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The CDC collections span the field of public health. Print and electronic resources cover such topics as disease prevention, epidemiology, infectious diseases, global health, chronic diseases, environmental health, injury prevention, and occupational safety and health. The main library is developing a small but growing Landmarks in Public Health collection that includes items of significance to the history of public health. The Pittsburgh library houses an extensive historic collection on mining and mining safety.

The library, in collaboration with the CDC Office of the Chief Science Officer, publishes a weekly public health current literature alert. CDC Science Clips includes newly published CDC journal articles as well as other current articles of interest to CDC partners and the public health community.