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Benzene, m - dinitro -

RTECS #: CZ7350000

CAS #: 99-65-0


UPDATE: May 2009 MW: 168.12 MF: C6H4N2O4


NOTE:

TABLE OF CONTENTS:
  1. SYNONYMS:
  2. SKIN AND EYE IRRITATION DATA:
  3. MUTATION DATA:
  4. REPRODUCTIVE EFFECTS DATA:
  5. ACUTE TOXICITY DATA:
  6. OTHER MULTIPLE DOSE DATA:
  7. REVIEWS:
  8. STANDARDS AND REGULATIONS:
  9. NIOSH DOCUMENTATION AND SURVEILLANCE:
  10. STATUS IN FEDERAL AGENCIES:
  11. REFERENCES:

SYNONYMS:

  1. 1,3 - Dinitrobenzene
  2. 1,3 - Dinitrobenzol
  3. 2,4 - Dinitrobenzene
  4. Benzene, 1,3 - dinitro -
  1. Binitrobenzene
  2. Dwunitrobenzen (Polish)
  3. m - Dinitrobenzene
  4. m - Dinitrobenzene (ACGIH:OSHA)


SKIN AND EYE IRRITATION DATA AND REFERENCES:

ROUTE/
ORGANISM
DOSE
EFFECT

REFERENCE
eye
rabbit
100 mg N/R ATDAEI 1,168,1992


MUTATION DATA AND REFERENCES:

SYSTEM TEST ROUTE/
ORGANISM/
TISSUE
DOSE REFERENCE
DNA damage rat testis 0.4 mol/L EMMUEG 44,179,2004
mutation in microorganisms Salmonella typhimurium 3,300 ng/plate (+enzymatic activation step) ENMUDM 2,531,1980
mutation in microorganisms Salmonella typhimurium 50 g/plate (-enzymatic activation step) MUREAV 44,9,1977
other mutation test systems Salmonella typhimurium 12,500 g/L MUREAV 272,91,1992


REPRODUCTIVE EFFECTS DATA AND REFERENCES:

ROUTE/
ORGANISM
DOSE
EFFECT

REFERENCE
intraperitoneal
rat
lowest published toxic dose: 10 g/kg (1 day male) Reproductive: Other effects on male REPTED 14,45,2000
oral
mouse
lowest published toxic dose: 48 mg/kg (1 day male) Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct
JTEHD6 28,67,1989
oral
rat
lowest published toxic dose: 24 mg/kg (1 day male) Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct
FAATDF 14,284,1990
oral
rat
lowest published toxic dose: 33,600 g/kg (16 week prior to copulation) Reproductive: Maternal effects: Ovaries, fallopian tubes JTEHD6 7,829,1981
oral
rat
lowest published toxic dose: 48 mg/kg (1 day male) Reproductive: Effects on fertility: Male fertility index (e.g., # males impregnating females per # males exposed to fertile nonpregnant females) FAATDF 14,284,1990
oral
rat
lowest published toxic dose: 90 mg/kg (12 week male) Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) JTEHD6 19,477,1986


TUMORIGENIC DATA AND REFERENCES:

ROUTE/
ORGANISM
DOSE
EFFECT

REFERENCE
N/R
N/R N/R N/R


ACUTE TOXICITY DATA AND REFERENCES:

ROUTE/
ORGANISM
DOSE
EFFECT

REFERENCE
intraperitoneal
mouse
lowest published lethal dose: 200 mg/kg N/R JAPMA8 48,419,1959
intraperitoneal
rat
lethal dose (50 percent kill): 28 mg/kg N/R AEPPAE 207,446,1949
intravenous
dog
lethal dose (50 percent kill): 10 mg/kg Peripheral Nerve and Sensation: Spastic paralysis with/without sensory change

Behavioral: Convulsions or effect on seizure threshold

Blood: Methemoglobinemia- Carboxhemoglobinemia
NTIS** AD-A066-307
oral
wild bird
lethal dose (50 percent kill): 42 mg/kg N/R TXAPA9 21,315,1972
oral
cat
lowest published lethal dose: 27 mg/kg Behavioral: Coma

Lung, Thorax, or Respiration: Cyanosis

Blood: Other hemolysis with or without anemia
LANCAO 2,582,1901
oral
dog
lowest published lethal dose: 600 mg/kg N/R NTIS** AD-A066-307
oral
human
lowest published lethal dose: 28 mg/kg N/R 34ZIAG -,226,1969
oral
mouse
lethal dose (50 percent kill): 74,700 g/kg Behavioral: Somnolence (general depressed activity)

Behavioral: Tremor

Lung, Thorax, or Respiration: Respiratory stimulation
ATDAEI 1,168,1992
oral
rat
lethal dose (50 percent kill): 59,500 g/kg Behavioral: Somnolence (general depressed activity)

Lung, Thorax, or Respiration: Dyspnea

Skin and Appendages: Other: Hair
ATDAEI 1,168,1992
oral
rat
lowest published toxic dose: 15 mg/kg Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct
TXAPA9 92,54,1988
oral
rat
lowest published toxic dose: 50 mg/kg Blood: Changes in spleen

Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Related to Chronic Data: Changes in testicular weight
TXAPA9 92,54,1988
oral
rat
lowest published toxic dose: 30 mg/kg Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Related to Chronic Data: Changes in testicular weight
ARTODN 66,435,1992
oral
rat
lowest published toxic dose: 60 mg/kg Kidney, Ureter, and Bladder: Other changes in urine composition

Reproductive: Paternal effects: Testes, epididymis, sperm duct
ARTODN 66,435,1992
oral
rat
lowest published toxic dose: 32 mg/kg Endocrine: Androgenic

Reproductive: Paternal effects: Testes, epididymis, sperm duct

Related to Chronic Data: Changes in testicular weight
TXAPA9 95,255,1988
oral
rabbit
lowest published lethal dose: 400 mg/kg N/R NTIS** AD-A066-307
skin
man
lowest published toxic dose: 4 mg/kg/2 day- intermittent Behavioral: Change in motor activity (specific assay)

Lung, Thorax, or Respiration: Cyanosis
LANCAO 2,582,1901
skin
rabbit
lethal dose (50 percent kill): 1,900 mg/kg N/R ATDAEI 1,168,1992


OTHER MULTIPLE DOSE DATA AND REFERENCES:

ROUTE/
ORGANISM
DOSE
EFFECT

REFERENCE
implant
rat
lowest published toxic dose: 30 mg/kg/6 day- continuous Brain and Coverings: Other degenerative changes

Blood: Hemorrhage
TXAPA9 161,267,1999
intraperitoneal
rat
lowest published toxic dose: 30 mg/kg/4 day- intermittent Brain and Coverings: Other degenerative changes

Behavioral: Ataxia

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NRTXDN 20,83,1999
intraperitoneal
rat
lowest published toxic dose: 30 mg/kg/2 day- intermittent Brain and Coverings: Other degenerative changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other oxidoreductases

Biochemical: Metabolism (intermediary): Other
TOXID9 44,380,2005
oral
rat
lowest published toxic dose: 156 mg/kg/90 day- intermittent Endocrine: Changes in spleen weight

Blood: Methemoglobinemia- Carboxhemoglobinemia
TOXID9 30,200,1996
oral
rat
lowest published toxic dose: 265 mg/kg/8 week- continuous Blood: Pigmented or nucleated red blood cells

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Changes in testicular weight
JTEHD6 7,829,1981
oral
rat
lowest published toxic dose: 296 mg/kg/16 week- continuous Endocrine: Changes in spleen weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Changes in testicular weight
JTEHD6 7,829,1981
oral
rat
lowest published toxic dose: 700 mg/kg/2 week- intermittent Reproductive: Paternal effects: Testes, epididymis, sperm duct

Related to Chronic Data: Changes in testicular weight
JTSCDR 25,251,2000
unreported route
rat
lowest published toxic dose: 9 mg/kg/3 day- intermittent Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Paternal effects: Testes, epididymis, sperm duct

Biochemical: Metabolism (intermediary): Other
TOXID9 90,219,2006


REVIEWS:

ORGANIZATION STANDARD
REFERENCE

American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value time-weighted average 0.15 ppm (skin) DTLVS* TLV/BEI,2007
TOXICOLOGY REVIEW
JTSCDR 25,1,2000
TOXICOLOGY REVIEW
TOLED5 140-141,105,2003
TOXICOLOGY REVIEW
MUREAV 567,347,2004
TOXICOLOGY REVIEW
REPTED 26,185,2008


STANDARDS AND REGULATIONS:

ORGANIZATION STANDARD
REFERENCE

Mine Safety and Health Administration (MSHA) STANDARD - air time-weighted average 0.15 ppm (1 mg/m3) (skin) DTLVS* 3,92,1971
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry) 8 hour time-weighted average 1 mg/m3 (skin) CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction) 8 hour time-weighted average 1 mg/m3 (skin) CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards) 8 hour time-weighted average 1 mg/m3 (skin) CFRGBR 29,1915.1000,1993
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors) 8 hour time-weighted average 1 mg/m3 (skin) CFRGBR 41,50-204.50,1994
Occupational Exposure Limit - AUSTRALIA time-weighted average 0.15 ppm (1 mg/m3), JUL2008
Occupational Exposure Limit - FRANCE VME 0.15 ppm (1 mg/m3), FEB2006
Occupational Exposure Limit - HUNGARY time-weighted average 1 mg/m3, short term exposure limit 2 mg/m3, Skin, SEP2000
Occupational Exposure Limit - JAPAN Occupational Exposure Limit 0.15 ppm (1 mg/m3), skin, APR2007
Occupational Exposure Limit - KOREA time-weighted average 0.15 ppm (1 mg/m3), skin, 2006
Occupational Exposure Limit - THE NETHERLANDS MAC-TGG 1 mg/m3, 2003
Occupational Exposure Limit - NEW ZEALAND time-weighted average 0.15 ppm (1 mg/m3), skin, JAN2002
Occupational Exposure Limit - SWEDEN time-weighted average 0.15 ppm (1 mg/m3);
short term exposure limit 0.3 ppm (2 mg/m3), Skin, JUN2005
Occupational Exposure Limit - SWITZERLAND MAK- week 0.15 ppm (1 mg/m3),KZG- week 0.3 ppm (2 mg/m3), Skin, DEC2006


NIOSH DOCUMENTATION AND SURVEILLANCE:

ORGANIZATION STANDARD or SURVEY
REFERENCE

National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO m-DINITROBENZENE-air 10 hour time-weighted average 1 mg/m3 (Sk) NIOSH* DHHS #92-100,1992
National Occupational Hazard Survey 1974 National Occupational Hazard Survey 1974: Hazard Code: 27046;
Number of Industries 2;
Total Number of Facilities 184;
Number of Occupations 3;
Total Number of Employees Exposed 1,754
National Occupational Exposure Survey 1983 National Occupational Exposure Survey 1983: Hazard Code: 27046;
Number of Industries 2;
Total Number of Facilities 41;
Number of Occupations 4;
Total Number of Employees Exposed 2,489;
Total Number of Female Employees Exposed 1,914


STATUS IN FEDERAL AGENCIES:

ORGANIZATION
REFERENCE

EPA GENETOX PROGRAM 1988, Positive: Histidine reversion-Ames test
EPA TSCA Section 8(b) CHEMICAL INVENTORY
On EPA IRIS database
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001


REFERENCES:

CODEN
REFERENCE

34ZIAG "Toxicology of Drugs and Chemicals," Deichmann, W.B., New York, Academic Press, Inc., 1969
AEPPAE Naunyn-Schmiedeberg's Archiv fuer Experimentelle Pathologie und Pharmakologie. (Berlin, Ger.) V.110-253, 1925-66. For publisher information, see NSAPCC.
ARTODN Archives of Toxicology. (Springer-Verlag, Heidelberger Pl. 3, D-1000 Berlin 33, Fed. Rep. Ger.) V.32- 1974-
ATDAEI Acute Toxicity Data. Journal of the American College of Toxicology, Part B. (Mary Ann Liebert, Inc., 1651 Third Ave., New York, NY 10128) V.1- 1990-
CFRGBR Code of Federal Regulations. (U.S. Government Printing Office, Supt. of Documents, Washington, DC 20402)
DTLVS* The Threshold Limit Values (TLVs) and Biological Exposure Indices (BEIs) booklet issues by American Conference of Governmental Industrial Hygienists (ACGIH), Cincinnati, OH, 1996
EMMUEG Environmental and Molecular Mutagenesis. (Alan R. Liss, Inc., 41 E. 11th St., New York, NY 10003) V.10- 1987-
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG.
FAATDF Fundamental and Applied Toxicology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1-40, 1981-97. For publisher information, see TOSCF2
JAPMA8 Journal of the American Pharmaceutical Association, Scientific Edition. (Washington, DC) V.29-49, 1940-60. For publisher information, see JPMSAE.
JTEHD6 Journal of Toxicology and Environmental Health. (Hemisphere Pub., 1025 Vermont Ave., NW, Washington, DC 20005) V.1- 1975/76-
JTSCDR Journal of Toxicological Sciences. (Japanese Soc. of Toxicological Sciences, 4th Floor, Gakkai Center Bldg., 4-16, Yayoi 2-chome, Bunkyo-ku, Tokyo 113, Japan) V.1- 1976-
LANCAO Lancet. (7 Adam St., London WC2N 6AD, UK) V.1- 1823-
MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964-
NIOSH* National Institute of Occupational Safety and Health, U.S. Dept. of Health, Education, and Welfare, Reports and Memoranda.
NRTXDN Neurotoxicology. (Intox Press, Inc., POB 34075, Little Rock, AR 72203) V.1- 1979-
NTIS** National Technical Information Service. (Springfield, VA 22161) Formerly U.S. Clearinghouse for Scientific & Technical Information.
REPTED Reproductive Toxicology. (Pergamon Press Inc., Maxwell House, Fairview Park, Elmsford, NY 10523) V.1- 1987-
TOLED5 Toxicology Letters. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1977-
TOXID9 Toxicologist. (Soc. of Toxicology, Inc., 475 Wolf Ledge Parkway, Akron, OH 44311) V.1- 1981-
TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959-

NIOSH PROFILE (NITROBENZENES), SRC, 10/77

RTECS Compound Description:
   Mutagen
   Reproductive Effector
   Human Data
   Primary Irritant

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