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Registry of Toxic Effects of Chemical Substances (RTECS)

Toluene-2,4-diamine

RTECS #

XS9625000

CAS #

95-80-7

Updated

April 2017

Molecular Weight

122.19

Molecular Formula

C7H10N2

Synonyms

1,3-Benzenediamine, 4-methyl-
1,3-Diamino-4-methylbenzene
2,4-Diamino-1-methylbenzene
2,4-Diamino-1-toluene
2,4-Diaminotoluen (Czech)
2,4-Diaminotoluene
2,4-Diaminotoluol
2,4-Tolamine
2,4-Tolylenediamine
3-Amino-p-toluidine
4-Methyl-1,3-benzenediamine
4-Methyl-m-phenylenediamine
5-Amino-o-toluidine
Benzofur MT
Brown for Fur T
C.I. 76035
C.I. Oxidation Base 20
C.I. Oxidation Base 200
C.I. Oxidation Base 35
Developer 14
Developer B
Developer DB
Developer DBJ
Developer MC
Developer MT
Developer MT-CF
Developer MTD
Developer T
Eucanine GB
Fouramine
Fouramine J
Fourrine 94
Fourrine M
MTD
m-Toluenediamine
m-Toluylendiamin (Czech)
m-Toluylenediamine
m-Tolyenediamine
m-Tolylenediamine
Nako TMT
NCI-C02302
Pelagol Grey J
Pelagol J
Pontamine developer TN
RCRA waste number U221
Renal MD
TDA
Tertral G
Tolylene-2,4-diamine
Zoba GKE

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
eye /rabbit 100 mg/24H moderate85JCAE -,477,1986
eye /rabbit 100 mg/24HTAEHC* IP:74,1,1987
skin /rabbit 500 mg/24HTAEHC* IP:75,1,1987
skin /rabbit 500 mg/24H mild85JCAE -,477,1986

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
body fluid assaySalmonella typhimurium/rat70 mg/kgJJIND8 76,291,1986
Cytogenetic Analysisovary/hamster4 mmol/LMUREAV 265,45,1992
DNA adductoral/rat84 mg/kg/3W-continuousMUREAV 367,209,1996
DNA Damageintraperitoneal/rat37 mg/kgCRNGDP 6,1285,1985
DNA Damageintraperitoneal/mouse240 mg/kgMUREAV 391,201,1997
DNA Damageoral/mouse60 mg/kgMUREAV 440,1,1999
DNA Damagefibroblast/human100 µmol/LMUREAV 127,107,1984
DNA Damagemammary gland/human98 mg/L/24H (-enzymatic activation step)MUREAV 588,47,2005
DNA Damageother cell types/human1.46 mmol/L/20HTXCYAC 213,138,2005
DNA Damageliver/human1.46 mmol/L/20HTXCYAC 213,138,2005
DNA Damageother cell types/human0.316 mmol/l/48HMUREAV 696,21,2010
DNA Damageother cell types/human31.6 mmol/l/48HMUREAV 696,21,2010
DNA Damageliver/human0.316 mmol/l/48HMUREAV 696,21,2010
DNA Damageother cell types/mouse0.125 mmol/LMUREAV 715,25,2011
DNA Damageoral/rat1000 mg/kg/21H- intermittentMUREAV 786-788,3,2015
DNA Damageoral/rat300 mg/kg/3D- intermittentMUREAV 786-788,151,2015
DNA Damageother cell types/human1 µmol/L/24HMUTAEX 31,83,2016
DNA Damageother cell types/human1 mmol/L/24H (+enzymatic activation step)MUREAV 760,23,2014
DNA Damageother cell types/mouse625 µmol/L/24HMUREAV 810,13,2016
DNA inhibitionintraperitoneal/mouse111 mg/kgMUREAV 91,75,1981
micronucleus testliver/human2.14 mmol/L/20HTXCYAC 213,138,2005
micronucleus testother cell types/human2.14 mmol/L/20HTXCYAC 213,138,2005
micronucleus testoral/rat700 mg/kg/14D- intermittentMUREAV 747,234,2012
micronucleus testoral/rat700 mg/kg/28D- intermittentMUREAV 747,234,2012
micronucleus testother cell types/human134 mg/L/3H (+enzymatic activation step)MUREAV 767,28,2014
micronucleus testlymphocyte/human1222 mg/L/3H (+enzymatic activation step)MUREAV 767,28,2014
micronucleus testother cell types/human316 µmol/L/3HMUREAV 724,7,2011
micronucleus testoral/rat125 mg/kgMUTAEX 24,9,2009
micronucleus testoral/rat125 mg/kg/2D- intermittentMUREAV 698,24,2010
morphological transformembryo/mouse20 mg/L/72HMUREAV 702,100,2010
morphological transformembryo/hamster50 mg/L/24HMUREAV 577S,1,2005
morphological transformfibroblast/mouse10 mg/L/21D (-enzymatic activation step)EMMUEG 35,300,2000
morphological transformembryo/hamster10 mg/L/7DMUREAV 654,108,2008
morphological transformembryo/hamster500 µg/LCALEDQ 3,45,1977
mutation in mammalian somatic cellslymphocyte/mouse198 mg/LMUREAV 135,115,1984
mutation in mammalian somatic cellsovary/hamster2 gm/LMUREAV 135,115,1984
mutation in microorganisms/Salmonella typhimurium625 µg/plate/20M (+enzymatic activation step)MUTAEX 20,217,2005
mutation in microorganisms/Salmonella typhimurium2500 µg/plate/48H (+enzymatic activation step)MUTAEX 20,217,2005
mutation in microorganisms/Salmonella typhimurium10 mg/plate (+enzymatic activation step)ENMUDM 5(Suppl 1),3,1983
mutation in microorganisms/Salmonella typhimurium10 µg/plate (-enzymatic activation step)JJIND8 76,291,1986
mutation in microorganisms/Salmonella typhimurium0.5 mmol/L/4H (+enzymatic activation step)MUREAV 810,13,2016
other mutation test systemsintraperitoneal/mouse200 mg/kgMUREAV 747,164,2012
other mutation test systemsother cell types/human500 µmol/L/24HTIVIEQ 28,46,2014
other mutation test systems/Salmonella typhimurium60 mg/LMUREAV 192,239,1987
other mutation test systemsovary/hamster6 mmol/LMUREAV 265,45,1992
phage inhibition capacity/Escherichia coli100 mmol/LMDMIAZ 31,11,1979
sex chromosome loss and nondisjunctionparenteral/Drosophila melanogaster5 mmol/LMUREAV 56,31,1977
sex chromosome loss and nondisjunctionoral/Drosophila melanogaster15200 mmol/L/3DMUREAV 48,181,1977
sister chromatid exchangeintraperitoneal/mouse9 mg/kgMUREAV 108,225,1983
specific locus testother cell types/human31.25 mg/L (-enzymatic activation step)MUREAV 695,87,2010
specific locus testskin/mouse5600 mg/kg/28D-continuousMUREAV 608,88,2006
specific locus testother cell types/human75 mg/L/16H (-enzymatic activation step)MUREAV 724,76,2011
specific locus testother cell types/human16 mg/L/16H (+enzymatic activation step)MUREAV 724,76,2011
specific locus testother cell types/human31.25 mg/L/48H (-enzymatic activation step)MUTAEX 24,35,2009
specific locus testother cell types/human10 µmol/L/24HEMMUEG 54,737,2013
Unscheduled DNA Synthesisother cell types/human0.01 mmol/L/20HTXCYAC 213,138,2005
Unscheduled DNA Synthesisliver/human100 µmol/LCNREA8 49,1075,1989
Unscheduled DNA Synthesisoral/rat150 mg/kgENMUDM 4,553,1982
Unscheduled DNA Synthesisliver/rat10 µmol/LMUREAV 315,147,1994

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
oral/rat 1260 mg/kg (10W male)Reproductive: Effects on fertility: Mating performance (e.g., # sperm positive females per # females mated; # copulations per # estrus cycles)JTEHD6 16,753,1985
oral/rat 1260 mg/kg (10W male)Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Prostate, seminal vesicle, Cowper's gland, accessory glands
JTEHD6 16,763,1985
oral/rat 210 mg/kg (1W male)Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct
JTEHD6 25,435,1988
oral/mouse 1200 mg/kg (6-13D pregnant)Reproductive: Effects on fertility: Litter size (e.g., # fetuses per litter; measured before birth)

Reproductive: Effects on newborn: Live birth index (Litter size (e.g., # fetuses per litter; measured after birth)
TCMUD8 7,29,1987

Tumorigenic Data and References

Route/OrganismDoseEffectReference
oral/mouse lowest published toxic dose: 8050 mg/kg/101W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Blood: Lymphoma including Hodgkin's disease
NCITR* NCI-TR-162,1979
oral/mouse toxic dose: 17 gm/kg/2Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Blood: Lymphoma including Hodgkin's disease
NCITR* NCI-TR-162,1979
oral/mouse toxic dose: 8400 mg/kg/2Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
GANNA2 70,453,1979
oral/mouse toxic dose: 16800 mg/kg/2Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
GANNA2 70,453,1979
oral/rat lowest published toxic dose: 2100 mg/kg/90W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Skin and Appendages: Tumors
JJIND8 62,1107,1979
oral/rat toxic dose: 5030 mg/kg/84W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Blood: Lymphoma including Hodgkin's disease
NCITR* NCI-TR-162,1979
oral/rat toxic dose: 9000 mg/kg/36W- continuousTumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
CNREA8 29,1137,1969

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
intraperitoneal/mouse lethal dose (50 percent kill): 480 mg/kgJEPTDQ 3(1-2),149,1979
intraperitoneal/rat lethal dose (50 percent kill): 325 mg/kgJEPTDQ 3(1-2),149,1979
oral/mouse lowest published lethal dose: 380 mg/kgMUREAV 747,164,2012
oral/rat lethal dose (50 percent kill): 590 mg/kg/24HTAEHC* -,1,1987
skin/rabbit lethal dose (50 percent kill): 650 mg/kg/24HTAEHC* -,1,1987
subcutaneous/Dog lowest published lethal dose: 200 mg/kgXPHBAO 271,56,1941
subcutaneous/rabbit lowest published lethal dose: 400 mg/kgBrain and Coverings: Other degenerative changes

Behavioral: Convulsions or effect on seizure threshold

Lung, Thorax, or Respiration: Respiratory stimulation
ZEPTAT 17,59,1915
subcutaneous/rat lowest published lethal dose: 350 mg/kgBehavioral: Somnolence (general depressed activity)

Cardiac: Pulse rate decreased with fall in BP

Lung, Thorax, or Respiration: Respiratory stimulation
ZEPTAT 17,59,1915

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
oral/mouse lowest published toxic dose: 700 mg/kg/7D- intermittentNutritional and Gross Metabolic: Weight loss or decreased weight gainNTPTR* IMM-87034
oral/mouse lowest published toxic dose: 1400 mg/kg/14D- intermittentLiver: Hepatitis (hepatocellular necrosis), zonal

Liver: Changes in liver weight

Endocrine: Changes in spleen weight
NTPTR* IMM-87034
oral/mouse lowest published toxic dose: 1400 mg/kg/14D- intermittentKidney, Ureter, and Bladder: Renal function tests depressed

Blood: Changes in other cell count (unspecified)

Blood: Changes in leukocyte (WBC) count
NTPTR* IMM-87034
oral/mouse lowest published toxic dose: 1400 mg/kg/14D- intermittentImmunological Including Allergic: Increase in cellular immune response

Immunological Including Allergic: Decrease in cellular immune response

Immunological Including Allergic: Decreased immune response
NTPTR* IMM-87034
oral/mouse lowest published toxic dose: 1400 mg/kg/14D- intermittentImmunological Including Allergic: Uncharacterized (multiple organ involvement)

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NTPTR* IMM-87034
oral/mouse lowest published toxic dose: 700 mg/kg/14D- intermittentImmunological Including Allergic: Increase in cellular immune responseNTPTR* IMM-87034
oral/rat lowest published toxic dose: 725 mg/kg/29D- intermittentLiver: Other changesMUREAV 702,40,2010
oral/rat lowest published toxic dose: 2900 mg/kg/29D- intermittentBlood: Changes in platelet count

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
MUREAV 702,40,2010
oral/rat lowest published toxic dose: 700 mg/kg/28D- intermittentLiver: Other changesMUREAV 751,12,2013
oral/rat lowest published toxic dose: 36500 mg/kg/2Y- intermittentKidney, Ureter, and Bladder: Other changes

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
TAEHC* -,1,1987
oral/rat lowest published toxic dose: 1050 mg/kg/70D - continuousReproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct

Reproductive: Paternal effects: Prostate, seminal vesicle, Cowper's gland, accessory glands
TAEHC* IP:74,67,1987
oral/rat lowest published toxic dose: 350 mg/kg/5D- intermittentBiochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effectsTAEHC* -,1,1987
oral/rat lowest published toxic dose: 732.48 mg/kg/16W- continuousLiver: Tumors

Liver: Changes in liver weight
TOXID9 78,367,2004
oral/rat lowest published toxic dose: 2197.44 mg/kg/16W- continuousLiver: Multiple effects

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
TOXID9 78,367,2004

Reviews

OrganizationStandardReference
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 16,83,1978
International Agency for Research on Cancer (IARC)Cancer Review:Human No Adequate DataIMEMDT 16,83,1978
International Agency for Research on Cancer (IARC)Cancer Review:Group 2BIMSUDL 7,56,1987
TOXICOLOGY REVIEWMUREAV 543,201,2003
TOXICOLOGY REVIEWMUREAV 567,227,2004
TOXICOLOGY REVIEWEMMUEG 43,36,2004
TOXICOLOGY REVIEWMUREAV 588,88,2005
TOXICOLOGY REVIEWMUREAV 627,10,2007
TOXICOLOGY REVIEWMUREAV 654,114,2008
TOXICOLOGY REVIEWMUREAV 590,1,2005
TOXICOLOGY REVIEWTXAPA9 243,239,2010
TOXICOLOGY REVIEWMUREAV 705,184,2010
TOXICOLOGY REVIEWMUTAEX 26,223,2011
TOXICOLOGY REVIEWMUREAV 653,99,2008
TOXICOLOGY REVIEWMUREAV 723,108,2011
TOXICOLOGY REVIEWMUTAEX 27,257,2012
TOXICOLOGY REVIEWMUREAV 769,34,2014
TOXICOLOGY REVIEWFCTOD7 55,596,2013
TOXICOLOGY REVIEWMUREAV 783,13,2015
TOXICOLOGY REVIEWMUREAV 795,7,2016
TOXICOLOGY REVIEWMUTAEX 31,117,2016
TOXICOLOGY REVIEWMUREAV 802,1,2016

Standards and Regulations

OrganizationStandardReference
Occupational Exposure Limit-AUSTRIATRK-TMW 0.02 ppm (0.1 mg/m3);KZW 0.08 ppm (0.4 mg/m3), skin, sen, 2007
Occupational Exposure Limit-JAPAN2B carc, MAY2012
Occupational Exposure Limit-POLANDMAC(time-weighted average) 0.04 mg/m3, MAC(short term exposure limit) 0.1 mg/m3, JAN1999
Occupational Exposure Limit-RUSSIAshort term exposure limit 2 mg/m3, Skin, JUN2003
Occupational Exposure Limit-SWEDENGroup B Carcinogen, Sen, JUN2005
Occupational Exposure Limit-SWITZERLANDMAK-week 0.02 ppm (0.1 mg/m3), carc 2, skin, sen, JAN2011

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO TOLUENEDIAMINE-airCarcinogen lowest feasible concentrationNIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983Hazard Code X5386; Number of Industries 6; Total Number of Facilities 130; Number of Occupations 15; Total Number of Employees Exposed 8511; Total Number of Female Employees Exposed 396

Status in Federal Agencies

OrganizationReference
EPA GENETOX PROGRAM 1988, Inconclusive: SHE-clonal assay
EPA GENETOX PROGRAM 1988, Negative: N crassa-aneuploidy; Sperm morphology-mouse
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat; Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: D melanogaster Sex-linked lethal
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, PROPOSED RULEFEREAC 47,27009,82
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NCI Carcinogenesis Bioassay (feed);clear evidence:mouse,ratNCITR* NCI-TR-162,1979
NIOSH Analytical Method, 1994: 2,4-Toluenediamine, 5516
NIOSH CURRENT INTELLIGENCE BULLETIN 53, DECEMBER 1989
NTP 11th Report on Carcinogens,2004:Reasonably anticipated to be a human carcinogen
NTP Carcinogenesis studies; test completed (peer review), October 2000
On EPA IRIS database
OSHA ANALYTICAL METHOD #ID-65
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