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Volume 30, Number 3—March 2024
Dispatch

Highly Pathogenic Avian Influenza A(H5N1) Virus Clade 2.3.4.4b in Domestic Ducks, Indonesia, 2022

Hendra WibawaComments to Author , Putut Eko Wibowo, Arif Supriyadi, Lestari Lestari, Jessiaman Silaban, Aziz Ahmad Fuadi, Anna Januar Fiqri, Retno Wulan Handayani, Sri Handayani Irianingsih, Zaza Fahmia, Herdiyanto Mulyawan, Syafrison Idris, and Nuryani Zainuddin
Author affiliations: Disease Investigation Center Wates, Yogyakarta, Indonesia (H. Wibawa, Lestari, J. Silaban, S.H. Irianingsih, Z. Fahmia, H. Mulyawan); Disease Investigation Center Banjarbaru, Banjarbaru, Indonesia (P.E. Wibowo, A. Supriyadi, A.A. Fuadi, A.J. Fiqri, R.W. Handayani); Directorate General of Livestock and Animal Health Services, Jakarta, Indonesia (S. Idris, N. Zainuddin)

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Table 2

Hemagglutinin inhibition assay titers using 2-fold serial dilutions of virus-specific antiserum in study of highly pathogenic avian influenza A(H5N1) virus clade 2.3.4.4b in domestic ducks, Indonesia, 2022*

Antiserum source, clade, GISAID no.† Antigen source
A/duck/Hulu Sungai Utara/A0522064-06/2022 A/duck/Hulu Sungai Utara/A0522064-03-04/2022 A/duck/Hulu Sungai Utara/A0522067-06-07/2022 A/muscovy duck/ Banjarbaru/A0523532-9/2023
A/chicken/West Java/PWT-WIJ/2006, H5N1 clade 2.1.3.2, EPI_ISL_12700530‡
 <4
 <4
 <4
 16
A/chicken/Barru/BBVM 41-13/2013, H5N1 clade 2.1.3.2a, EPI_ISL_17767706
 16
 16
 16
 16
A /duck/Sukoharjo/BBVW-1428-9/2012, H5N1 clade 2.3.2.1c, EPI_ISL_266808§
 16
 32
 32
 32
A/chicken/Tanggamus/031711076-65/2017, H5N1 clade 2.3.2.1c, EPI_ISL_17767763¶
 16
 32
 16
 32
A/duck/Laos/XBY004/2014, H5N6 clade 2.3.4.4b, EPI_ISL_168385
 8
 16
 8
 16
A/duck/Hulu Sungai Utara/A0522064-03-04/2022, H5N1 clade 2.3.4.4b# 512 512 128 128

*Viruses were isolated by using the World Organisation for Animal Health protocol (7). The 3 viruses isolated from Hulu Sungai Utara in April 2022 and the virus isolated from Banjarbaru in July 2023 were used as antigen sources. †GISAID (https://www.gisaid.org). ‡H5N1 clade 2.1.3.2 vaccine-seed strain used in Indonesia since 2009. §H5N1 clade 2.3.2.1c vaccine strain used during 2012–2020. ¶H5N1 clade 2.3.2.1c vaccine strain used since 2021. #Homologous antiserum for H5N1 clade 2.3.4.4b viruses isolated from the initial outbreak in Hulu Sungai Utara, April 2022.

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