Methane, dichloro-
RTECS #
PA8050000
CAS #
Updated
March 2019
Molecular Weight
84.93
Molecular Formula
CH2Cl2
Synonyms
Aerothene MM
Chlorure de methylene (French)
Dichloromethane
Dichloromethane (DOT:OSHA)
F 30 (chlorocarbon)
Freon 30
HCC 30
Khladon 30
Methane dichloride
Methylene bichloride
Methylene chloride
Methylene chloride (ACGIH:OSHA)
Methylene dichloride
Metylenu chlorek (Polish)
Narkotil
NCI-C50102
R 30
R30 (refrigerant)
RCRA waste number U080
Solaesthin
Soleana VDA
Solmethine
Chlorure de methylene (French)
Dichloromethane
Dichloromethane (DOT:OSHA)
F 30 (chlorocarbon)
Freon 30
HCC 30
Khladon 30
Methane dichloride
Methylene bichloride
Methylene chloride
Methylene chloride (ACGIH:OSHA)
Methylene dichloride
Metylenu chlorek (Polish)
Narkotil
NCI-C50102
R 30
R30 (refrigerant)
RCRA waste number U080
Solaesthin
Soleana VDA
Solmethine
Skin and Eye Irritation and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| eye /rabbit | 162 mg | moderate | EJTXAZ 9,171,1976 |
| eye /rabbit | 10 mg | mild | TXCYAC 6,173,1976 |
| eye /rabbit | 500 mg/24H | mild | 85JCAE -,88,1986 |
| skin /rabbit | 810 mg/24H | severe | EJTXAZ 9,171,1976 |
| skin /rabbit | 100 mg/24H | moderate | 85JCAE -,88,1986 |
Mutation Data and Reference
| System Test | Route/Organism/Tissue | Dose | Reference |
|---|---|---|---|
| Cytogenetic Analysis | inhalation/mouse | 27760 mg/m3/6H/2W- intermittent | EMMUEG 15,221,1990 |
| Cytogenetic Analysis | lung/hamster | 1 µmol/L | TECSDY 13,205,1987 |
| Cytogenetic Analysis | ovary/hamster | 6628 mg/L | MUREAV 116,361,1983 |
| DNA Damage | ovary/hamster | 3000 ppm | MUREAV 367,143,1996 |
| DNA Damage | liver/mouse | 400 µmol/L | CRNGDP 15,991,1994 |
| DNA Damage | inhalation/mouse | 4000 ppm/6H-continuous | CRNGDP 15,991,1994 |
| DNA Damage | oral/mouse | 1720 mg/kg | MUREAV 419,13,1998 |
| DNA Damage | oral/rat | 1275 mg/kg | TCMUD8 9,61,1989 |
| DNA Damage | liver/rat | 30 µmol/L | CRNGDP 15,991,1994 |
| DNA Damage | lung/human | 1000 µmol/L/3H | MUREAV 538,41,2003 |
| DNA Damage | fibroblast/hamster | 5 mmol/L/2H | MUREAV 577S,1,2005 |
| DNA inhibition | fibroblast/human | 5000 ppm/1H-continuous | MUREAV 81,203,1981 |
| DNA inhibition | lung/hamster | 5000 ppm/1H-continuous | MUREAV 81,203,1981 |
| micronucleus test | inhalation/mouse | 27760 mg/m3/6H/2W- intermittent | EMMUEG 15,221,1990 |
| morphological transform | embryo/rat | 160 µmol/L | ITCSAF 14,290,1978 |
| morphological transform | embryo/hamster | 1300 µL/plate | EVSRBT 25,75,1982 |
| morphological transform | fibroblast/mouse | 0.1 mg/L/21D (-enzymatic activation step) | EMMUEG 35,300,2000 |
| mutation in mammalian somatic cells | ovary/hamster | 3000 ppm | MUREAV 367,143,1996 |
| mutation in microorganisms | /Salmonella typhimurium | 140 gm/L/48H | MUTAEX 32,455,2017 |
| mutation in microorganisms | /Salmonella typhimurium | 5700 ppm (+/-enzymatic activation step) | MUREAV 56,245,1978 |
| mutation in microorganisms | /Escherichia coli | 6300 ppm (+/-enzymatic activation step) | EMMUEG 20,211,1992 |
| other mutation test systems | ovary/hamster | 6628 mg/L | MUREAV 116,361,1983 |
| phage inhibition capacity | /Escherichia coli | 8250 µg/well | MUREAV 260,349,1991 |
| sex chromosome loss and nondisjunction | oral/Drosophila melanogaster | 125 mmol/L | MUREAV 90,91,1981 |
| sister chromatid exchange | lung/hamster | 5000 ppm/1H-continuous | MUREAV 81,203,1981 |
| sister chromatid exchange | inhalation/mouse | 13880 mg/m3/6H/2W- intermittent | EMMUEG 15,221,1990 |
| sister chromatid exchange | /human | 500 ppm/72H | EMMUEG 48,625,2007 |
| sister chromatid exchange | other cell types/human | 30 ppm/72H | TOLED5 199,218,2010 |
Reproductive Effects Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| inhalation/rat | 4500 ppm/24H (1-17D pregnant) | Reproductive: Effects on newborn: Behavioral | TXAPA9 52,29,1980 |
| inhalation/rat | 1250 ppm/7H (6-15D pregnant) | Reproductive: Specific developmental abnormalities: Musculoskeletal system Reproductive: Specific developmental abnormalities: Urogenital system | TXAPA9 32,84,1975 |
| inhalation/mouse | 1250 ppm/7H (6-15D pregnant) | Reproductive: Specific developmental abnormalities: Musculoskeletal system | TXAPA9 32,84,1975 |
Tumorigenic Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| inhalation/mouse | lowest published toxic concentration: 2000 ppm/5H/2Y- continuous | Tumorigenic: Carcinogenic by RTECS criteria Lung, Thorax, or Respiration: Tumors | NTPTR* NTP-TR-306,1986 |
| inhalation/mouse | lowest published toxic concentration: 122400 mg/kg/102W- intermittent | Tumorigenic: Carcinogenic by RTECS criteria Lung, Thorax, or Respiration: Tumors Liver: Tumors | NTIS** PB86-187903/AS |
| inhalation/mouse | lowest published toxic concentration: 30239.3 mg/kg/13W- intermittent | Tumorigenic: Equivocal tumorigenic agent by RTECS criteria Lung, Thorax, or Respiration: Tumors | MUREAV 746,135,2012 |
| inhalation/mouse | lowest published toxic concentration: 92337.7 mg/kg/13W- intermittent | Tumorigenic: Equivocal tumorigenic agent by RTECS criteria Lung, Thorax, or Respiration: Tumors | MUREAV 746,135,2012 |
| inhalation/rat | lowest published toxic concentration: 122400 mg/kg/103W- intermittent | Tumorigenic: Neoplastic by RTECS criteria Skin and Appendages: Tumors | NTIS** PB86-187903/AS |
| inhalation/rat | lowest published toxic concentration: 102000 mg/kg/102W- intermittent | Tumorigenic: Neoplastic by RTECS criteria Skin and Appendages: Tumors Blood: Leukemia | NTIS** PB86-187903/AS |
| inhalation/rat | lowest published toxic concentration: 3500 ppm/6H/2Y- intermittent | Tumorigenic: Carcinogenic by RTECS criteria Endocrine: Tumors | FAATDF 4,30,1984 |
Acute Toxicity Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| eye/rabbit | lowest published toxic dose: 0.25 mL/kg | Eye: Increased intraocular pressure Eye: Conjunctiva irritation Eye: Corneal damage | VCVGH* -,323,1990 |
| In Vitro/Chicken, neurons | Inhibitor Concentration (50 percent kill): 125548 µmol/L/21H | In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. | TIVIEQ 7,653,1993 |
| In Vitro/Chicken, neurons | Inhibitor Concentration (10 percent kill): 32249 µmol/L/21H | In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. | TIVIEQ 7,653,1993 |
| In Vitro/Chicken, neurons | Inhibitor Concentration (50 percent kill): 87758 µmol/L/20H | In Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc. | TIVIEQ 7,653,1993 |
| In Vitro/Chicken, neurons | Inhibitor Concentration (10 percent kill): 25310 µmol/L/20H | In Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc. | TIVIEQ 7,653,1993 |
| In Vitro/Human, leukemia cells | Inhibitor Concentration (20 percent kill): 16.54 mmol/L/3H | In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. In Vitro Toxicity Studies: Other assays | TXCYAC 271,122,2010 |
| In Vitro/Human, leukemia cells | Inhibitor Concentration (50 percent kill): 53.86 mmol/L/3H | In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. In Vitro Toxicity Studies: Other assays | TXCYAC 271,122,2010 |
| inhalation/cat | Lowest published lethal concentration: 43400 mg/m3/4.5H | Behavioral: General anesthetic Behavioral: Ataxia Nutritional and Gross Metabolic: Body temperature decrease | AHBAAM 116,131,1936 |
| inhalation/Dog | Lowest published lethal concentration: 14108 ppm/7H | Eye: Miosis (pupilliary constriction) Vascular: BP lowering not characterized in autonomic section Lung, Thorax, or Respiration: Dyspnea | NIHBAZ 191,1,1949 |
| inhalation/guinea pig | Lowest published lethal concentration: 5000 ppm/2H | FLCRAP 1,197,1967 | |
| inhalation/guinea pig | lethal concentration (50 percent kill): 40200 mg/m3/6H | VCVGH* -,320,1990 | |
| inhalation/guinea pig | lowest published toxic concentration: 21000 mg/m3/45M | Behavioral: General anesthetic | VCVGH* -,320,1990 |
| inhalation/human | lowest published toxic concentration: 69600 mg/m3/30M | Behavioral: General anesthetic | VCVGH* -,321,1990 |
| inhalation/human | lowest published toxic concentration: 25000 mg/m3/8M | Peripheral Nerve and Sensation: Paresthesia Eye: Conjunctiva irritation | VCVGH* -,321,1990 |
| inhalation/human | lowest published toxic concentration: 8000 mg/m3/30M | Gastrointestinal: Nausea or vomiting | VCVGH* -,321,1990 |
| inhalation/human | lowest published toxic concentration: 500 ppm/1Y- intermittent | Behavioral: Altered sleep time (including change in righting reflex) Behavioral: Somnolence (general depressed activity) Cardiac: Change in rate | ABHYAE 43,1123,1968 |
| inhalation/human | lowest published toxic concentration: 500 ppm/8H | Behavioral: Euphoria | SCIEAS 176,295,1972 |
| inhalation/mouse | lethal concentration (50 percent kill): 14400 ppm/7H | NIHBAZ 191,1,1949 | |
| inhalation/mouse | lowest published toxic concentration: 32500 mg/m3/2H | Behavioral: General anesthetic | VCVGH* -,307,1990 |
| inhalation/mouse | lethal concentration (50 percent kill): 49100 mg/m3/6H | VCVGH* -,320,1990 | |
| inhalation/mouse | lethal concentration (16 percent kill): 53000 mg/m3/6H | VCVGH* -,320,1990 | |
| inhalation/mouse | lethal concentration (50 percent kill): 54000 mg/m3/2H | VCVGH* -,320,1990 | |
| inhalation/mouse | lethal concentration (84 percent kill): 75000 mg/m3/2H | VCVGH* -,320,1990 | |
| inhalation/mouse | lethal concentration (50 percent kill): 56220 mg/m3/7H | VCVGH* -,320,1990 | |
| inhalation/mouse | lowest published toxic concentration: 35 gm/m3/2H | Brain and Coverings: Changes in circulation Brain and Coverings: Other degenerative changes Behavioral: General anesthetic | TPKVAL 1,96,1961 |
| inhalation/mouse | lowest published toxic concentration: 15 gm/m3/2H | Behavioral: General anesthetic Behavioral: Convulsions or effect on seizure threshold Behavioral: Ataxia | TPKVAL 1,96,1961 |
| inhalation/mouse | lethal concentration (50 percent kill): 25 gm/m3/2H | Behavioral: Alteration of classical conditioning Liver: Fatty liver degeneration Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) | TPKVAL 1,96,1961 |
| inhalation/mouse | lowest published toxic concentration: 10 gm/m3/2H | TPKVAL 1,96,1961 | |
| inhalation/mouse | lowest published toxic concentration: 40000 mg/m3 | Behavioral: General anesthetic | VCVGH* -,320,1990 |
| inhalation/mouse | lowest published toxic concentration: 17000 mg/m3/2H | Behavioral: Somnolence (general depressed activity) | VCVGH* -,320,1990 |
| inhalation/mouse | Lowest published lethal concentration: 30 gm/m3/2H | Skin: After systemic exposure: Dermatitis, other | TPKVAL 1,96,1961 |
| inhalation/mouse | Lowest published lethal concentration: 50 gm/m3/2H | Behavioral: Ataxia Lung, Thorax, or Respiration: Respiratory obstruction Liver: Fatty liver degeneration | TPKVAL 1,96,1961 |
| inhalation/rabbit | Lowest published lethal concentration: 10000 ppm/7H | JIHTAB 26,8,1944 | |
| inhalation/rat | lethal concentration (50 percent kill): 76000 mg/m3/4H | VCVGH* -,320,1990 | |
| inhalation/rat | lethal concentration (50 percent kill): 52000 mg/m3/6H | VCVGH* -,320,1990 | |
| inhalation/rat | Lowest published lethal concentration: 52000 mg/m3 | Blood: Other changes Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other enzymes | TPKVAL 15,64,1979 |
| inhalation/rat | lowest published toxic concentration: 1000 mg/m3/30M | Endocrine: Adrenal cortex hyperplasia Behavioral: Alteration of classical conditioning | VCVGH* -,320,1990 |
| inhalation/rat | lowest published toxic concentration: 50000 mg/m3 | Behavioral: General anesthetic | VCVGH* -,320,1990 |
| inhalation/rat | lowest published toxic concentration: 1 gm/m3/1H | TPKVAL 1,96,1961 | |
| intraperitoneal/Dog | lethal dose (50 percent kill): 1274 mg/kg | TXAPA9 10,119,1967 | |
| intraperitoneal/mouse | lethal dose (50 percent kill): 437 mg/kg | AGGHAR 18,109,1960 | |
| intraperitoneal/mouse | lowest published toxic dose: 509.58 mg/kg | Blood: Methemoglobinemia- Carboxhemoglobinemia | JAPTO* 27,25,2007 |
| intraperitoneal/rat | lowest published toxic dose: 510 mg/kg | Blood: Methemoglobinemia- Carboxhemoglobinemia | VCVGH* -,320,1990 |
| intraperitoneal/rat | lethal dose (50 percent kill): 916 mg/kg | ENVRAL 40,411,1986 | |
| intratracheal/rat | lowest published lethal dose: 350 mg/kg | Lung, Thorax, or Respiration: Structural or functional change in trachea or bronchi Lung, Thorax, or Respiration: Acute pulmonary edema | NTIS** OTS0520615 |
| intravenous/Dog | lowest published lethal dose: 200 mg/kg | QJPPAL 7,205,1934 | |
| intravenous/mouse | lowest published toxic dose: 133 mg/kg | Kidney, Ureter, and Bladder: Other changes | VCVGH* -,320,1990 |
| intravenous/rat | lowest published toxic dose: 1000 mg/kg | Liver: Other changes Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Cytochrome oxidases (indlucing oxidative phosphrylation) | VCVGH* -,320,1990 |
| oral/Dog | lowest published lethal dose: 3 gm/kg | QJPPAL 7,205,1934 | |
| oral/Dog | lethal dose (50 percent kill): 3000 mg/kg | VCVGH* -,320,1990 | |
| oral/human | lowest published lethal dose: 357 mg/kg | Peripheral Nerve and Sensation: Paresthesia Behavioral: Somnolence (general depressed activity) Behavioral: Convulsions or effect on seizure threshold | 34ZIAG -,390,1969 |
| oral/man | lowest published toxic dose: 1429 µL/kg | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Blood: Normocytic anemia Nutritional and Gross Metabolic: Changes in: Fe | YAKUD5 36,575,1994 |
| oral/mouse | lethal dose (50 percent kill): 873 mg/kg | EVHPAZ 106(Suppl 2),497,1998 | |
| oral/mouse | lethal dose (16 percent kill): 620 mg/kg | VCVGH* -,320,1990 | |
| oral/mouse | lethal dose (84 percent kill): 1650 mg/kg | VCVGH* -,320,1990 | |
| oral/rabbit | lethal dose (50 percent kill): 2000 mg/kg | VCVGH* -,320,1990 | |
| oral/rabbit | lowest published lethal dose: 1900 mg/kg | HBTXAC 1,94,1955 | |
| oral/rat | lethal dose (50 percent kill): 1600 mg/kg | Behavioral: Ataxia | FAONAU 48A,94,1970 |
| oral/rat | lethal dose (50 percent kill): 985 mg/kg | VCVGH* -,320,1990 | |
| oral/rat | lethal dose (16 percent kill): 800 mg/kg | VCVGH* -,320,1990 | |
| oral/rat | lethal dose (84 percent kill): 2200 mg/kg | VCVGH* -,320,1990 | |
| oral/rat | lowest published toxic dose: 237.8 mg/kg | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) | VCVGH* -,321,1990 |
| oral/rat | lowest published toxic dose: 160 mg/kg | Blood: Other changes | TXAPA9 250,162,2011 |
| subcutaneous/mouse | lethal dose (50 percent kill): 6460 mg/kg | TXAPA9 4,354,1962 | |
| subcutaneous/rabbit | lowest published lethal dose: 2700 mg/kg | QJPPAL 7,205,1934 | |
| unreported route/mouse | lethal dose (50 percent kill): 4770 mg/kg | Gastrointestinal: Ulceration or bleeding from stomach Gastrointestinal: Ulceration or bleeding from small intestine | ESKGA2 28,P31,1982 |
| unreported route/rabbit | lethal dose (50 percent kill): 1225 mg/kg | GISAAA 53(6),78,1988 | |
| unreported route/rat | lethal dose (50 percent kill): 5350 mg/kg | GISAAA 53(6),78,1988 |
Other Multiple Dose Data and References
| Route/Organism | Dose | Effect | Reference |
|---|---|---|---|
| inhalation/Dog | lowest published toxic concentration: 1000 ppm/24H/7W- continuous | Behavioral: Food intake (animal) Musculoskeletal: Other changes Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field | NTIS** AD746-295 |
| inhalation/Dog | lowest published toxic concentration: 5000 ppm/6H/12W- intermittent | Behavioral: Hallucinations, distorted perceptions Behavioral: Ataxia Behavioral: Antipsychotic | NTIS** OTS0555748 |
| inhalation/Dog | lowest published toxic concentration: 34 gm/m3/7W- intermittent | Behavioral: General anesthetic Liver: Fatty liver degeneration | TPKVAL 1,96,1961 |
| inhalation/guinea pig | lowest published toxic concentration: 34 gm/m3/7W- intermittent | Behavioral: General anesthetic Liver: Fatty liver degeneration | TPKVAL 1,96,1961 |
| inhalation/guinea pig | lowest published toxic concentration: 552 ppm/6H/5D- intermittent | Liver: Other changes Biochemical: Metabolism (intermediary): Lipids including transport | AIHAAP 37,345,1976 |
| inhalation/monkey | lowest published toxic concentration: 5000 ppm/24H/32D- continuous | Behavioral: Food intake (animal) Nutritional and Gross Metabolic: Weight loss or decreased weight gain Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field | NTIS** AD746-295 |
| inhalation/mouse | lowest published toxic concentration: 13000 ppm/6H/19D- intermittent | Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field | NTPTR* NTP-TR-306,1986 |
| inhalation/mouse | lowest published toxic concentration: 150 ppm/24H/30D- continuous | Liver: Changes in liver weight Endocrine: Changes in spleen weight Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other esterases | APTOA6 59,73,1986 |
| inhalation/mouse | lowest published toxic concentration: 5000 ppm/24H/7D- continuous | Liver: Changes in liver weight Nutritional and Gross Metabolic: Weight loss or decreased weight gain | TXAPA9 23,660,1972 |
| inhalation/mouse | lowest published toxic concentration: 300 ppm/24H/90D- continuous | Liver: Changes in liver weight Endocrine: Changes in spleen weight Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other esterases | APTOA6 59,73,1986 |
| inhalation/mouse | lowest published toxic concentration: 1.04 gm/m3/5D- intermittent | Lung, Thorax, or Respiration: Other changes Liver: Other changes Kidney, Ureter, and Bladder: Other changes | TOXID9 66,62,2002 |
| inhalation/mouse | lowest published toxic concentration: 49567.7 mg/kg/13W- intermittent | Liver: Other changes | MUREAV 746,135,2012 |
| inhalation/rat | lowest published toxic concentration: 0.045 gm/m3/91D- intermittent | Behavioral: Alteration of classical conditioning | TPKVAL 1,96,1961 |
| inhalation/rat | lowest published toxic concentration: 0.25 gm/m3/24D- intermittent | Behavioral: Alteration of classical conditioning | TPKVAL 1,96,1961 |
| inhalation/rat | lowest published toxic concentration: 5000 ppm/28D- intermittent | Liver: Changes in liver weight Endocrine: Differential effect of sex or castration on observed toxicity Endocrine: Changes in spleen weight | JTEHF8 66,1207,2003 |
| inhalation/rat | lowest published toxic concentration: 2800 mg/m3/4D- intermittent | Peripheral Nerve and Sensation: Recording from peripheral motor nerve Liver: Liver function tests impaired Kidney, Ureter, and Bladder: Other changes | TPKVAL 15,76,1979 |
| inhalation/rat | lowest published toxic concentration: 2800 mg/m3/8D- intermittent | Kidney, Ureter, and Bladder: Other changes in urine composition | TPKVAL 15,76,1979 |
| inhalation/rat | lowest published toxic concentration: 2800 mg/m3/27D- intermittent | Nutritional and Gross Metabolic: Weight loss or decreased weight gain | TPKVAL 15,76,1979 |
| inhalation/rat | lowest published toxic concentration: 880 mg/m3/4D- intermittent | Peripheral Nerve and Sensation: Recording from peripheral motor nerve | TPKVAL 15,76,1979 |
| inhalation/rat | lowest published toxic concentration: 880 mg/m3/15D- intermittent | Kidney, Ureter, and Bladder: Other changes in urine composition | TPKVAL 15,76,1979 |
| inhalation/rat | lowest published toxic concentration: 0.4 gm/m3/91D- intermittent | Brain and Coverings: Other degenerative changes | TPKVAL 1,72,1961 |
| inhalation/rat | lowest published toxic concentration: 3700 ppm/5H/4W- intermittent | Lung, Thorax, or Respiration: Other changes Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Phosphatases Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Dehydrogenases | TOLED5 7,41,1980 |
| inhalation/rat | lowest published toxic concentration: 5000 ppm/24H/14W- continuous | Liver: Other changes Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Changes in bladder weight | NTIS** AD746-295 |
| inhalation/rat | lowest published toxic concentration: 8400 ppm/6H/13W- intermittent | Liver: Changes in liver weight Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field | NTPTR* NTP-TR-306,1986 |
| inhalation/rat | lowest published toxic concentration: 500 ppm/6H/2W- intermittent | Kidney, Ureter, and Bladder: Other changes Blood: Methemoglobinemia- Carboxhemoglobinemia Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other enzymes | RCOCB8 32,535,1981 |
| inhalation/rat | lowest published toxic concentration: 1000 ppm/2H/3W- intermittent | Behavioral: Food intake (animal) Liver: Other changes Blood: Other changes | HOCHE8 8,64,1990 |
| inhalation/rat | lowest published toxic concentration: 1000 ppm/6H/3D- intermittent | Brain and Coverings: Other degenerative changes Biochemical: Neurotransmitters or modulators (putative): Dopamine at other sites | TXCYAC 29,293,1984 |
| oral/mouse | lowest published toxic dose: 1862 mg/kg/2W- intermittent | Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) | DCTODJ 6,563,1983 |
| oral/mouse | lowest published toxic dose: 52740 mg/kg/90D- continuous | Liver: Hepatitis (hepatocellular necrosis), zonal Liver: Fatty liver degeneration | FCTOD7 24,943,1986 |
| oral/rat | lowest published toxic dose: 14940 mg/kg/90D- continuous | Liver: Hepatitis (hepatocellular necrosis), zonal Liver: Fatty liver degeneration | FCTOD7 24,943,1986 |
| oral/rat | lowest published toxic dose: 91 gm/kg/2Y- continuous | Behavioral: Fluid intake Liver: Fatty liver degeneration Nutritional and Gross Metabolic: Weight loss or decreased weight gain | FCTOD7 24,951,1986 |
| skin/rat | lowest published toxic dose: 39270 mg/kg/17W- intermittent | Liver: Other changes Liver: Changes in liver weight | GTPZAB 26(5),41,1982 |
Reviews
| Organization | Standard | Reference |
|---|---|---|
| American Conference of Governmental Industrial Hygienists (ACGIH) | Threshold Limit Value-time-weighted average 50 ppm | DTLVS* TLV/BEI,2013 |
| American Conference of Governmental Industrial Hygienists (ACGIH) | Threshold Limit Value-Confirmed animal carcinogen | DTLVS* TLV/BEI,2013 |
| International Agency for Research on Cancer (IARC) | Cancer Review:Animal Sufficient Evidence | IMEMDT 41,43,1986 |
| International Agency for Research on Cancer (IARC) | Cancer Review:Animal Sufficient Evidence | IMEMDT 71,251,1999 |
| International Agency for Research on Cancer (IARC) | Cancer Review:Human Inadequate Evidence | IMEMDT 41,43,1986 |
| International Agency for Research on Cancer (IARC) | Cancer Review:Human Inadequate Evidence | IMEMDT 71,251,1999 |
| International Agency for Research on Cancer (IARC) | Cancer Review:Group 2B | IMEMDT 71,251,1999 |
| TOXICOLOGY REVIEW | FAZMAE 18,365,1974 | |
| TOXICOLOGY REVIEW | TOLED5 127,135,2002 | |
| TOXICOLOGY REVIEW | TOLED5 138,9,2003 | |
| TOXICOLOGY REVIEW | MUREAV 543,201,2003 | |
| TOXICOLOGY REVIEW | MUREAV 567,227,2004 | |
| TOXICOLOGY REVIEW | MUREAV 567,109,2004 | |
| TOXICOLOGY REVIEW | MUREAV 567,151,2004 | |
| TOXICOLOGY REVIEW | MUREAV 567,347,2004 | |
| TOXICOLOGY REVIEW | EMMUEG 39,69,2002 | |
| TOXICOLOGY REVIEW | DIMON* 46,551,2000 | |
| TOXICOLOGY REVIEW | DIMON* 39,678,1993 | |
| TOXICOLOGY REVIEW | MUREAV 628,67,2007 | |
| TOXICOLOGY REVIEW | ENTOX* -,92,2005 | |
| TOXICOLOGY REVIEW | MUREAV 584,1,2005 | |
| TOXICOLOGY REVIEW | BCLPT* 96,131,2005 | |
| TOXICOLOGY REVIEW | MUREAV 636,242,2007 | |
| TOXICOLOGY REVIEW | MUREAV 654,114,2008 | |
| TOXICOLOGY REVIEW | HUTOX* -,661,1996 | |
| TOXICOLOGY REVIEW | HUTOX* -,683,1996 | |
| TOXICOLOGY REVIEW | HUTOX* -,577,1996 | |
| TOXICOLOGY REVIEW | HTOPA* 1,501,2002 | |
| TOXICOLOGY REVIEW | TOLED5 180,100,2008 | |
| TOXICOLOGY REVIEW | AIRMJ* 20,8,2001 | |
| TOXICOLOGY REVIEW | MUREAV 682,39,2009 | |
| TOXICOLOGY REVIEW | NCLNA* 16,503,1998 | |
| TOXICOLOGY REVIEW | NTAPM* -,629,1995 | |
| TOXICOLOGY REVIEW | NTAPM* -,711,1995 | |
| TOXICOLOGY REVIEW | NTAPM* -,727,1995 | |
| TOXICOLOGY REVIEW | MUREAV 705,184,2010 | |
| TOXICOLOGY REVIEW | MUREAV 751,49,2012 | |
| TOXICOLOGY REVIEW | MUREAV 752,99,2013 | |
| TOXICOLOGY REVIEW | TXAPA9 254,100,2011 | |
| TOXICOLOGY REVIEW | TXAPA9 255,113,2011 | |
| TOXICOLOGY REVIEW | MUREAV 769,34,2014 | |
| TOXICOLOGY REVIEW | MUREAV 762,76,2014 | |
| TOXICOLOGY REVIEW | FCTOD7 55,596,2013 | |
| TOXICOLOGY REVIEW | TXAPA9 266,345,2013 | |
| TOXICOLOGY REVIEW | MUREAV 802,1,2016 | |
| TOXICOLOGY REVIEW | MUREAV 770,92,2016 | |
| TOXICOLOGY REVIEW | MUREAV 770,170,2016 | |
| TOXICOLOGY REVIEW | REPTED 71,71,2017 | |
| TOXICOLOGY REVIEW | RTOPDW 58,395,2010 | |
| TOXICOLOGY REVIEW | RTOPDW 59,364,2011 |
Standards and Regulations
| Organization | Standard | Reference |
|---|---|---|
| Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION | FEREAC 54,7740,1989 | |
| Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1998 STATUS OF PESTICIDES | Cancelled | RBREV* -,263,1998 |
| Mine Safety and Health Administration (MSHA) STANDARD-air | time-weighted average 500 ppm (1750 mg/m3) | DTLVS* 3,171,1971 |
| Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV; | ||
| Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV | ||
| Occupational Exposure Limit-AUSTRALIA | time-weighted average 50 ppm (174 mg/m3), Carcinogen, JUL2008 | |
| Occupational Exposure Limit-AUSTRIA | MAK-TMW 50 ppm (175 mg/m3);KZW 200 ppm (700 mg/m3), skin, 2007 | |
| Occupational Exposure Limit-BELGIUM | time-weighted average 50 ppm (177 mg/m3), MAR2002 | |
| Occupational Exposure Limit-DENMARK | time-weighted average 35 ppm (122 mg/m3), skin, carc, MAY2011 | |
| Occupational Exposure Limit-FINLAND | time-weighted average 100 ppm (350 mg/m3), short term exposure limit 250 ppm (880 mg/m3), NOV2011 | |
| Occupational Exposure Limit-FRANCE | VME 50 ppm (180 mg/m3), VLE 100 ppm, C3 Carcinogen, FEB2006 | |
| Occupational Exposure Limit-HUNGARY | time-weighted average 10 mg/m3, short term exposure limit 10 mg/m3, SEP2000 | |
| Occupational Exposure Limit-ICELAND | time-weighted average 35 ppm (122 mg/m3), skin, NOV2011 | |
| Occupational Exposure Limit-JAPAN | Occupational Exposure Limit 50 ppm (170 mg/m3), Occupational Exposure Limit-continuous 100 ppm (340 mg/m3), skin, 2B carc, MAY2012 | |
| Occupational Exposure Limit-KOREA | time-weighted average 50 ppm (175 mg/m3), 2006 | |
| Occupational Exposure Limit-MEXICO | time-weighted average 100 ppm (330 mg/m3);short term exposure limit 500 ppm (1740 mg/m3), 2004 | |
| Occupational Exposure Limit-NEW ZEALAND | time-weighted average 50 ppm (174 mg/m3), JAN2002 | |
| Occupational Exposure Limit-NORWAY | time-weighted average 35 ppm (125 mg/m3), JAN1999 | |
| Occupational Exposure Limit-PERU | time-weighted average 50 ppm (174 mg/m3), JUL2005 | |
| Occupational Exposure Limit-POLAND | MAC(time-weighted average) 20 mg/m3, short term exposure limit 50 mg/m3, JAN1999 | |
| Occupational Exposure Limit-RUSSIA | time-weighted average 50 mg/m3, short term exposure limit 100 mg/m3, JUN2003 | |
| Occupational Exposure Limit-SWEDEN | time-weighted average 35 ppm (120 mg/m3);short term exposure limit 70 ppm (250 mg/m3), Skin, Carcinogen, JUN2005 | |
| Occupational Exposure Limit-SWITZERLAND | MAK-week 50 ppm (180 mg/m3), carc 3, JAN2011 | |
| Occupational Exposure Limit-THAILAND | time-weighted average 500 mg/m3, short term exposure limit 1000 mg/m3, JAN1993 | |
| Occupational Exposure Limit-THE NETHERLANDS | MAC-TGG 350 mg/m3, 2003 | |
| Occupational Exposure Limit-THE PHILIPPINES | time-weighted average 500 ppm (1740 mg/m3), JAN1993 | |
| Occupational Exposure Limit-TURKEY | time-weighted average 500 ppm (1740 mg/m3), JAN1993 | |
| Occupational Exposure Limit-UNITED KINGDOM | time-weighted average 100 ppm (350 mg/m3);short term exposure limit 300 ppm (1060 mg/m3), skin, OCT2007 | |
| Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction) | see 56 FR 57036 | CFRGBR 29,1926.55,1994 |
| Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors) | 8H time-weighted average 500 ppm (1740 mg/m3) | CFRGBR 41,50-204.50,1994 |
| Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry) | 8H time-weighted average 500 ppm;ceiling concentration 1000 ppm;Pk 2000 ppm/5M/2H | CFRGBR 29,1910.1000,1994 |
| Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards) | 8H time-weighted average 500 ppm (1740 mg/m3 | CFRGBR 29,1915.1000,1993 |
NIOSH Documentation and Surveillance
| Organization | Standard | Reference |
|---|---|---|
| National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO METHYLENE CHLORIDE-air | Carcinogen lowest feasible concentration | NIOSH* DHHS #92-100,1992 |
| National Occupational Exposure Survey 1983 | Hazard Code 47270; Number of Industries 363; Total Number of Facilities 87086; Number of Occupations 212; Total Number of Employees Exposed 1438196; Total Number of Female Employees Exposed 352536 | |
| National Occupational Hazard Survey 1974 | Hazard Code 47270; Number of Industries 374; Total Number of Facilities 89025; Number of Occupations 192; Total Number of Employees Exposed 975696 |
Status in Federal Agencies
| Organization | Reference |
|---|---|
| ATSDR TOXICOLOGY PROFILE (NTIS** PB/2000/108026) | |
| EPA GENETOX PROGRAM 1988, Negative: D melanogaster Sex-linked lethal | |
| EPA GENETOX PROGRAM 1988, Positive: Cell transform.-RLV F344 rat embryo | |
| EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test | |
| EPA GENETOX PROGRAM 1988, Positive: S cerevisiae gene conversion; S cerevisiae-homozygosis | |
| EPA GENETOX PROGRAM 1988, Positive: S cerevisiae-reversion | |
| EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULE | FEREAC 47,26992,82 |
| EPA TSCA Section 8(b) CHEMICAL INVENTORY | |
| EPA TSCA Section 8(d) unpublished health/safety studies | |
| EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001 | |
| NCI Carcinogenesis Studies (inhal);clear evidence:mouse,rat | |
| NIOSH Analytical Method, 1994: Methylene chloride, 1005 | |
| NIOSH Analytical Method, 1996: Volatile organic compound, 2549 | |
| NIOSH CURRENT INTELLIGENCE BULLETIN 46, 1986 | |
| NTP 14th Report on Carcinogens,2016:Reasonably anticipated to be human carcinogen | |
| On EPA IRIS database | |
| OSHA ANALYTICAL METHOD #80 |
Page last reviewed: November 16, 2018
Content
source: National Institute for Occupational Safety and Health