Table of Contents

Locator Record

Title: Polyfluorinated Compounds (PFC_F )
Contact Number: 1-866-441-NCHS
Years of Content: 2009 - 2010
First Published: December, 2011
Revised: October, 2013
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Subject:Polyfluorinated Compounds
Record Source: NHANES 2009 - 2010
Survey Methodology: NHANES 2009 - 2010 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

Organic fluorochemicals are used in multiple commercial applications including surfactants, lubricants, paints, polishes, food packaging and fire-retarding foams. Several of these polyfluorochemicals (PFCs) are ubiquitous contaminants found both in humans and animals worldwide. Perfluorooctane sulfonate (PFOS) has been used in a wide variety of industrial and consumer products including protective coatings for carpets and apparel, paper coatings, insecticide formulations, and surfactants. In May 2000, the 3M Company, the sole manufacturer of PFOS in the United States and the principal manufacturer worldwide, announced that it was discontinuing the production of perfluorooctanyl fluoride-based chemistries, including PFOS. Perfluorooctanoic acid (PFOA) is used primarily to produce its salts which are used in the production of fluoroelastomers and fluoropolymers, such as polytetrafluoroethylene (PTFE) and polyvinylidine fluoride (PVDF). PTFE has numerous uses in many industrial and consumer products, including coatings on textiles and carpet; uses in the automotive, mechanical, aerospace, chemical, electrical, medical, and building/construction industries; personal care products; and non-stick coatings on cookware. PVDF is used primarily in electrical/electronics, building/construction, and chemical processing industrial sectors.

Eligible Sample

Participants aged 12 years and older who met the subsample requirements.

Description of Laboratory Methodology

Solid phase extraction coupled to High Performance Liquid Chromatography-Turbo Ion Spray ionization-tandem Mass Spectrometry (online SPE-HPLC-TIS-MS/MS) is used for the quantitative detection of perfluorooctane sulfonamide (PFOSA), 2-(N-methyl-perfluorooctane sulfonamido) acetic acid (Me-PFOSA-AcOH), 2-(N-ethyl-perfluorooctane sulfonamido) acetic acid (Et-PFOSA-AcOH), perfluorobutane sulfonate (PFBuS), perfluorohexane sulfonate (PFHxS), perfluorooctane sulfonate (PFOS), perfluoroheptanoate (PFHpA), perfluorooctanoate (PFOA), perfluorononanoate (PFNA), perfluorodecanoate (PFDeA), perfluoroundecanoate (PFUA), and perfluorododecanoate (PFDoA) (Kuklenyik Z, et al. 2005). Briefly, after dilution with formic acid, one aliquot of 100 μL of serum is injected into a commercial column switching system allowing for concentration and chromatographic separation of the analytes. Detection and quantification are done using tandem mass spectrometry (Kuklenyik Z, et al. 2005).

Laboratory Quality Assurance and Monitoring

Serum specimens are processed, stored, and shipped to the Division of Environmental Health Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention for analysis.

Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Vials are stored under appropriate frozen (–20°C) conditions until they are shipped to National Center for Environmental Health for testing.

Mobile Examination Centers (MECs)
Laboratory team performance is monitored using several techniques. NCHS and contract consultants use a structured quality assurance evaluation during unscheduled visits to evaluate both the quality of the laboratory work and the quality-control procedures. Each laboratory staff person is observed for equipment operation, specimen collection and preparation; testing procedures and constructive feedback are given to each staff. Formal retraining sessions are conducted annually to ensure that required skill levels were maintained.

The NHANES QA/QC protocols meet the 1988 Clinical Laboratory Improvement Act mandates. Detailed QA/QC instructions are discussed in the NHANES LPM.

Analytical Laboratories
NHANES uses several methods to monitor the quality of the analyses performed by the contract laboratories. In the MEC, these methods include performing blind split samples collected on “dry run” sessions. In addition, contract laboratories randomly perform repeat testing on 2.0% of all specimens.

NCHS developed and distributed a quality control protocol for all the contract laboratories which outlined the Westgard rules used when running NHANES specimens. Progress reports containing any problems encountered during shipping or receipt of specimens, summary statistics for each control pool, QC graphs, instrument calibration, reagents, and any special considerations are submitted to NCHS and Westat quarterly. The reports are reviewed for trends or shifts in the data. The laboratories are required to explain any identified areas of concern.

All QC procedures recommended by the manufacturers were followed. Reported results for all assays meet the Division of Laboratory Science’s quality control and quality assurance performance criteria for accuracy and precision (similar to specifications outlined by Westgard (1981)) (Caudil SP, et al. 2008).

Analytic Notes

Subsample weights

Serum polyfluorinated chemicals were measured in a one third subsample of persons 12 years and over. Special sample weights are required to analyze these data properly. Specific sample weights for this subsample are included in this data file and should be used when analyzing these data.

Variance estimation

The analysis of NHANES laboratory data must be conducted with the key survey design and basic demographic variables. The NHANES Demographic Data File contains demographic and sample design variables. The recommended procedure for variance estimation requires use of stratum and PSU variables (SDMVSTRA and SDMVPSU, respectively) in the demographic data file.

Links to NHANES Data Files

This laboratory data file can be linked to the other NHANES data files using the unique survey participant identifier SEQN.

Detection Limits

The detection limits were constant for all of the analytes in the data set. Two variables are provided for each of these analytes. The variable named LBD___LC indicates whether the result was below the limit of detection. There are two values: “0” and “1””. “0” means that the result was at or above the limit of detection. “1” indicates that the result was below the limit of detection.

The other variable named LBX___ provides the analytic result for that analyte.

The constant lower limits of detection for each polyfluorochemical is:

Analyte Item Id LLOD
Perfluorooctanoic acid LBXPFOA 0.1
Perfluorooctane sulfonic acid LBXPFOS 0.2
Perfluorohexane sulfonic acid LBXPFHS 0.1
2-(N-Ethyl-perfluorooctane sulfonamido) acetic acid LBXEPAH 0.1
2-(N-Methyl-perfluorooctane sulfonamido) acetic acid LBXMPAH 0.1
Pefluorodecanoic acid LBXPFDE 0.1
Perfluorobutane sulfonic acid LBXPFBS 0.1
Perfluoroheptanoic acid LBXPFHP 0.1
Perfluorononanoic acid LBXPFNA 0.082
Perfluorooctane sulfonamide LBXPFSA 0.1
Perfluoroundecanoic acid LBXPFUA 0.1
Perflurododecanoic acid LBXPFDO 0.1

Please refer to the Analytic Guidelines for further details on the use of sample weights and other analytic issues.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 12 YEARS - 150 YEARS

WTSC2YR - Two-year MEC weights of subsample C

Variable Name:
WTSC2YR
SAS Label:
Two-year MEC weights of subsample C
English Text:
Two-year MEC weights of subsample C
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 to 413068.05064 Range of Values 2432 2432
. Missing 0 2432

LBXEPAH - 2-(N-ethyl-PFOSA) acetate

Variable Name:
LBXEPAH
SAS Label:
2-(N-ethyl-PFOSA) acetate
English Text:
2-(N-Ethyl-perfluorooctane sulfonamido) acetic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.07 to 1 Range of Values 2233 2233
. Missing 199 2432

LBDEPAHL - 2-(N-ethyl-PFOSA) acetate comment

Variable Name:
LBDEPAHL
SAS Label:
2-(N-ethyl-PFOSA) acetate comment
English Text:
2-(N-Ethyl-perfluorooctane sulfonamido) acetic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 123 123
1 Below lower detection limit 2110 2233
. Missing 199 2432

LBXPFDE - Perfluorodecanoic acid

Variable Name:
LBXPFDE
SAS Label:
Perfluorodecanoic acid
English Text:
Pefluorodecanoic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.07 to 20.7 Range of Values 2233 2233
. Missing 199 2432

LBDPFDEL - Perfluorodecanoic acid comment

Variable Name:
LBDPFDEL
SAS Label:
Perfluorodecanoic acid comment
English Text:
Pefluorodecanoic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 2113 2113
1 Below lower detection limit 120 2233
. Missing 199 2432

LBXPFOA - Perfluorooctanoic acid

Variable Name:
LBXPFOA
SAS Label:
Perfluorooctanoic acid
English Text:
Perfluorooctanoic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.07 to 24 Range of Values 2233 2233
. Missing 199 2432

LBDPFOAL - Perfluorooctanoic acid comment

Variable Name:
LBDPFOAL
SAS Label:
Perfluorooctanoic acid comment
English Text:
Perfluorooctanoic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 2228 2228
1 Below lower detection limit 5 2233
. Missing 199 2432

LBXPFOS - Perfluorooctane sulfonic acid

Variable Name:
LBXPFOS
SAS Label:
Perfluorooctane sulfonic acid
English Text:
Perfluorooctane sulfonic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.14 to 281 Range of Values 2233 2233
. Missing 199 2432

LBDPFOSL - Perfluorooctane sulfonic acid comment

Variable Name:
LBDPFOSL
SAS Label:
Perfluorooctane sulfonic acid comment
English Text:
Perfluorooctane sulfonic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 2229 2229
1 Below lower detection limit 4 2233
. Missing 199 2432

LBXPFHS - Perfluorohexane sulfonic acid

Variable Name:
LBXPFHS
SAS Label:
Perfluorohexane sulfonic acid
English Text:
Perfluorohexane sulfonic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.07 to 44.8 Range of Values 2233 2233
. Missing 199 2432

LBDPFHSL - Perfluorohexane sulfonic acid comment

Variable Name:
LBDPFHSL
SAS Label:
Perfluorohexane sulfonic acid comment
English Text:
Perfluorohexane sulfonic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 2220 2220
1 Below lower detection limit 13 2233
. Missing 199 2432

LBXMPAH - 2-(N-methyl-PFOSA) acetate

Variable Name:
LBXMPAH
SAS Label:
2-(N-methyl-PFOSA) acetate
English Text:
2-(N-Methyl-perfluorooctane sulfonamido) acetic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.06 to 11.5 Range of Values 2233 2233
. Missing 199 2432

LBDMPAHL - 2-(N-methyl-PFOSA) acetate comment

Variable Name:
LBDMPAHL
SAS Label:
2-(N-methyl-PFOSA) acetate comment
English Text:
2-(N-Methyl-perfluorooctane sulfonamido) acetic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 1695 1695
1 Below lower detection limit 538 2233
. Missing 199 2432

LBXPFBS - Perfluorobutane sulfonic acid

Variable Name:
LBXPFBS
SAS Label:
Perfluorobutane sulfonic acid
English Text:
Perfluorobutane sulfonic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.07 to 1.4 Range of Values 2233 2233
. Missing 199 2432

LBDPFBSL - Perfluorobutane sulfonic acid comment

Variable Name:
LBDPFBSL
SAS Label:
Perfluorobutane sulfonic acid comment
English Text:
Perfluorobutane sulfonic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 19 19
1 Below lower detection limit 2214 2233
. Missing 199 2432

LBXPFHP - Perfluoroheptanoic acid

Variable Name:
LBXPFHP
SAS Label:
Perfluoroheptanoic acid
English Text:
Perfluoroheptanoic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.07 to 1 Range of Values 2233 2233
. Missing 199 2432

LBDPFHPL - Perfluoroheptanoic acid comment

Variable Name:
LBDPFHPL
SAS Label:
Perfluoroheptanoic acid comment
English Text:
Perfluoroheptanoic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 457 457
1 Below lower detection limit 1776 2233
. Missing 199 2432

LBXPFNA - Perfluorononanoic acid

Variable Name:
LBXPFNA
SAS Label:
Perfluorononanoic acid
English Text:
Perfluorononanoic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.058 to 19.352 Range of Values 2233 2233
. Missing 199 2432

LBDPFNAL - Perfluorononanoic acid comment

Variable Name:
LBDPFNAL
SAS Label:
Perfluorononanoic acid comment
English Text:
Perfluorononanoic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 2228 2228
1 Below lower detection limit 5 2233
. Missing 199 2432

LBXPFSA - Perfluorooctane sulfonamide

Variable Name:
LBXPFSA
SAS Label:
Perfluorooctane sulfonamide
English Text:
Perfluorooctane sulfonamide
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.07 to 0.2 Range of Values 2233 2233
. Missing 199 2432

LBDPFSAL - Perfluorooctane sulfonamide comment

Variable Name:
LBDPFSAL
SAS Label:
Perfluorooctane sulfonamide comment
English Text:
Perfluorooctane sulfonamide comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 3 3
1 Below lower detection limit 2230 2233
. Missing 199 2432

LBXPFUA - Perfluoroundecanoic acid

Variable Name:
LBXPFUA
SAS Label:
Perfluoroundecanoic acid
English Text:
Perfluoroundecanoic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.07 to 28.5 Range of Values 2233 2233
. Missing 199 2432

LBDPFUAL - Perfluoroundecanoic acid comment

Variable Name:
LBDPFUAL
SAS Label:
Perfluoroundecanoic acid comment
English Text:
Perfluoroundecanoic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 1555 1555
1 Below lower detection limit 678 2233
. Missing 199 2432

LBXPFDO - Perfluorododecanoic acid

Variable Name:
LBXPFDO
SAS Label:
Perfluorododecanoic acid
English Text:
Perflurododecanoic acid
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.07 to 2.8 Range of Values 2233 2233
. Missing 199 2432

LBDPFDOL - Perfluorododecanoic acid comment

Variable Name:
LBDPFDOL
SAS Label:
Perfluorododecanoic acid comment
English Text:
Perflurododecanoic acid comment code
Target:
Both males and females 12 YEARS - 150 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0 At or above the detection limit 113 113
1 Below lower detection limit 2120 2233
. Missing 199 2432