Table of Contents

Locator Record

Title: Urinary Chlamydia and Gonorrhea (L05RDC_B )
Contact Number: 1-866-441-NCHS
Years of Content: 2001 - 2002
First Published: June, 2009
Revised: NA
Access Constraints: RDC Only
Use Constraints: RDC Only
Geographic Coverage: National
Subject:Urinary Chlamydia and Gonorrhea
Record Source: NHANES 2001 - 2002
Survey Methodology: NHANES 2001 - 2002 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files

Component Description

Urinary chlamydia and Urinary gonorrhea

Sexually transmitted infections caused by Chlamydia trachomatis and Neisseria gonorrhoeae may lead to pelvic inflammatory disease, ectopic pregnancy, infertility, and chronic pelvic pain in women. They are associated with increased risk of HIV transmission. Pregnant women may transmit infection to their newborn causing serious medical complications. At present there are no reliable estimates on the prevalence of chlamydial and gonococcal infection in the general population of the United States.

NHANES offers an opportunity to assess the prevalence of chlamydia and gonococcal infection in the general population and to monitor trends in prevalence as prevention programs are established and expanded.

Eligible Sample

Participants aged 14 to 39 years were tested. Public data file includes data for persons 18-39 years of age. Please see Analytic Notes for Data Users about the release of data for adolescents 14-17 years of age.

Description of Laboratory Methodology

Urinary chlamydia

The Chlamydia trachomatis assay uses LCR TM (ligase chain reaction) amplification technology in the LCx Probe System for the direct, qualitative detection of plasmid DNA of Chlamydia trachomatis.
The LCx Chlamydia trachomatis assay uses the nucleic acid amplication method LCR to detect the presence of C. trachomatis plasmid DNA directly in clinical specimens.

The four oligonucleotide probes in the LCx assay recognize and hybridize to a specific target sequence within the C. trachomatis plasmid DNA. The oligonuleotides are designed to be complementary to the target sequence so that in the presence of target, the probes will bind adjacent to one another. They can then be enzymatically joined to form the amplification product, which subsequently serves as an additional target sequence during further rounds of amplication. The product of the LCR reaction is detected on the Abbott LCx analyzer.

Urinary gonorrhea

The Neisseria gonorrhoeae assay uses LCR TM (ligase chain reaction) amplification technology in the LCx Probe System for the direct, qualitative detection of a specific target nucleic acid sequence in the Opa gene of Neisseria gonorrhoeae.

The LCx Neisseria gonorrhoeae assay uses the nucleic acid amplication method LCR to detect the presence of Neisseria gonorrhoeae. The four oligonucleotide probes in the LCx assay recognize and hybridize to a specific target sequence within the Opa gene of Neisseria gonorrhoeae DNA. The oligonuleotides are designed to be complementary to the target sequence so that in the presence of target, the probes will bind adjacent to one another. They can then be enzymatically joined to form the amplification product which subsequently serves as an additional target sequence during further rounds of amplication. The product of the LCR reaction is detected on the Abbott LCx analyzer.

Data Processing and Editing

Blood specimens were processed, stored and shipped to the Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, National Centers for Disease Control and Prevention for analysis. Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM).

Read the LABDOC file for detailed data processing and editing protocols. The analytical methods are described in the Description of the Laboratory Methodology section.

Laboratory Quality Assurance and Monitoring

The NHANES quality control and quality assurance protocols (QA/QC) meet the 1988 Clinical Laboratory Improvement Act mandates. Detailed quality control and quality assurance instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Read the LABDOC file for detailed QA/QC protocols.

Analytic Notes

 Public data file includes data for persons 20-39 years of age. Urinary chlamydia and gonorrhea data for youth 14-17 years of age will be in the Research Data Center (RDC) or through special agreement.

Collaborators may obtain the 2001-2002 NHANES Adolescent STD Special Use Datafile through a special agreement. The data set is a SAS file containing 3 variables for examined participants aged 14-17 years. Other interested researchers may use this file in the NCHS Research Data Center (RDC). The variable descriptors and variable names are as follows:

References

Codebook and Frequencies

SEQN - Respondent sequence number

Variable Name:
SEQN
SAS Label:
Respondent sequence number
English Text:
Respondent sequence number.
Target:
Both males and females 14 YEARS - 17 YEARS

URXUGC - Gonorrhea, urine

Variable Name:
URXUGC
SAS Label:
Gonorrhea, urine
English Text:
Gonorrhea, urine
Target:
Both males and females 14 YEARS - 17 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 5 5
2 Negative 1167 1172
3 Indeterminate 0 1172
. Missing 59 1231

URXUCL - Chlamydia, urine

Variable Name:
URXUCL
SAS Label:
Chlamydia, urine
English Text:
Chlamydia, urine
Target:
Both males and females 14 YEARS - 17 YEARS
Code or Value Value Description Count Cumulative Skip to Item
1 Positive 37 37
2 Negative 1135 1172
3 Indeterminate 0 1172
. Missing 59 1231