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Volume 23, Number 12—December 2017
Dispatch

Phylogenetic Characterization of Crimean-Congo Hemorrhagic Fever Virus, Spain

Eva Ramírez de Arellano, Lourdes Hernández, M. José Goyanes, Marta Arsuaga, Ana Fernández Cruz, Anabel Negredo1, and María Paz Sánchez-Seco1Comments to Author 
Author affiliations: National Center of Microbiology Arbovirus and Imported Viral Diseases Laboratory, Madrid, Spain (E. Ramírez de Arellano, L. Hernández, A. Negredo, M. Paz Sánchez-Seco); Gregorio Marañón Hospital, Madrid (M.J. Goyanes, A. Fernández Cruz); La Paz Hospital, IdiPaz, Madrid (M. Arsuaga)

Main Article

Table

RT-PCR primers used for generation of amplicons used for sequencing CCHFV full genome from strains isolated from patient samples in Spain, 2016*

Primer pair
 5′ → 3′ sequence
 5′ → 3′ position relative to reference strain AB1-2009
 Amplified segment, kb
 Temperature, °C†
CRCON+1 RWAAYGGRCTTRTGGAYACYTTCAC 123–147 S, 1.4 50.5
CRCON1R-S
 TCTCAAAGATAYCGTTGCCGCAC
 1,651–1,673
CRCON1F-M TCTCAAAGAAAATACTTGCGGC 1–22 M, 2.3 48
CRCON4R-M GCATYTCAGCATCTATTGCATT 2,326–22,347
CRCONF4F-M TTGTAGAYCAAAGACTRGGCAG 1,775–1,796 M, 2.1 48
CRCON6R-M
 GCCYGCTTCAATCAAGCTACA
 3,829-3,849
CRCON6F-M TCAATTGAGGCACCATGGGG 3,280–3,299 M, 2.1 50
CRCON8R-M
 TCTCAAAGATATAGTGGCGGCA
 5,348–5,369
CRCON1F-L TCTCAAAGATATCAATCCCCCC 1–22 L, 2.2 48
CRCON3R-L
 AGTGTCGAAAATGTRCAAATCTC
 2,202--2224
CRCON3F-L CCTGAAAGTGACCTCACCCGC 1,562–1,582 L, 3.1 55
CRCON6R-L
 TTGGCATGCTTGCAGGGCTTAG
 4,656–4,677
CRCON7F-L TGCTTGCAGGGCTTAGTAGGCT 4,661–4,682 L, 2.3 50.5
CRCON9R-L
 TCATGCATGCAACCACTGAAAT
 6,948-6,969
CCHF-L2F GAAGAGCTATATGACATAAGGC 6,137–6,158 L, 1.6 48
CCHF-L1R
 TTGGCACTATCTTTCATTTGAC
 7,752–7,773
CRCON10F-L GGTAGTTCAGATGATTACGCAAA 7,616–7,638 L, 1.4 48
CRCON10R-L
 CCTGTTAATTGTTTGCCACAA
 9,019–9,039
CRCON7200F ATGCAACAGGTTCTGAAAAATG 7,199–7,220 L, 3.5 48
CRCON11R-L
 ATGCTCCTAGTGATGCCATAATG
 10,743–10,765
CRCON12F-L TCTTTTGAAGGTGAAGCATCTTG 10,585–10,607 L, 1.6 48
CCHF-L2R
 TCTCAAAGAAATCGTTCCCCCCAC
 12,149–12,172
*F, forward; L, large segment; M, medium segment; R, reverse; RT-PCR, reverse transcription PCR; S, small segment.
†Melting temperature used.

Main Article

1These authors contributed equally to this article.

Page created: November 16, 2017
Page updated: November 16, 2017
Page reviewed: November 16, 2017
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