1,3-Butadiene, hexachloro-

RTECS #

EJ0700000

CAS #

87-68-3
See: NMAM or OSHA Methods

Updated

October 2017

Molecular Weight

260.74

Molecular Formula

C₄Cl₆

Synonyms

1,1,2,3,4,4-Hexachloro-1,3-butadiene
1,3-Hexachlorobutadiene
Dolen-pur
GP-40-66:120
HCBD
Hexachlor-1,3-butadien (Czech)
Hexachlorbutadiene
Hexachloro-1,3-butadiene
Hexachlorobutadiene
Hexachlorobutadiene (ACGIH)
Perchlorobutadiene
RCRA waste number U128

Skin and Eye Irritation and References

Route/Organism	Dose	Effect	Reference
eye /rabbit	162 mg	mild	EJTXAZ 9,171,1976
eye /rabbit	500 mg/24H	mild	85JCAE -,116,1986
skin /rabbit	810 mg/24H	moderate	EJTXAZ 9,171,1976
skin /rabbit	500 mg/24H	mild	85JCAE -,116,1986

Mutation Data and Reference

System Test	Route/Organism/Tissue	Dose	Reference
Cytogenetic Analysis	lymphocyte/human	1 mg/L	CYGEDX 22(4),46,1988
Cytogenetic Analysis	oral/mouse	2 mg/kg	CYGEDX 22(4),46,1988
Cytogenetic Analysis	inhalation/mouse	10 mg/m³	CYGEDX 22(4),46,1988
Cytogenetic Analysis	lung/hamster	6.3 mg/L/4H (+/-enzymatic activation step)	MUREAV 699,47,2010
DNA Damage	liver/human	10 µmol/l/48H	MUREAV 696,21,2010
DNA Damage	liver/human	1 mmol/l/48H	MUREAV 696,21,2010
DNA Damage	liver/human	1 µmol/l/48H	MUREAV 696,21,2010
morphological transform	embryo/hamster	10 mg/L	CALEDQ 23,297,1984
mutation in microorganisms	/Salmonella typhimurium	1 mg/plate (+enzymatic activation step)	DHEFDK FDA-78-1046,1978
mutation in microorganisms	/Salmonella typhimurium	320 µg/plate (-enzymatic activation step)	CRNGDP 7,431,1986
sister chromatid exchange	ovary/hamster	1400 µg/L	EMMUEG 10(Suppl 10),1,1987
Unscheduled DNA Synthesis	embryo/hamster	2 mg/L	CALEDQ 23,297,1984
Unscheduled DNA Synthesis	oral/rat	77 gm/kg/11W	TXAPA9 60,287,1981

Reproductive Effects Data and References

Route/Organism	Dose	Effect	Reference
inhalation/rat	15 ppm/6H (6-20D pregnant)	Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)	TOLED5 47,235,1989
intraperitoneal/rat	150 mg/kg (1-15D pregnant)	Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants) Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)	SWEHDO 7(Suppl 4),66,1981
oral/rat	45 mg/kg (13W male/13W pre-3W after birth)	Reproductive: Effects on newborn: Biochemical and metabolic	TXAPA9 42,387,1977
oral/rat	4 gm/kg (13W male/13W pre-3W after birth)	Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)	TXAPA9 42,387,1977
oral/rat	178 mg/kg (1-22D pregnant)	Reproductive: Specific developmental abnormalities: Central nervous system Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)	AAGEAA 89(8),44,1985
oral/mouse	10920 µg/kg (13W male)	Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)	NTIS** PB91-185884
oral/rat	20 mg/kg (multigenerations)	Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)	NTIS** #PB93-123867
oral/rat	75 mg/kg (multigenerations)	Reproductive: Specific developmental abnormalities: Urogenital system Reproductive: Effects on newborn: Biochemical and metabolic	NTIS** #PB93-123867
oral/rat	225 mg/kg (17-22D pregnant)	Reproductive: Effects on newborn: Live birth index (Litter size (e.g., # fetuses per litter; measured after birth)	NTIS** #PB93-123867
oral/rat	37.5 mg/kg (multigenerations)	Reproductive: Effects on newborn: Viability index (e.g., # alive at day 4 per # born alive) Reproductive: Effects on newborn: Biochemical and metabolic	NTIS** #PB93-123867
oral/rat	5 mg/kg (multigenerations)	Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)	NTIS** #PB93-123867
subcutaneous/rat	20 mg/kg (1D prior to copulation)	Reproductive: Effects on newborn: Weaning or lactation index (e.g., # alive at weaning per # alive at day 4)	GISAAA 31,33,1966

Tumorigenic Data and References

Route/Organism	Dose	Effect	Reference
oral/rat	lowest published toxic dose: 15 gm/kg/2Y- continuous	Tumorigenic: Carcinogenic by RTECS criteria Kidney, Ureter, and Bladder: Kidney tumors	AIHAAP 38,589,1977

Acute Toxicity Data and References

Route/Organism	Dose	Effect	Reference
In Vitro/Human, liver tumor	Inhibitor Concentration Low: 10 µmol/L	In Vitro Toxicity Studies: Other assays	TIVIEQ 26,511,2012
In Vitro/Rat, liver	Inhibitor Concentration Low: 31.6 µmol/L	In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc.	TIVIEQ 26,511,2012
inhalation/mammal (species unspecified)	lethal concentration (50 percent kill): 370 mg/m³		GISAAA 55(5),72,1990
inhalation/mouse	lethal concentration (50 percent kill): 370 mg/m³		GISAAA 37(2),32,1972
inhalation/mouse	lethal concentration (50 percent kill): 370 mg/m³/2H		VCVGH* -,518,1990
inhalation/rat	lethal concentration (50 percent kill): 630 mg/m³/4H		VCVGH* -,518,1990
intraperitoneal/mouse	lethal dose (50 percent kill): 76 mg/kg		EJTXAZ 7,247,1974
intraperitoneal/mouse	lowest published toxic dose: 25 mg/kg	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)	VCVGH* -,519,1990
intraperitoneal/rat	lowest published toxic dose: 300 mg/kg	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Other changes in urine composition	VCVGH* -,519,1990
intraperitoneal/rat	lethal dose (50 percent kill): 175 mg/kg	Behavioral: Somnolence (general depressed activity) Behavioral: Irritability	EJTXAZ 8,180,1975
intraperitoneal/rat	lowest published toxic dose: 50 mg/kg	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)	TOLED5 182,84,2008
intraperitoneal/rat	lowest published toxic dose: 100 mg/kg	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) Biochemical: Metabolism (intermediary): Other proteins	TOLED5 182,84,2008
intraperitoneal/rat	lowest published toxic dose: 100 mg/kg	Kidney, Ureter, and Bladder: Other changes	TOLED5 172S,S1,2007
intraperitoneal/rat	lowest published toxic dose: 100 mg/kg	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Renal function tests depressed	TOLED5 172S,S1,2007
oral/cat	lethal dose (50 percent kill): 187 mg/kg		VCVGH* -,519,1990
oral/guinea pig	lethal dose (50 percent kill): 90 mg/kg		ENTOX* -,513,2005
oral/guinea pig	lowest published toxic dose: 10 mg/kg	Nutritional and Gross Metabolic: Conditioned vitamin deficiency	VCVGH* -,519,1990
oral/hamster	lethal dose (50 percent kill): 960 mg/kg		TXAPA9 48,A192,1979
oral/mouse	lethal dose (50 percent kill): 51 mg/kg		VCVGH* -,518,1990
oral/rat	lethal dose (50 percent kill): 82 mg/kg		GISAAA 37(2),32,1972
oral/rat	lowest published toxic dose: 8.1 mg/kg	Spinal Cord: Other degenerative changes	VCVGH* -,520,1990
oral/rat	lowest published toxic dose: 10 mg/kg	Nutritional and Gross Metabolic: Conditioned vitamin deficiency	VCVGH* -,519,1990
oral/rat	lowest published toxic dose: 200 mg/kg	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Other changes in urine composition Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Peptidases	TXAPA9 132,203,1995
skin/mouse	lethal dose (50 percent kill): 150 mg/kg		VCVGH* -,518,1990
skin/rabbit	lethal dose (50 percent kill): 100 mg/kg		GISAAA 37(2),32,1972
skin/rabbit	lethal dose (50 percent kill): 126 mg/kg/7H		ENTOX* -,513,2005
skin/rat	lethal dose (50 percent kill): 4500 mg/kg		VCVGH* -,519,1990
unreported route/mammal (species unspecified)	lethal dose (50 percent kill): 200 mg/kg		30ZDA9 -,40,1971
unreported route/rat	lowest published toxic dose: 200 mg/kg	Kidney, Ureter, and Bladder: Other changes in urine composition	TOXID9 44,104,2005

Other Multiple Dose Data and References

Route/Organism	Dose	Effect	Reference
inhalation/man	lowest published toxic concentration: 0.02 ppm/9Y- intermittent	Liver: Change in gall bladder structure or function Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)	BJIMAG 49,700,1992
inhalation/rat	lowest published toxic concentration: 26 mg/m³/2H/30D- intermittent	Blood: Other changes Blood: Changes in leukocyte (WBC) count Nutritional and Gross Metabolic: Weight loss or decreased weight gain	GISAAA 37(2),32,1972
inhalation/rat	lowest published toxic concentration: 100 ppm/6H/2W- intermittent	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Nutritional and Gross Metabolic: Weight loss or decreased weight gain Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	BJIMAG 27,1,1970
intraperitoneal/rat	lowest published toxic dose: 50 mg/kg/2D- intermittent	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Renal function tests depressed Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases	CBCPAI 136,367,2003
intraperitoneal/rat	lowest published toxic dose: 75 mg/kg/3D- intermittent	Behavioral: Tolerance Kidney, Ureter, and Bladder: Other changes Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	CBCPAI 136,367,2003
intraperitoneal/rat	lowest published toxic dose: 100 mg/kg/4D- intermittent	Behavioral: Ataxia Behavioral: Tolerance Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	CBCPAI 136,367,2003
intraperitoneal/rat	lowest published toxic dose: 313 mg/kg/3D- intermittent	Liver: Other changes Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Phosphatases	TIHEEC 8,191,1992
oral/guinea pig	lowest published toxic dose: 420 mg/kg/30W- intermittent	Liver: Other changes Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) Immunological Including Allergic: Increase in cellular immune response	GTPZAB 9(11),50,1965
oral/mouse	lowest published toxic dose: 540 mg/kg/15D- continuous	Cardiac: Changes in heart weight Endocrine: Changes in spleen weight Nutritional and Gross Metabolic: Weight loss or decreased weight gain	JEPOEC 9,323,1989
oral/mouse	lowest published toxic dose: 1092 mg/kg/13W- continuous	Cardiac: Changes in heart weight Kidney, Ureter, and Bladder: Changes in bladder weight Nutritional and Gross Metabolic: Weight loss or decreased weight gain	NTIS** PB91-185884
oral/rat	lowest published toxic dose: 1274 mg/kg/26W- intermittent	Brain and Coverings: Other degenerative changes Behavioral: Alteration of operant conditioning Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)	GTPZAB 11(3),23,1967
oral/rat	lowest published toxic dose: 14600 mg/kg/2Y- continuous	Kidney, Ureter, and Bladder: Other changes in urine composition Kidney, Ureter, and Bladder: Other changes Nutritional and Gross Metabolic: Weight loss or decreased weight gain	EVHPAZ 21,49,1977
oral/rat	lowest published toxic dose: 105 mg/kg/14D- continuous	Kidney, Ureter, and Bladder: Changes in bladder weight Nutritional and Gross Metabolic: Weight loss or decreased weight gain	TXAPA9 47,1,1979
oral/rat	lowest published toxic dose: 794 mg/kg/18W- intermittent	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Changes in bladder weight Liver: Changes in liver weight	TXAPA9 47,1,1979
oral/rat	lowest published toxic dose: 80 mg/kg/16D- continuous	Behavioral: Food intake (animal) Kidney, Ureter, and Bladder: Other changes Nutritional and Gross Metabolic: Weight loss or decreased weight gain	NTIS** #PB93-123867
oral/rat	lowest published toxic dose: 160 mg/kg/16D- continuous	Behavioral: Tremor Behavioral: Muscle weakness	NTIS** #PB93-123867
oral/rat	lowest published toxic dose: 160 mg/kg/16D- continuous	Behavioral: Sleep	NTIS** #PB93-123867
oral/rat	lowest published toxic dose: 160 mg/kg/16D- continuous	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Changes in kidney weight	NTIS** #PB93-123867
oral/rat	lowest published toxic dose: 1040 mg/kg/16D- continuous	Kidney, Ureter, and Bladder: Other changes in urine composition	NTIS** #PB93-123867
oral/rat	lowest published toxic dose: 320 mg/kg/16D- continuous	Behavioral: Fluid intake	NTIS** #PB93-123867
oral/rat	lowest published toxic dose: 600 mg/kg/16D- continuous	Kidney, Ureter, and Bladder: Urine volume increased	NTIS** #PB93-123867
oral/rat	lowest published toxic dose: 385 mg/kg/7D- continuous	Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	NTIS** #PB93-123867
oral/rat	lowest published toxic dose: 560 mg/kg/4W- continuous	Kidney, Ureter, and Bladder: Other changes in urine composition Endocrine: Changes in adrenal weight Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases	FCTOD7 31,125,1993

Reviews

Organization	Standard	Reference
American Conference of Governmental Industrial Hygienists (ACGIH)	Threshold Limit Value-time-weighted average 0.02 ppm (skin)	DTLVS* TLV/BEI,2013
American Conference of Governmental Industrial Hygienists (ACGIH)	Threshold Limit Value-Confirmed animal carcinogen	DTLVS* TLV/BEI,2013
International Agency for Research on Cancer (IARC)	Cancer Review:Animal Limited Evidence	IMEMDT 20,179,1979
International Agency for Research on Cancer (IARC)	Cancer Review:Animal Limited Evidence	IMEMDT 73,277,1999
International Agency for Research on Cancer (IARC)	Cancer Review:Human No Adequate Data	IMEMDT 20,179,1979
International Agency for Research on Cancer (IARC)	Cancer Review:Human Inadequate Evidence	IMEMDT 73,277,1999
International Agency for Research on Cancer (IARC)	Cancer Review:Group 3	IMEMDT 73,277,1999
TOXICOLOGY REVIEW		ENTOX* -,513,2005
TOXICOLOGY REVIEW		MUREAV 584,1,2005
TOXICOLOGY REVIEW		BCLPT* 96,131,2005
TOXICOLOGY REVIEW		MUREAV 590,1,2005
TOXICOLOGY REVIEW		MUREAV 802,1,2016

Standards and Regulations

Organization	Standard	Reference
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV;
Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV
Occupational Exposure Limit-AUSTRALIA	time-weighted average 0.02 ppm (0.21 mg/m³), Carcinogen, JUL2008
Occupational Exposure Limit-BELGIUM	time-weighted average 0.02 ppm (0.21 mg/m³), Skin, MAR2002
Occupational Exposure Limit-DENMARK	time-weighted average 0.02 ppm (0.24 mg/m³), skin, MAY2011
Occupational Exposure Limit-ICELAND	time-weighted average 0.02 ppm (0.24 mg/m³), skin, NOV2011
Occupational Exposure Limit-KOREA	time-weighted average 0.02 ppm (0.24 mg/m³), skin, 2006
Occupational Exposure Limit-NEW ZEALAND	time-weighted average 0.02 ppm (0.21 mg/m³), skin, JAN2002
Occupational Exposure Limit-NORWAY	time-weighted average 0.02 ppm (0.24 mg/m³), JAN1999
Occupational Exposure Limit-PERU	time-weighted average 0,02 ppm (0,21 mg/m³), JUL2005
Occupational Exposure Limit-RUSSIA	short term exposure limit 0.005 mg/m³, Skin, JUN2003
Occupational Exposure Limit-SWITZERLAND	MAK-week 0.02 ppm (0.24 mg/m³), carc 3, skin, JAN2011
Occupational Exposure Limit-THE NETHERLANDS	MAC-TGG 0.24 mg/m³, 2003

NIOSH Documentation and Surveillance

Organization	Standard	Reference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO HEXACHLOROBUTADIENE-air	10H Carcinogen time-weighted average 0.02 ppm (Sk)	NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983	Hazard Code X6278; Number of Industries 1; Total Number of Facilities 3; Number of Occupations 11; Total Number of Employees Exposed 1010; Total Number of Female Employees Exposed 26

Status in Federal Agencies

Organization	Reference
ATSDR TOXICOLOGY PROFILE (NTIS** PB/95/100160/AS)
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULE	FEREAC 47,26992,82
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NIOSH Analytical Method, 1994: Hexachlorobutadiene, 2543
NTP Toxicity studies, RPT# TOX-01, October 2000
On EPA IRIS database

Page last reviewed: November 16, 2018

Content source: National Institute for Occupational Safety and Health