Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 29, Number 5—May 2023
Dispatch

Unknown Circovirus in Immunosuppressed Patient with Hepatitis, France, 2022

Christophe RodriguezComments to Author , Laure Boizeau1, Alexandre Soulier1, Melissa N’Debi, Vanessa Demontant, Elisabeth Trawinski, Sarah Seng, Hélène Fontaine, Paul-Louis Woerther, Sarah Marchand, Slim Fourati, Stéphane Chevaliez, Pierre Cappy, Stanislas Pol, and Jean-Michel Pawlotsky
Author affiliations: Hôpital Henri Mondor, AP-HP, Université Paris-Est, Créteil, France (C. Rodriguez, L. Boizeau, A. Soulier, M. N’Debi, V. Demontant, E. Trawinski, S. Seng, P.-L. Woerther, S. Marchand, S. Fourati, S. Chevaliez, P. Cappy, J.-M. Pawlotsky); L’Institut Mondor de Recherche Biomédicale—INSERM U955, Créteil (C. Rodriguez, L. Boizeau, A. Soulier, S. Fourati, S. Chevaliez, P. Cappy, J.-M. Pawlotsky); Hôpital Cochin, AP-HP, Université Paris-Cité, Paris, France (H. Fontaine, S. Pol)

Main Article

Figure

Genomic and phylogenetic analysis of putative novel virus, Circovirus parisii, from an immunocompromised patient with hepatitis, France, 2022. A) Full-length genome of C. parisii reconstructed from shotgun metagenomics (SMg) sequence analysis. The genome is a 2021-nt single-stranded circular DNA containing 3 predicted open reading frames (ORFs), including ORF1 (replicase, green), ORF2 (capsid protein, blue) and ORF3 (red). The stem-loop contains an AGTATTAC sequence (origin of replication) that misses 1 nt (red dash) compared with other circoviruses. An ATG start codon is located at the 5′ end of the replicase gene. The replicase gene contains 6 conserved motifs, represented with a yellow background (amino acid and nucleotide sequences), with silent substitutions in red. B) Phylogenetic analysis of the replicase gene of the Circoviridae family, including the newly discovered C. parisii (red dot), known circoviruses (blue), and known cycloviruses (pink). Bootstrap values >70% are indicated with black stars. Scale bar indicates substitutions per site.

Figure. Genomic and phylogenetic analysis of putative novel virus, Circovirus parisii, from an immunocompromised patient with hepatitis, France, 2022. A) Full-length genome of C. parisii reconstructed from shotgun metagenomics (SMg) sequence analysis. The genome is a 2021-nt single-stranded circular DNA containing 3 predicted open reading frames (ORFs), including ORF1 (replicase, green), ORF2 (capsid protein, blue) and ORF3 (red). The stem-loop contains an AGTATTAC sequence (origin of replication) that misses 1 nt (red dash) compared with other circoviruses. An ATG start codon is located at the 5′ end of the replicase gene. The replicase gene contains 6 conserved motifs, represented with a yellow background (amino acid and nucleotide sequences), with silent substitutions in red. B) Phylogenetic analysis of the replicase gene of the Circoviridae family, including the newly discovered C. parisii (red dot), known circoviruses (blue), and known cycloviruses (pink). Bootstrap values >70% are indicated with black stars. Scale bar indicates substitutions per site.

Main Article

1These authors contributed equally to this article.

Page created: March 26, 2023
Page updated: April 19, 2023
Page reviewed: April 19, 2023
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external