Illustration of Parotid Gland and Instructions for Collection of Buccal Fluid
Adapted from the Illinois Dept. of Public Health – Div. of Laboratories (Chicago Virology Section)
Preferred specimen: Parotid gland duct swab for viral culture collected within 9 days of onset of symptoms.
Collection of a mumps specimen for viral and PCR testing: Massage the parotid (salivary) glands for 30 seconds.
Specimen Preparation: Swabs should be placed in 2 ml of standard viral transport medium. Allow the swab to remain in VTM for at least 1 hour (4°C). Ream the swab around the rim of the tube to retain cells and fluid in the tube. The swab can be broken off and left in the tube or discarded.
Storage and Shipment: Following collection, samples should be maintained at 4°C and shipped on cold packs (4°C) within 24 hours. If there is a delay in shipment, the sample is best preserved by freezing at −70°C. Frozen samples should be shipped on dry ice.
Images and logos on this website which are trademarked/copyrighted or used with permission of the trademark/copyright or logo holder are not in the public domain. These images and logos have been licensed for or used with permission in the materials provided on this website. The materials in the form presented on this website may be used without seeking further permission. Any other use of trademarked/copyrighted images or logos requires permission from the trademark/copyright holder...more
This graphic notice means that you are leaving an HHS Web site. For more information, please see the Exit Notification and Disclaimer policy.