Title: Urinary Chlamydia and Gonorrhea, Ages 14-17
Contact Number: 1-866-441-NCHS
Years of Content: 2007 - 2008
First Published: September, 2009
Revised: November, 2010
Access Constraints: RDC Only
Use Constraints: RDC Only
Geographic Coverage: National
Subject:Urinary Chlamydia and Gonorrhea, Sexually transmitted infections.
Record Source: NHANES 2007 - 2008
Survey Methodology: NHANES 2007 - 2008 is a stratified multistage probability sample of the civilian non-institutionalized population of the U.S.
Medium: NHANES Web site; SAS transport files
Sexually transmitted infections caused by Chlamydia trachomatis may lead to pelvic inflammatory disease, ectopic pregnancy, infertility, and chronic pelvic pain in women. They are associated with increased risk of HIV transmission. Pregnant women may transmit infection to their newborn, causing serious medical complications.
NHANES offers an opportunity to assess the prevalence of chlamydia and gonococcal infection in the general population and to monitor trends in prevalence as prevention programs are established and expanded.
Participants aged 14-39 years were tested. Public data file includes data for persons 18-39 years of age. Please see Analytic Notes for Data Users about the release of data for adolescents 14-17 years of age.
Urine specimens were processed, stored and shipped to the Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, National Centers for Disease Control and Prevention for analysis. Detailed specimen collection and processing instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM).
The BDProbeTec CT Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DNA Assays are based on the simultaneous amplification and detection of target DNA, using amplification primers and a fluorescent labeled detector probe. The Strand Displacement Amplification (SDA) reagents are dried in two separate disposable microwell strips. The processed sample is added to the Priming Microwell, which contains the amplification primers, fluorescent labeled detector probe, and other reagents necessary for amplification. After incubation, the reaction mixture is transferred to the Amplification Microwell, which contains two enzymes (a DNA polymerase and a restriction endonuclease) necessary for SDA. The Amplification Microwells are sealed to prevent contamination and then incubated in a thermally controlled fluorescent reader which monitors each reaction for the generation of amplified products. The presence or absence of C. trachomatis and N. gonorrhoeae is determined by relating the BDProbeTec ET MOTA (Method Other Than Acceleration) scores for the sample to pre-determined cutoff values. The MOTA score is a metric used to assess the magnitude of signal generated as a result of the reaction.
There were no changes (from the previous 2 years of NHANES) to equipment, lab methods or lab site.
Read the General Documentation of Laboratory Data file for detailed data processing and editing protocols. The analytical methods are described in the Analytic Notes for Data Users section below.
Public data files include data for persons 18-39 years of age. Urinary Chlamydia data for youth 14-17 years of age will only be available in the Research Data Center (RDC) or through special agreement.
The NHANES quality assurance and quality control (QA/QC) protocols meet the 1988 Clinical Laboratory Improvement Act mandates. Detailed quality control and quality assurance instructions are discussed in the NHANES Laboratory/Medical Technologists Procedures Manual (LPM). Read the General Documentation of Laboratory Data file for detailed QA/QC protocols. A detailed description of the quality assurance and quality control procedures can be found on the NHANES website.
The analysis of NHANES laboratory data must be conducted with the key survey design and basic demographic variables. The NHANES Household Questionnaire Data Files contain demographic data, health indicators, and other related information collected during household interviews. They also contain all survey design variables and sample weights for these age groups. The phlebotomy file includes auxiliary information, such as the conditions precluding venipuncture. The household questionnaire and phlebotomy files may be linked to the laboratory data file using the unique survey participant identifier SEQN.
Exam sample weights should be used for analyses. Please refer to the Analytic Guidelines for further details on the use of sample weights and other analytic issues. The Analytic Guidelines are available on the NHANES website.
Collaborators may obtain the 2007-2008 NHANES Adolescent STD Special Use Data file through a special agreement. The data set is a SAS file containing 3 variables for examined participants aged 14-17 years. Other interested researchers may use this file in the NCHS Research Data Center (RDC). The variable descriptors and variable names are as follows:
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