Title: Phlebotomy Examination
Contact Number: 1-866-441-NCHS
Years of Content: 1999
First Published: June, 2002
Revised: December, 2005
Access Constraints: None
Use Constraints: None
Geographic Coverage: National
Record Source: NHANES 1999
Survey Methodology: NHANES 1999
is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files
The phlebotomy component of NHANES involved the collection of blood specimens. Prior to blood collection, the MEC phlebotomist administered a questionnaire to screen for conditions that would exclude participants from the blood draw. At this time, fasting status was determined. Blood specimens for basic hematology, nutritional biochemistry, and special studies was obtained Blood specimens are collected on participants aged one year and older in the MEC (or the respondent’s home when a home examination is performed).
Participants aged 1+ years of age
Protocol and Procedure
Data Collection Methods
The MEC phlebotomist draws blood from the participant’s arm. The volume of blood specimen for each age group is as follows:
- 1-2 years, 9 mL
- 3-5 years, 22 mL
- 6-11 years, 38 mL
- 12+ years 89-92 mL
Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM).
The NHANES 1999-2000 staff consisted of two phlebotomists. The American Society
for Clinical Pathologists or a similar organization certifies the phlebotomists. The MEC phlebotomists complete comprehensive training in pediatric phlebotomy techniques, including instruction by a pediatric nurse practitioner.
Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Each chapter in the LPM specifies the procedure to be used for preparation of the participant, specimen collection, labeling, processing, and preservation, and conditions for specimen transport that are appropriate for that method.
Data Processing and Editing
Phlebotomy data were recorded directly into a computerized database; all forms used in the MEC were automated.
Use PHAFSTHR and PHAFSTMN to determine the duration of fasting in hours and minutes, respectively.