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Toluene - 2,4 - diamine

RTECS #: XS9625000

CAS #: 95-80-7


UPDATE: February 2009 MW: 122.19 MF: C7H10N2


NOTE:

TABLE OF CONTENTS:
  1. SYNONYMS:
  2. SKIN AND EYE IRRITATION DATA:
  3. MUTATION DATA:
  4. REPRODUCTIVE EFFECTS DATA:
  5. TUMORIGENIC DATA:
  6. ACUTE TOXICITY DATA:
  7. OTHER MULTIPLE DOSE DATA:
  8. REVIEWS:
  9. STANDARDS AND REGULATIONS:
  10. NIOSH DOCUMENTATION AND SURVEILLANCE:
  11. STATUS IN FEDERAL AGENCIES:
  12. REFERENCES:

SYNONYMS:

  1. 1,3 - Benzenediamine, 4 - methyl -
  2. 1,3 - Diamino - 4 - methylbenzene
  3. 2,4 - Diamino - 1 - methylbenzene
  4. 2,4 - Diaminotoluen (Czech)
  5. 2,4 - Diaminotoluene
  6. 2,4 - Diamino - 1 - toluene
  7. 2,4 - Diaminotoluol
  8. 2,4 - Tolamine
  9. 2,4 - Tolylenediamine
  10. 3 - Amino - p - toluidine
  11. 4 - Methyl - 1,3 - benzenediamine
  12. 4 - Methyl - m - phenylenediamine
  13. 5 - Amino - o - toluidine
  14. Benzofur MT
  15. Brown for Fur T
  16. C.I. 76035
  17. C.I. Oxidation Base 20
  18. C.I. Oxidation Base 35
  19. C.I. Oxidation Base 200
  20. Developer 14
  21. Developer B
  22. Developer DB
  23. Developer DBJ
  24. Developer MC
  25. Developer MT
  1. Developer MT - CF
  2. Developer MTD
  3. Developer T
  4. Eucanine GB
  5. Fouramine
  6. Fouramine J
  7. Fourrine 94
  8. Fourrine M
  9. MTD
  10. NCI - C02302
  11. Nako TMT
  12. Pelagol Grey J
  13. Pelagol J
  14. Pontamine developer TN
  15. RCRA waste number U221
  16. Renal MD
  17. TDA
  18. Tertral G
  19. Tolylene - 2,4 - diamine
  20. Zoba GKE
  21. m - Toluenediamine
  22. m - Toluylendiamin (Czech)
  23. m - Toluylenediamine
  24. m - Tolyenediamine
  25. m - Tolylenediamine


SKIN AND EYE IRRITATION DATA AND REFERENCES:

ROUTE/
ORGANISM
DOSE
EFFECT

REFERENCE
eye
rabbit
100 mg/24 hour moderate 85JCAE -,477,1986
eye
rabbit
100 mg/24 hour moderate TAEHC* IP:74,1,1987
skin
rabbit
500 mg/24 hour mild 85JCAE -,477,1986
skin
rabbit
500 mg/24 hour mild TAEHC* IP:75,1,1987


MUTATION DATA AND REFERENCES:

SYSTEM TEST ROUTE/
ORGANISM/
TISSUE
DOSE REFERENCE
body fluid assay rat
Salmonella typhimurium
70 mg/kg JJIND8 76,291,1986
cytogenetic analysis hamster ovary 4 mmol/L MUREAV 265,45,1992
DNA adduct oral
rat
84 mg/kg/3 week- continuous MUREAV 367,209,1996
DNA damage human fibroblast 100 mol/L MUREAV 127,107,1984
DNA damage human mammary gland 98 mg/L/24 hour (-enzymatic activation step) MUREAV 588,47,2005
DNA damage human other cell types 1.46 mmol/L/20 hour TXCYAC 213,138,2005
DNA damage human liver 1.46 mmol/L/20 hour TXCYAC 213,138,2005
DNA damage intraperitoneal
mouse
240 mg/kg MUREAV 391,201,1997
DNA damage oral
mouse
60 mg/kg MUREAV 440,1,1999
DNA damage intraperitoneal
rat
37 mg/kg CRNGDP 6,1285,1985
DNA inhibition intraperitoneal
mouse
111 mg/kg MUREAV 91,75,1981
unscheduled DNA synthesis human liver 100 mol/L CNREA8 49,1075,1989
unscheduled DNA synthesis human other cell types 0.1 mmol/L/20 hour TXCYAC 213,138,2005
unscheduled DNA synthesis oral
rat
150 mg/kg ENMUDM 4,553,1982
unscheduled DNA synthesis rat liver 10 mol/L MUREAV 315,147,1994
mutation in microorganisms Salmonella typhimurium 10 mg/plate (+enzymatic activation step) ENMUDM 5(Suppl 1),3,1983
mutation in microorganisms Salmonella typhimurium 10 g/plate (-enzymatic activation step) JJIND8 76,291,1986
mutation in microorganisms Salmonella typhimurium 625 g/plate/20 minute (+enzymatic activation step) MUTAEX 20,217,2005
mutation in microorganisms Salmonella typhimurium 2,500 g/plate/48 hour (+enzymatic activation step) MUTAEX 20,217,2005
micronucleus test human other cell types 2.14 mmol/L/20 hour TXCYAC 213,138,2005
micronucleus test human liver 2.14 mmol/L/20 hour TXCYAC 213,138,2005
micronucleus test oral
rat
300 mg/kg CRNGDP 12,2233,1991
mutation in mammalian somatic cells hamster ovary 2 gm/L MUREAV 135,115,1984
mutation in mammalian somatic cells mouse lymphocyte 198 mg/L MUREAV 135,115,1984
morphological transform hamster embryo 500 g/L CALEDQ 3,45,1977
morphological transform hamster embryo 50 mg/L/24 hour MUREAV 577S,1,2005
morphological transform hamster embryo 10 mg/L/7 day MUREAV 654,108,2008
morphological transform mouse fibroblast 10 mg/L/21 day (-enzymatic activation step) EMMUEG 35,300,2000
other mutation test systems hamster ovary 6 mmol/L MUREAV 265,45,1992
other mutation test systems Salmonella typhimurium 60 mg/L MUREAV 192,239,1987
phage inhibition capacity Escherichia coli 100 mmol/L MDMIAZ 31,11,1979
sister chromatid exchange intraperitoneal
mouse
9 mg/kg MUREAV 108,225,1983
sex chromosome loss and nondisjunction parenteral
Drosophila melanogaster
5 mmol/L MUREAV 56,31,1977
sex chromosome loss and nondisjunction oral
Drosophila melanogaster
15,200 mmol/L/3 day MUREAV 48,181,1977
specific locus test skin
mouse
5,600 mg/kg/28 day- continuous MUREAV 608,88,2006


REPRODUCTIVE EFFECTS DATA AND REFERENCES:

ROUTE/
ORGANISM
DOSE
EFFECT

REFERENCE
oral
mouse
lowest published toxic dose: 1,200 mg/kg (6-13 day pregnant) Reproductive: Effects on fertility: Litter size (e.g., # fetuses per litter; measured before birth)

Reproductive: Effects on newborn: Live birth index (Litter size (e.g., # fetuses per litter; measured after birth)
TCMUD8 7,29,1987
oral
rat
lowest published toxic dose: 1,260 mg/kg (10 week male) Reproductive: Effects on fertility: Mating performance (e.g., # sperm positive females per # females mated; # copulations per # estrus cycles) JTEHD6 16,753,1985
oral
rat
lowest published toxic dose: 1,260 mg/kg (10 week male) Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Prostate, seminal vesicle, Cowper's gland, accessory glands
JTEHD6 16,763,1985
oral
rat
lowest published toxic dose: 210 mg/kg (1 week male) Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct
JTEHD6 25,435,1988


TUMORIGENIC DATA AND REFERENCES:

ROUTE/
ORGANISM
DOSE
EFFECT

REFERENCE
oral
mouse
lowest published toxic dose: 8,050 mg/kg/101 week- continuous Tumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Blood: Lymphoma including Hodgkin's disease
NCITR* NCI-TR-162,1979
oral
mouse
toxic dose : 17 gm/kg/2 year- continuous Tumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Blood: Lymphoma including Hodgkin's disease
NCITR* NCI-TR-162,1979
oral
mouse
toxic dose : 8,400 mg/kg/2 year- continuous Tumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
GANNA2 70,453,1979
oral
mouse
toxic dose : 16,800 mg/kg/2 year- continuous Tumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
GANNA2 70,453,1979
oral
rat
lowest published toxic dose: 2,100 mg/kg/90 week- continuous Tumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Skin and Appendages: Tumors
JJIND8 62,1107,1979
oral
rat
toxic dose : 5,030 mg/kg/84 week- continuous Tumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors

Blood: Lymphoma including Hodgkin's disease
NCITR* NCI-TR-162,1979
oral
rat
toxic dose : 9,000 mg/kg/36 week- continuous Tumorigenic: Carcinogenic by RTECS criteria

Liver: Tumors
CNREA8 29,1137,1969


ACUTE TOXICITY DATA AND REFERENCES:

ROUTE/
ORGANISM
DOSE
EFFECT

REFERENCE
intraperitoneal
mouse
lethal dose (50 percent kill): 480 mg/kg N/R JEPTDQ 3(1-2),149,1979
intraperitoneal
rat
lethal dose (50 percent kill): 325 mg/kg N/R JEPTDQ 3(1-2),149,1979
oral
rat
lethal dose (50 percent kill): 590 mg/kg/24 hour N/R TAEHC* -,1,1987
subcutaneous
dog
lowest published lethal dose: 200 mg/kg N/R XPHBAO 271,56,1941
subcutaneous
rat
lowest published lethal dose: 350 mg/kg Behavioral: Somnolence (general depressed activity)

Cardiac: Pulse rate decreased with fall in BP

Lung, Thorax, or Respiration: Respiratory stimulation
ZEPTAT 17,59,1915
subcutaneous
rabbit
lowest published lethal dose: 400 mg/kg Brain and Coverings: Other degenerative changes

Behavioral: Convulsions or effect on seizure threshold

Lung, Thorax, or Respiration: Respiratory stimulation
ZEPTAT 17,59,1915
skin
rabbit
lethal dose (50 percent kill): 650 mg/kg/24 hour N/R TAEHC* -,1,1987


OTHER MULTIPLE DOSE DATA AND REFERENCES:

ROUTE/
ORGANISM
DOSE
EFFECT

REFERENCE
oral
mouse
lowest published toxic dose: 700 mg/kg/7 day- intermittent Nutritional and Gross Metabolic: Weight loss or decreased weight gain NTPTR* IMM-87034
oral
mouse
lowest published toxic dose: 1,400 mg/kg/14 day- intermittent Liver: Hepatitis (hepatocellular necrosis), zonal

Liver: Changes in liver weight

Endocrine: Changes in spleen weight
NTPTR* IMM-87034
oral
mouse
lowest published toxic dose: 1,400 mg/kg/14 day- intermittent Kidney, Ureter, and Bladder: Renal function tests depressed

Blood: Changes in other cell count (unspecified)

Blood: Changes in leukocyte (WBC) count
NTPTR* IMM-87034
oral
mouse
lowest published toxic dose: 1,400 mg/kg/14 day- intermittent Immunological Including Allergic: Increase in cellular immune response

Immunological Including Allergic: Decrease in cellular immune response

Immunological Including Allergic: Decreased immune response
NTPTR* IMM-87034
oral
mouse
lowest published toxic dose: 1,400 mg/kg/14 day- intermittent Immunological Including Allergic: Uncharacterized (multiple organ involvement)

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NTPTR* IMM-87034
oral
mouse
lowest published toxic dose: 700 mg/kg/14 day- intermittent Immunological Including Allergic: Increase in cellular immune response NTPTR* IMM-87034
oral
rat
lowest published toxic dose: 36,500 mg/kg/2 year- intermittent Kidney, Ureter, and Bladder: Other changes

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
TAEHC* -,1,1987
oral
rat
lowest published toxic dose: 1,050 mg/kg/70 day - continuous Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct

Reproductive: Paternal effects: Prostate, seminal vesicle, Cowper's gland, accessory glands
TAEHC* IP:74,67,1987
oral
rat
lowest published toxic dose: 350 mg/kg/5 day- intermittent Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects TAEHC* -,1,1987
oral
rat
lowest published toxic dose: 732.48 mg/kg/16 week- continuous Liver: Tumors

Liver: Changes in liver weight
TOXID9 78,367,2004
oral
rat
lowest published toxic dose: 2,197.44 mg/kg/16 week- continuous Liver: Multiple effects

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
TOXID9 78,367,2004


REVIEWS:

ORGANIZATION STANDARD
REFERENCE

International Agency for Research on Cancer (IARC) Cancer Review Animal Sufficient Evidence IMEMDT 16,83,1978
International Agency for Research on Cancer (IARC) Cancer Review Human No Adequate Data IMEMDT 16,83,1978
International Agency for Research on Cancer (IARC) Cancer Review Group 2B IMSUDL 7,56,1987
TOXICOLOGY REVIEW
MUREAV 543,201,2003
TOXICOLOGY REVIEW
MUREAV 567,227,2004
TOXICOLOGY REVIEW
EMMUEG 43,36,2004
TOXICOLOGY REVIEW
MUREAV 588,88,2005
TOXICOLOGY REVIEW
MUREAV 627,10,2007
TOXICOLOGY REVIEW
MUREAV 654,114,2008
TOXICOLOGY REVIEW
MUREAV 654,114,2008


STANDARDS AND REGULATIONS:

ORGANIZATION STANDARD
REFERENCE

Occupational Exposure Limit - AUSTRIA TRK 0.1 mg/m3, JAN 2006
Occupational Exposure Limit - FINLAND Carcinogen, JAN1999
Occupational Exposure Limit - POLAND MAC(time-weighted average) 0.04 mg/m3, MAC(short term exposure limit) 0.1 mg/m3, JAN1999
Occupational Exposure Limit - RUSSIA short term exposure limit 2 mg/m3, Skin, JUN2003
Occupational Exposure Limit - SWEDEN Group B Carcinogen, Sen, JUN2005
Occupational Exposure Limit - SWITZERLAND MAK- week 0.02 ppm (0.1 mg/m3), Skin, DEC2006


NIOSH DOCUMENTATION AND SURVEILLANCE:

ORGANIZATION STANDARD or SURVEY
REFERENCE

National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO TOLUENEDIAMINE-air Carcinogen lowest feasible concentration NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983 National Occupational Exposure Survey 1983: Hazard Code: X5386;
Number of Industries 6;
Total Number of Facilities 130;
Number of Occupations 15;
Total Number of Employees Exposed 8,511;
Total Number of Female Employees Exposed 396


STATUS IN FEDERAL AGENCIES:

ORGANIZATION
REFERENCE

EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat; Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Negative: N crassa-aneuploidy; Sperm morphology-mouse
EPA GENETOX PROGRAM 1988, Inconclusive: SHE-clonal assay
EPA TSCA Section 8(b) CHEMICAL INVENTORY
Used as an intermediate in the production of POLYURETHANE, and to make dyes for textiles, fur
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, PROPOSED RULE FEREAC 47,27009,82
EPA TSCA Section 8(d) unpublished health/safety studies
On EPA IRIS database
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NIOSH CURRENT INTELLIGENCE BULLETIN 53, DECEMBER 1989
NIOSH Analytical Method, 1994: 2,4-Toluenediamine, 5516
NCI Carcinogenesis Bioassay (feed);clear evidence:mouse,rat NCITR* NCI-TR-162,1979
NTP 11th Report on Carcinogens,2004:Reasonably anticipated to be a human carcinogen
NTP Carcinogenesis studies; test completed (peer review), October 2000
OSHA ANALYTICAL METHOD #ID-65


REFERENCES:

CODEN
REFERENCE

85JCAE "Prehled Prumyslove Toxikologie; Organicke Latky," Marhold, J., Prague, Czechoslovakia, Avicenum, 1986
CALEDQ Cancer Letters (Shannon, Ireland). (Elsevier Scientific Pub. Ireland Ltd., POB 85, Limerick, Ireland) V.1- 1975-
CNREA8 Cancer Research. (Public Ledger Building, Suit 816, 6th & Chestnut Sts., Philadelphia, PA 19106) V.1- 1941-
CRNGDP Carcinogenesis (London). (Oxford Univ. Press, Pinkhill House, Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1980-
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.10- 1987-
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG.
FEREAC Federal Register. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402) V.1- 1936-
GANNA2 Gann. Japanese Journal of Cancer Research. (Tokyo, Japan) V.1-75, 1907-84. For publisher information, see JJCREP.
IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972-
IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) No.1- 1979-
JEPTDQ Journal of Environmental Pathology and Toxicology. (Park Forest South, IL) V.1-5(3), 1977-81(?). For publisher information, see JEPOEC.
JJIND8 JNCI, Journal of the National Cancer Institute. (Washington, DC) V.61-79, 1978-87. For publisher information, see JNCIEQ.
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub., 1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
MDMIAZ Medycyna Doswiadczalna i Mikrobiologia. For English translation, see EXMMAV. (Ars Polona, POB 1001, 00-068 Warsaw 1, Poland) V.1- 1949-
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964-
MUTAEX Mutagenesis. (Oxford Univ. Press, Pinkhill House, Southfield Road, Eynsham, Oxford OX8 1JJ, UK) V.1- 1986-
NCITR* National Cancer Institute Carcinogenesis Technical Report Series. (Bethesda, MD) No.0-205. For publisher information, see NTPTR*.
NIOSH* National Institute of Occupational Safety and Health, U.S. Dept. of Health, Education, and Welfare, Reports and Memoranda.
NTPTR* National Toxicology Program Technical Report Series. (Research Triangle Park, NC 27709) No.206-
TAEHC* Environmental Health Criteria (United Nations Environment Programme. Geneva, World Health Organization) 1- 1976-
TCMUD8 Teratogenesis, Carcinogenesis, and Mutagenesis. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.1- 1980-
TOXID9 Toxicologist. (Soc. of Toxicology, Inc., 475 Wolf Ledge Parkway, Akron, OH 44311) V.1- 1981-
TXCYAC Toxicology. (Elsevier Scientific Pub. Ireland, Ltd., POB 85, Limerick, Ireland) V.1- 1973-
XPHBAO U.S. Public Health Service, Public Health Bulletin. (Washington, DC)
ZEPTAT Zeitschrift fuer Experimentelle Pathologie und Therapie. (Berlin, Ger.) V.1-22, 1905-21. For publisher information, see REXMAS.

Used as an intermediate in the production of POLYURETHANE, and to make dyes for textiles, fur

RTECS Compound Description:
   Agricultural Chemical
   Tumorigen
   Mutagen
   Reproductive Effector
   Human Data
   Primary Irritant

Click Here for Additional Information about RTECS

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