Table of Contents

Locator Record

Title: IgG and IgM Antibody to Hepatitis E Virus (SSNH3HEG )
Contact Number: 1-866-441-NCHS
Years of Content: 1988 - 1994
First Published: April, 2013
Revised: May, 2013
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Subject:IgG and IgM Antibody to Hepatitis E Virus
Record Source: NHANES 1988 - 1994
Survey Methodology: NHANES 1988 - 1994 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

IgG and IgM hepatitis E virus (HEV) antibody testing of stored sera specimens from NHANES III was conducted to estimate the seroprevalence of HEV infection (anti-HEV IgG and IgM) in participants ≥ 6 years of age.

Eligible Sample

A stratified random sample of participants ≥6 years of age from NHANES III with anti-HEV IgG test results from testing performed by Kuniholm et al. was selected for testing by the CDC Hepatitis Laboratory.

Description of Laboratory Methodology

Anti-HEV (IgG)
DS-EIA-ANTI-HEV-G is an enzyme immunoassay kit intended for the detection of IgG antibodies to hepatitis E virus in human serum or plasma. During an initial incubation, HEV antibody in the sample binds with HEV antigen coated onto wells of a polystyrene stripped plate. Unbound sample is removed by washing. During a second incubation, horseradish peroxidase-labeled antibody conjugate (monoclonal mouse antibodies against human IgG) binds to any human IgG from the sample that was captured on the well. Unbound conjugate is removed by washing and a substrate solution containing tetramethylbenzidine is added to produce color. The reaction is stopped by adding a sulphuric acid solution and the OD of each well is read. The presence or absence of IgG antibodies to hepatitis E virus is determined by the ratio of the OD of each sample to the calculated cut-off value. A sample is considered negative if the sample OD value is < cut-off and is considered positive if the sample OD value is ≥ cut-off.

Anti-HEV (IgM)
DS-EIA-ANTI-HEV-M is an enzyme immunoassay kit intended for the detection of IgM antibodies to hepatitis E virus in human serum or plasma. During an initial incubation, HEV antibody in the sample binds with HEV antigen coated onto wells of a polystyrene stripped plate. Unbound sample is removed by washing. During a second incubation, horseradish peroxidase-labeled antibody conjugate (monoclonal mouse antibodies against human IgM) binds to any human IgM from the sample that was captured on the well. Unbound conjugate is removed by washing and a substrate solution containing tetramethylbenzidine is added to produce color. The reaction is stopped by adding a sulphuric acid solution and the OD of each well is read.The presence or absence of IgM antibodies to hepatitis E virus is determined by the ratio of the OD of each sample to the calculated cut-off value. A sample is considered negative if the sample OD value is < cut-off and is considered positive if the sample OD value is ≥ cut-off.

Data Processing and Editing

Data was received after all the laboratory testing was complete. The data were not edited.

Data Access: All data are publicly available.

Laboratory Quality Assurance and Monitoring

The NHANES quality assurance and quality control (QA/QC) protocols meet the 1988 Clinical Laboratory Improvement Act mandates.

Analytic Notes

There are three variables:
SEQN: Respondent sequence number
SSHEG: Anti-HEV IgG (1=Positive, 2=Negative)
SSHEM: Anti-HEV IgM (1=Positive, 2=Negative)

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 6 YEARS - 150 YEARS

SSHEG - Anti-HEV IgG

Variable Name:
SSHEG
SAS Label:
Anti-HEV IgG
English Text:
Anti-HEV IgG
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 1000 1000
2 Negative 4966 5966
. Missing 7 5973

SSHEM - Anti-HEV IgM

Variable Name:
SSHEM
SAS Label:
Anti-HEV IgM
English Text:
Anti-HEV IgM
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 140 140
2 Negative 5828 5968
. Missing 5 5973

SSWEIGHT - Sampling weight for retest subsample

Variable Name:
SSWEIGHT
SAS Label:
Sampling weight for retest subsample
English Text:
Sampling weight for retest subsample
Target:
Both males and females 6 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1.9395 to 3.65125 Range of Values 5973 5973
. Missing 0 5973