Biological effects of crude oil vapor. IV. Cardiovascular effects-Dataset

Data Collection Methods

Animals.  Male Sprague-Dawley were housed in pairs under controlled light cycle (12 h light/12 h dark) and temperature (22 – 25 °C) conditions and were provided tap water and food ad libitum.

Exposure system.  The exposure system used to generate COV is described in McKinney et al. (2021).  In Experiment 1, rats were exposed to a single (acute), 6-h exposure to COV (300 ppm) or filtered air in a whole-body inhalation system and euthanized 1 or 28 d following the exposure.  In Experiment 2, rats were exposed to filtered air or 300 ppm COV 6 h/d × 4 d/wk × 4 wk (sub chronic) and euthanized 1, 28 or 90 d following the exposure. After each 6 h exposure, the animals were returned to the colony room.  In experiment 1, animals were euthanized 1 or 28 d after exposure and in Experiment 2 they were euthanized 1, 28 or 90 d after exposure.

Measures.  A detailed description of the methods can be found in the link below.  To measure the effects of the exposures on cardiovascular function, vascular and cardiac responses to adrenergic agonists were analyzed.  To determine mechanisms that may underlie change in cardiovascular function, reactive oxygen species were measured using ELISAs for nitrate/nitrite or hydrogen peroxide, or by measuring immunofluorescence in heart and kidney tissues.  Quantitative RT-PCR was used to measure transcripts associated with vascular and renal function.  Protein arrays were also used to determine if proteins associated with vascular or renal disease were altered in response to the exposure.