lowest published toxic dose: 3600 mg/kg/40W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Kidney, Ureter, and Bladder: Bladder tumors
CNREA8 27,838,1967
intraperitoneal/rat lowest published toxic dose: 192 mg/kg/4W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Lung, Thorax, or Respiration: Tumors

Skin and Appendages: Tumors
CNREA8 32,1554,1972
intratracheal/hamster lowest published toxic dose: 8800 mg/kg/73W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Kidney, Ureter, and Bladder: Bladder tumors
JNCIAM 50,503,1973
multiple/hamster lowest published toxic dose: 3370 mg/kg/37W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Kidney, Ureter, and Bladder: Bladder tumors
INURAQ 14,206,1976
oral/cat lowest published toxic dose: 11 gm/kg/69W- continuousTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Lung, Thorax, or Respiration: Tumors
CNREA8 11,280,1951
oral/chicken lowest published toxic dose: 2800 mg/kg/13W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors

Liver: Tumors
BJCAAI 9,163,1955
oral/Dog lowest published toxic dose: 2625 mg/kg/25W- continuousTumorigenic: Neoplastic by RTECS criteria

Liver: Tumors
CNREA8 10,266,1950
oral/hamster lowest published toxic dose: 9240 mg/kg/44W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
GANNA2 59,239,1968
oral/mouse toxic dose: 13 gm/kg/53W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Kidney, Ureter, and Bladder: Bladder tumors
JCPCBR 19,591,1979
oral/mouse toxic dose: 13140 mg/kg/1Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Skin and Appendages: Tumors
TXAPA9 41,535,1977
oral/mouse lowest published toxic dose: 792.8 mg/kg/12W- continuousTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Colon tumors

Tumorigenic: Increased incidence of tumors in susceptible strains
MUREAV 477,125,2001
oral/mouse lowest published toxic dose: 2738 mg/kg/2.5Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Kidney, Ureter, and Bladder: Bladder tumors
JTEHD6 33,327,1991
oral/mouse lowest published toxic dose: 7840 mg/kg/49W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Kidney, Ureter, and Bladder: Bladder tumors
EJCAAH 5,41,1969
oral/rabbit lowest published toxic dose: 6500 mg/kg/65W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Kidney, Ureter, and Bladder: Bladder tumors

Kidney, Ureter, and Bladder: Kidney tumors
PAMIAD 32,177,1968
oral/rabbit toxic dose: 14 gm/kg/56W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Kidney, Ureter, and Bladder: Bladder tumors

Reproductive: Tumorigenic effects: Ovarian tumors
CNREA8 27,838,1967
oral/rat lowest published toxic dose: 4368 mg/kg/2Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Kidney, Ureter, and Bladder: Bladder tumors
JJCREP 84,237,1993
oral/rat lowest published toxic dose: 672 mg/kg/8W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
TXAPA9 74,63,1984
oral/rat lowest published toxic dose: 1.008 mg/kg/6W- continuousTumorigenic: Neoplastic by RTECS criteria

Blood: Tumors

Tumorigenic: Facilitates action of known carcinogens
CALEDQ 154,121,2000
oral/rat lowest published toxic dose: 60 mg/kg/3D- intermittentTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Tumorigenic: Facilitates action of known carcinogens
TXCYAC 171,161,2002
oral/rat lowest published toxic dose: 60 mg/kgTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Tumorigenic: Facilitates action of known carcinogens
TXCYAC 200,69,2004
oral/rat lowest published toxic dose: 1750 mg/kg/25W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Tumorigenic: Facilitates action of known carcinogens
TXAPA9 194,132,2004
oral/rat lowest published toxic dose: 105 mg/kg/2W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Tumorigenic: Facilitates action of known carcinogens
ARTODN 78,460,2004
oral/rat lowest published toxic dose: 420 mg/kg/6WTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Liver: Tumors

Tumorigenic: Facilitates action of known carcinogens
FCTOD7 49,434,2011
oral/rat toxic dose: 1344 mg/kg/16W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Liver: Hepatitis, fibrous (cirrhosis, post- necrotic scarring)
TXAPA9 19,687,1971
oral/rat toxic dose: 2940 mg/kg/35W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
TXAPA9 19,687,1971
oral/rat toxic dose: 2100 mg/kg/25W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
CALEDQ 19,55,1983
oral/rat toxic dose: 1220 mg/kg/19W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Skin and Appendages: Tumors
CNREA8 22,1002,1962
oral/rat toxic dose: 2100 mg/kg/30W- continuousTumorigenic: Carcinogenic by RTECS criteria

Skin and Appendages: Tumors
TXAPA9 5,526,1963
oral/rat toxic dose: 2610 mg/kg/23W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Skin and Appendages: Tumors
JNCIAM 10,1201,1950
parenteral/chicken lowest published toxic dose: 3430 mg/kg/3YTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Lung, Thorax, or Respiration: Tumors

Kidney, Ureter, and Bladder: Kidney tumors
BJCAAI 8,147,1954
parenteral/rat lowest published toxic dose: 1700 mg/kg/17W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Gastrointestinal: Tumors

Skin and Appendages: Tumors
CNREA8 6,617,1946
skin/rat toxic dose: 2590 mg/kg/23W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Ear: Tumors

Liver: Tumors
CNREA8 28,234,1968
skin/rat lowest published toxic dose: 260 mg/kg/71W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Endocrine: Adrenal cortex tumors

Skin and Appendages: Tumors
JNCIAM 10,1201,1950
subcutaneous/mouse lowest published toxic dose: 400 mg/kg (15D pregnant)Tumorigenic: Equivocal tumorigenic agent by RTECS criteria

Reproductive: Tumorigenic effects: Transplacental tumorigenesis

Liver: Tumors
IJCNAW 20,293,1977
subcutaneous/rat lowest published toxic dose: 2600 mg/kg/43W- intermittentTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
JJCREP 85,794,1994

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
implant/rat lowest published toxic dose: 280 mg/kgLiver: Other changes

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Metabolism (intermediary): Lipids including transport
TXCYAC 196,155,2004
In Vitro/Human, colon tumor Inhibitor Concentration (50 percent kill): >100 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 28,46,2014
intraperitoneal/mouse lowest published lethal dose: 2200 mg/kgMUREAV 747,164,2012
intraperitoneal/mouse lethal dose (50 percent kill): 470 mg/kgENTOX* -,512,2005
intraperitoneal/rat Lethal dose: >200 mg/kgARTODN 56,151,1985
oral/mouse lethal dose (50 percent kill): 810 mg/kgENTOX* -,512,2005
oral/mouse lethal dose (50 percent kill): 810 mg/kgPeripheral Nerve and Sensation: Spastic paralysis with/without sensory change

Behavioral: Altered sleep time (including change in righting reflex)

Kidney, Ureter, and Bladder: Other changes in urine composition
PSEBAA 143,1117,1973
oral/rat lowest published toxic dose: 150 mg/kgLiver: Changes in liver weight

Endocrine: Changes in thymus weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
CTSRCS 288,371,1997
oral/rat lowest published toxic dose: 345 mg/kgSkin and Appendages: Other: Breast

Tumorigenic: Tumor types after systemic administration not seen spontaneously
NATUAS 189,204,1961
oral/rat lowest published toxic dose: 517.5 mg/kgBehavioral: General anestheticNATUAS 189,204,1961
oral/rat lowest published toxic dose: 1000 mg/kgLiver: Other changesTXCYAC 324,43,2014
Tank with water/Zebrafish lethal concentration (50 percent kill): 6.9 µmol/L 3DTXCYAC 281,25,2011
Tank with water/Zebrafish Lowest published lethal concentration: 5 µmol/L 3DAquatic organism effects: Growth retardation

Reproductive: Other developmental abnormalities
TXCYAC 281,25,2011
unreported route/rat lowest published toxic dose: 1000 mg/kgLiver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Hepatic microsomal mixed oxidase (dealkylation, hydroxylation, etc.)
TOLED5 188,91,2009
unreported route/rat lowest published toxic dose: 100 mg/kgLiver: TumorsTOLED5 188,91,2009

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
oral/rat lowest published toxic dose: 564 mg/kg/3W- continuousLiver: Fatty liver degeneration

Liver: Hepatitis, fibrous (cirrhosis, post- necrotic scarring)

Liver: Other changes
APJAAG 36,1039,1986
oral/rat lowest published toxic dose: 336 mg/kg/16W- continuousNutritional and Gross Metabolic: Weight loss or decreased weight gainTOSCF2 55,44,2000
oral/rat lowest published toxic dose: 42 mg/kg/2W- continuousLiver: Other changesTOSCF2 55,44,2000
oral/rat lowest published toxic dose: 672 mg/kg/16W- continuousTumorigenic: Cells (cultured) transformedTOSCF2 55,44,2000
oral/rat lowest published toxic dose: 1344 mg/kg/4W- continuousLiver: Hepatitis (hepatocellular necrosis), zonalTOSCF2 55,44,2000
oral/rat lowest published toxic dose: 40 mg/kg/5D- intermittentLiver: Other changesTOXID9 72,360,2003
oral/rat lowest published toxic dose: 232.96 mg/kg/104W- intermittentLiver: TumorsMUTAEX 26,605,2011
oral/rat lowest published toxic dose: 840 mg/kg/28D- intermittentRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldMUREAV 780-781,2,2015
oral/rat lowest published toxic dose: 840 mg/kg/14D- intermittentLiver: TumorsMUREAV 780-781,2,2015
oral/rat lowest published toxic dose: 1680 mg/kg/14D- intermittentLiver: Changes in liver weightMUREAV 780-781,2,2015
oral/rat lowest published toxic dose: 1680 mg/kg/28D- intermittentLiver: Changes in liver weightMUREAV 780-781,2,2015
oral/rat : 1200 mg/kg/4D- intermittentLiver: Other changesTXCYAC 324,43,2014
oral/rat lowest published toxic dose: 94.1 mg/kg/16W- intermittentLiver: Other changesTOXID9 78,224,2004
oral/rat lowest published toxic dose: 504 mg/kg/6W- continuousLiver: Other changes

Nutritional and Gross Metabolic: Changes in: Other metals

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other enzymes
TOXID9 12,402,2008
unreported route/rat lowest published toxic dose: 84 mg/kg/28D- intermittentLiver: Other changesTOXID9 -,132,2009
unreported route/rat lowest published toxic dose: 7.88 gm/kg/12W- intermittentLiver: Changes in liver weightTOXID9 66,181,2002

Reviews

OrganizationStandardReference
TOXICOLOGY REVIEW32XPAD -,49,1975
TOXICOLOGY REVIEW85CVA2 5,63,1970
TOXICOLOGY REVIEWZEKBAI 71,32,1968
TOXICOLOGY REVIEWADCSAJ 13,271,1970
TOXICOLOGY REVIEWBLFSBY 24,129,1983
TOXICOLOGY REVIEWARPTDI 43,485,2003
TOXICOLOGY REVIEWMUREAV 567,347,2004
TOXICOLOGY REVIEWMUTAEX 15,431,2000
TOXICOLOGY REVIEWMUREAV 588,88,2005
TOXICOLOGY REVIEWMUREAV 614,77,2007
TOXICOLOGY REVIEWMUREAV 628,31,2007
TOXICOLOGY REVIEWMUREAV 627,10,2007
TOXICOLOGY REVIEWMUREAV 627,59,2007
TOXICOLOGY REVIEWMUREAV 584,1,2005
TOXICOLOGY REVIEWENTOX* -,512,2005
TOXICOLOGY REVIEWHETOEA 25,413,2006
TOXICOLOGY REVIEWTOPADD 32(Suppl. 2),85,2004
TOXICOLOGY REVIEWTOPADD 33,63,2005
TOXICOLOGY REVIEWMUREAV 540,165,2003
TOXICOLOGY REVIEWMUREAV 658,271,2008
TOXICOLOGY REVIEWJTPAE7 18,69,2005
TOXICOLOGY REVIEWJTPAE7 19,111,2006
TOXICOLOGY REVIEWJTPAE7 19,1,2006
TOXICOLOGY REVIEWMUREAV 590,1,2005
TOXICOLOGY REVIEWMUTAEX 23,271,2008
TOXICOLOGY REVIEWMUREAV 705,184,2010
TOXICOLOGY REVIEWMUTAEX 26,223,2011
TOXICOLOGY REVIEWMUREAV 685,21,2010
TOXICOLOGY REVIEWMUREAV 727,16,2011
TOXICOLOGY REVIEWMUREAV 678,152,2009
TOXICOLOGY REVIEWMUREAV 727,104,2011
TOXICOLOGY REVIEWMUREAV 653,99,2008
TOXICOLOGY REVIEWMUREAV 723,108,2011
TOXICOLOGY REVIEWMUREAV 752,45,2013
TOXICOLOGY REVIEWEMMUEG 53,645,2012
TOXICOLOGY REVIEWEMMUEG 53,699,2012
TOXICOLOGY REVIEWTXAPA9 254,86,2011
TOXICOLOGY REVIEWMUREAV 764-765,46,2014
TOXICOLOGY REVIEWMUREAV 769,34,2014
TOXICOLOGY REVIEWMUREAV 783,19,2015
TOXICOLOGY REVIEWMUREAV 783,66,2015
TOXICOLOGY REVIEWMUREAV 783,23,2015
TOXICOLOGY REVIEWMUREAV 786-788,14,2015
TOXICOLOGY REVIEWMUREAV 765,56,2015
TOXICOLOGY REVIEWMUREAV 768,53,2016
TOXICOLOGY REVIEWMUREAV 795,7,2016
TOXICOLOGY REVIEWMUTAEX 31,117,2016
TOXICOLOGY REVIEWMUREAV 802,1,2016

Standards and Regulations

OrganizationStandardReference
Mine Safety and Health Administration (MSHA) STANDARDair-SUSPECTED CARCINOGENDTLWS* 3,8,1973
Occupational Exposure Limit-SWEDENGroup A Carcinogen, JUN2005
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction)see Code of Federal Regulations (CFR) 29,1926.1114CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry)see Code of Federal Regulations (CFR) 29,1910.1014CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards)see Code of Federal Regulations (CFR) 29,1915.1014CFRGBR 29,1915.1000,1993
Occupational Safety and Health Administration (OSHA)-cancer suspect agentCFRGBR 29,1910.1014,1987

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO 2-ACETYLAMINOFLUORENE-airCarcinogen use 29 Code of Federal Regulations (CFR) 1910.1014NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983Hazard Code X6612; Number of Industries 1; Total Number of Facilities 75; Number of Occupations 1; Total Number of Employees Exposed 373

Status in Federal Agencies

OrganizationReference
EPA GENETOX PROGRAM 1988, Inconclusive: Cell transform.-RLV F344 rat embryo
EPA GENETOX PROGRAM 1988, Inconclusive: In vitro UDS-human fibroblast
EPA GENETOX PROGRAM 1988, Inconclusive: In vivo SCE-nonhuman; D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Negative: Cell transform.-SA7/SHE; E coli polA without S9
EPA GENETOX PROGRAM 1988, Negative: In vitro SCE-human lymphocytes; In vitro SCE-human
EPA GENETOX PROGRAM 1988, Negative: S cerevisiae gene conversion; S cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Negative: Sperm morphology-mouse; V79 cell culture-gene mutation
EPA GENETOX PROGRAM 1988, Positive/dose response: Cell transform.-BALB/c-3T3; SHE-clonal assay
EPA GENETOX PROGRAM 1988, Positive: Body fluid assay; Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Positive: CHO gene mutation
EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test; In vitro SCE-nonhuman
EPA GENETOX PROGRAM 1988, Positive: Host-mediated assay; L5178Y cells in vitro-TK test
EPA GENETOX PROGRAM 1988, Positive: In vitro UDS in rat liver
EPA GENETOX PROGRAM 1988, Positive: Mammalian micronucleus; E coli polA with S9
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NTP 10th Report on Carcinogens,2002:Reasonably anticipated to be a human carcinogen
TOP