Isolate Bank – C. difficile Infection Tracking
The C. difficile isolate bank is a collection of isolates obtained through the Emerging Infections Program’s C. difficile infection (CDI) surveillance. This population- and laboratory-based surveillance system operates in ten states, drawing from a population of approximately 12 million people. Isolates represent a convenience sample of both community- and healthcare- associated strains obtained from cases of CDI in the population under surveillance.
Stool aliquots or stool swabs are sent to partner reference laboratories for culture and then sent to CDC for molecular typing and characterization by whole genome sequencing, including multilocus sequence typing (MLST), detection of tcdA, tcdB, cdtA and cdtB toxin genes, and assessment of deletions in the tcdC gene. From 2012-2018, strain typing was performed using capillary-based PCR-ribotyping and results were analyzed against a library of standard profiles using BioNumerics.
On this website you will find further information about the available isolates, as well as the procedure for requesting them.
C. difficile isolates from a subset of cases identified through the Emerging Infections Program (EIP) have been collected since 2009.
A panel of 30 C. difficile EIP isolates that represent the top ten most prevalent PCR-ribotypes collected in 2016 is available through the CDC & FDA Antibiotic Resistance Isolate Bank (AR Isolate Bank). These isolates are highly characterized with whole genome sequencing and antimicrobial susceptibility data availableexternal icon.
A nationally representative sample of C. difficile isolates (based on geographic distribution across the EIP sites) collected during 2010-2011 were submitted to BEI Resources, a research material repository established by the National Institutes of Health / National Institute of Allergy and Infectious Disease and currently managed by the American Type Culture Collection. Accompanying metadata include basic demographic information, year of specimen collection, geographical region, hospitalization, outcome, epidemiologic classification, and molecular data.
- Requests for EIP C. difficile isolates that are currently available through the CDC & FDA Antibiotic Resistance Isolate Bank should be made directly to the AR Isolate Bank (AR Isolate Bank).
- Requests for EIP C. difficile isolates that are currently available through BEI Resources should be made directly to ATCC (BEI Resourcesexternal icon).
- Requests for other EIP C. difficile isolates should come directly to CDC by interested institutions according to the following procedure:
Initiating a Request:
- Requestor will be required to prepare a short request (3-5 sentences) which states the number of isolates needed and associated specific epidemiologic data requested. The request should be sent to Amy Gargis by email, email@example.com.
- CDI surveillance staff will notify requestor if the isolates are available.
- If isolates are available, requestor will submit a formal request using official letterhead with a brief summary of the proposed project, no longer than 2 pages, which includes the following information:
- Objective of the project
- Public health importance
- Scientific methods
- Authorship plans
- Biohazard issues
- Projected timelines
- Requestor’s current association/affiliation and complete contact information
- Number of isolates needed and associated specific epidemiologic data
- Shipping account number
In order to avoid duplication, the scientific work proposed must not already be part of “core” CDI surveillance project activities or contained within a proposal for which permission was already granted to another investigator. CDC will ensure this through the following process:
- All requests will be shared with the appropriate CDC and EIP site staff for approval by email.
- CDI surveillance staff will notify the requestor if the request has been approved or denied.
- Any modifications to the initially proposed use of isolates by receiving institutions should be submitted to the CDC for approval from the appropriate CDC and EIP site staff.
User Fee Agreement:
- If the request is approved, for-profit institutions will be charged $150 per isolate requested and non-profit institutions will be charged $50 per isolate requested if over 10 isolates are requested. Isolates will be free of charge for non-profit institutions requesting ≤ 10 isolates. CDC will send a User Fee agreement for completion. The document requires a limit on dissemination of isolates and includes an agreement to acknowledge the source of these isolates and to limit isolate use to what was proposed in the original request.
- Acknowledgment for EIP CDI surveillance will be in the following standardized format: Centers for Disease Control and Prevention’s Emerging Infections Program Clostridioides difficile infection (CDI) surveillance.
- Requestor will be required to share any abstracts or manuscripts containing EIP isolate data with CDC prior to submission to scientific conferences or peer-reviewed journals to ensure that the source of isolates has been appropriately acknowledged and that the presentation/publication reflects what was proposed by the requestor.
Note: At least one scientist from the CDC should review the abstract or manuscript, even when lead authors are not CDC or EIP site staff.