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Volume 29, Number 9—September 2023
Research

Interspecies Transmission of Swine Influenza A Viruses and Human Seasonal Vaccine-Mediated Protection Investigated in Ferret Model

Pauline M. van DiemenComments to Author , Alexander M.P. Byrne, Andrew M. Ramsay, Samantha Watson, Alejandro Nunez, Ana v Moreno, Chiara Chiapponi, Emanuela Foni, Ian H. Brown, Sharon M. Brookes, and Helen E. EverettComments to Author 
Author affiliations: Animal and Plant Health Agency, Addlestone, UK (P.M. van Diemen, A.M.P. Byrne, A.M. Ramsay, S. Watson, A. Nunez, I.H. Brown, S.M. Brookes, H.E. Everett); Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy (C. Chiapponi, E. Foni, A. Moreno)

Main Article

Figure 3

Nasal shedding of viral RNA monitored in pigs intranasally inoculated with influenza A virus strains A/swine/England/1353/2009 (H1pdmN1) (A) or A/Pavia/65/2016 (H1avN1) (B) and in naive or vaccinated ferrets cohoused in the same airspace as inoculated pigs. Viral RNA was quantified by real-time quantitative reverse transcription PCR in longitudinal nasal samples collected daily until 14 dpi (pigs) or 14 dpc (ferrets) and is expressed as REU based on an RNA quantification standard prepared from the corresponding virus stock. In vaccinated ferrets (n = 4) exposed to the H1pdmN1 strain, nasal shedding of viral RNA between 6 dpc and 12 dpc was significantly different from the naive ferret group (p<0.05). Results for the remaining ferret in this group are shown as outlier data (hollow green circles). dpc, days postcontact; dpi, days postinoculation; REU, relative equivalent unit; TCID50, 50% tissue culture infectious dose.

Figure 3. Nasal shedding of viral RNA monitored in pigs intranasally inoculated with influenza A virus strains A/swine/England/1353/2009 (H1pdmN1) (A) or A/Pavia/65/2016 (H1avN1) (B) and in naive or vaccinated ferrets cohoused in the same airspace as inoculated pigs. Viral RNA was quantified by real-time quantitative reverse transcription PCR in longitudinal nasal samples collected daily until 14 dpi (pigs) or 14 dpc (ferrets) and is expressed as REU based on an RNA quantification standard prepared from the corresponding virus stock. In vaccinated ferrets (n = 4) exposed to the H1pdmN1 strain, nasal shedding of viral RNA between 6 dpc and 12 dpc was significantly different from the naive ferret group (p<0.05). Results for the remaining ferret in this group are shown as outlier data (hollow green circles). dpc, days postcontact; dpi, days postinoculation; REU, relative equivalent unit; TCID50, 50% tissue culture infectious dose.

Main Article

Page created: July 05, 2023
Page updated: August 20, 2023
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