Skip directly to search Skip directly to A to Z list Skip directly to navigation Skip directly to site content Skip directly to page options
CDC Home

CDC Bottle Bioassay

The CDC bottle bioassay determines if a particular formulation (combination of the active ingredient in the insecticide and inactive ingredients) is able to kill an insect vector, such as a mosquito, at a specific location at a given time. It can detect resistance to insecticides in mosquitoes and other insects. The technique is simple, rapid, and economical, compared with alternatives. The results can help guide the choice of insecticide used for spraying.

If your institution or program would like to order a bottle bioassay kit, which contains bottles, insecticide formulations, manual, and instructional video, please contact CDC to discuss the collaboration at To order only the bottle bioassay insecticide resistance formulation, please send your request to The formulations are free.

A practical laboratory manual that describes how to perform and interpret the CDC bottle bioassay is now available online in three languages:

  •   Insert 1 - Revised Box 5: Interpretation of Data for Resistance Management Purposes
  •   Insert 2 - Enhanced Surveillance Protocol for the CDC Intensity Bottle Bioassay

Below is a companion instructional video.

Download this video [mp4]

Contact Us:
  • Centers for Disease Control and Prevention
    1600 Clifton Rd.
    Atlanta, GA 30333
  • 1-800-CDC-INFO
    TTY: 1-888-232-6348
    Hours of Operation
    8am-8pm EST/ Monday-Friday
    Closed Holidays
  • Contact CDC-INFO
  • Page last reviewed: February 3, 2015
  • Page last updated: February 3, 2015 The U.S. Government's Official Web PortalDepartment of Health and Human Services
Centers for Disease Control and Prevention   1600 Clifton Rd. Atlanta, GA 30329-4027, USA
800-CDC-INFO (800-232-4636) TTY: (888) 232-6348 - Contact CDC-INFO