Table of Contents

Locator Record

Title: Epstein-Barr virus (VCA IgG) (SSEBV_E )
Contact Number: 1-866-441-NCHS
Years of Content: 2007 - 2008
First Published: June, 2012
Revised: NA
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Subject:Epstein-Barr virus VCA IgG antibodies measured by enzyme immunoassay
Record Source: NHANES 2007 - 2008
Survey Methodology: NHANES 2007 - 2008 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

Among children ages 6-19 years who participated in NHANES between 2007 and 2008 and had stored serum samples available, Epstein-Barr virus (EBV) antibody testing was conducted. EBV VCA IgG antibody was measured using a commercial enzyme immunoassay kit.

Eligible Sample

Surplus sera from children aged 6 to 19 years who participated in NHANES years 2007 to 2008.

Description of Laboratory Methodology

25 μL of sample is required for each assay. Specimens, calibrators and controls are pre-diluted 1:21 prior to placing them in the test wells. Cut-off calibrator, controls and subject samples are diluted according to the specific test kit protocol, mixed briefly by vortex to assure good distribution of the sample in the diluent, and then transferred to the appropriately labeled wells and incubated. After washing the wells, conjugate is added with another incubation and wash step following. Substrate is added and allowed to incubate before the addition of stop/color reagent. The plates are read at an absorbance of 450 nm using a reference wavelength of 630 nm. EIA indices are calculated by hand using the formulas in the kit package insert. Data are recorded (1) as Positive (EIA index ≥ 1.10), Negative (EIA index <0.90), or Equivocal (EIA index ≥ 0.90 to 1.09), and (2) as the exact numeric EIA index. The sensitivity of the assay is 96.6% and the specificity is 97.7%.

Data Processing and Editing

Data were submitted after all the antibody testing was complete. The data were not edited.

Laboratory Quality Assurance and Monitoring

Commercial reagents were used for the EBV VCA IgG EIA antibody testing. All QA/QC procedures recommended by the manufacturer were followed.

Analytic Notes

The EIA indices have 4 categorical interpretations.
1. Negative. These samples have an EIA index <0.90.
2. Positive. These samples have an EIA index ≥1.10.
3. Equivocal. These samples have an EIA index ≥ 0.90 to 1.099.*
4. QNS. Quantity insufficient for testing. These samples were assigned an arbitrary index of missing.

*The only way to be certain if subjects with equivocal EIA antibody indices have been infected with EBV is to obtain another specimen from them. Since this is not practical and since this group is only a small fraction of the subjects tested (~0.05%), the cleanest approach to analyzing the data is to exclude them.

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 6 YEARS - 19 YEARS

SSEBV - Epstein Barr Virus result

Variable Name:
SSEBV
SAS Label:
Epstein Barr Virus result
English Text:
Epstein Barr Virus result
Target:
Both males and females 6 YEARS - 19 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 1529 1529
2 Negative 516 2045
3 Equivocal 14 2059
4 Quantity not sufficient 1 2060
. Missing 0 2060

SSEBVIND - Epstein Barr Virus index

Variable Name:
SSEBVIND
SAS Label:
Epstein Barr Virus index
English Text:
Epstein Barr Virus index
Target:
Both males and females 6 YEARS - 19 YEARS
Code or Value Value Description Count Cumulative Skip to Item
0.01 to 7.17 Range of Values 2059 2059
. Missing 1 2060