08/23/2022: Lab Advisory: Monkeypox Virus Testing Considerations to Prevent False Positive Test Results
Audience: Clinical Laboratory Professionals
Level: Laboratory Advisory
To prevent false positive test results, CDC recommends that laboratory professionals perform repeat testing to verify positive diagnostic results for Orthopoxvirus or Monkeypox virus DNA in specimens with high Cycle Threshold (Ct) values from persons who do not meet identified epidemiologic risk criteria for monkeypox.
Review laboratory test results (including raw data, PCR curves, etc.) carefully before you report results. Because molecular tests (e.g., real-time PCR tests) are very sensitive, cross-contamination is possible. If you obtain a high Ct value (generally ~34 or higher), CDC recommends to immediately re-extract and re-test to ensure there was no cross-contamination. CDC suggests this approach based on high Ct value alone, even in the absence of epidemiologic information. When possible, re-extract and re-test before you report results. Obtain approval from your CLIA director before you implement this recommendation in your laboratory.
Refer to the Health Alert Network (HAN) Health Update for more information on testing for monkeypox.
- Monkeypox Information for Laboratory Personnel
- How to Report Results from Orthopoxvirus, Non-Variola Orthopoxvirus, and Monkeypox Virus Laboratory Diagnostic Testing
- Laboratory Procedures and Biosafety Guidelines
- U.S. Monkeypox 2022: Situation Summary
- CDC Laboratory Outreach Communication System (LOCS)
- LOCS Calls
- If you are a clinical or public health laboratory professional, contact LOCS.
- For media inquiries, contact the CDC Newsroom.
- For all other questions, contact CDC-INFO.