Autopsy Specimen Collection Recommendations for Pathologic Evaluation of Suspect Pneumonia Infections

Collection of Tissue Specimens

The preferred specimens would be a minimum of 8 formalin-fixed paraffin-embedded (FFPE) tissue blocks or formalin-fixed wet tissues representing the different pulmonary sites listed below. In addition, specimens from other organs showing significant pathology should be submitted.

FFPE cell blocks made from bronchoalveolar lavage (BAL) can also be submitted in select circumstances, but assay sensitivity may be reduced.

The preferred pulmonary sites include:

  • Hilar lung with segmental bronchi, primary bronchi
  • Trachea, upper and lower
  • Peripheral pulmonary parenchyma from both lungs

Specimens should be included from any other organ showing significant gross or microscopic pathology.