Evaluating Mosquitoes for Insecticide Resistance
Centers for Disease Control and Prevention
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 About this course
Course Topics

 Disease Vectors

 Strategies for Resistance Management

 Resistance Assays
 Bottle Bioassay
 Larval Bioassay
 Microplate Assay
 Interpretation of Results

 Case Studies
Supplements

 Links and Resources

 Companion Materials

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Bottle Bioassays

The bottle bioassay allows resistance levels to be established for populations of mosquitoes reared in an insectary or collected in the field. The major advantages of the bottle assay are that any concentration of any insecticide may be evaluated at the one time. Secondly, the technique is simple and rapid.

The goal of the bioassay is to measure the time it takes for a given insecticide to kill the adult mosquito.

Materials Needed (where to get materials)

  • 250 ml clean empty bottles with lids (one bottle for each insecticide sample and one bottle to function as control).*
  • acetone (reagent grade)
  • technical (analytical grade) or formulation grade insecticide
  • pippette (one for each insecticide sample)
  • pippette (one to be used for dosing each bottle with acetone)
  • aspirator (learn to make your own)
  • timer (digital counter capable of counting seconds)
  • adult mosquitoes (10-20 for each bottle)

    *Alternative size bottles can be used as long as they are all the same size for each test.

Overview

This procedure has six steps. Steps are repeated for each insecticide formulation used.

  Beaker calibration (same for both bottle and larval assays)
This should be known before beginning.
Step 1:

Preparing the formulation.

Step 2:

Coating the bottle.

Step 3:

Loading the mosquitoes in the bottles.

Step 4:

Timing and plotting mosquito mortality.

Step 5: Scoring the data.
Step 6: Clean up (same for both bottle and larval assays).
  Data Interpretation (same for both bottle and larval assays)
Learn how to plot and analyze the outcomes of the bioassays in order to create reports of experiments.
  Practice questions

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This page last reviewed May 7, 2002

Centers for Disease Control and Prevention
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