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Volume 29, Number 12—December 2023
Research

Detection of Anopheles stephensi Mosquitoes by Molecular Surveillance, Kenya

Eric O. OchomoComments to Author , Sylvia Milanoi, Bernard Abong’o, Brenda Onyango, Margaret Muchoki, Diana Omoke, Evelyn Olanga, Laban Njoroge, Elijah Omondi Juma, James Dan Otieno, Damaris Matoke-Muhia, Luna Kamau, Cristina Rafferty, John E. Gimnig, Mildred Shieshia, Daniel Wacira, Joseph Mwangangi, Marta Maia, Charles Chege, Ahmeddin Omar, Martin K. Rono, Lucy Abel, Wendy Prudhomme O’Meara, Andrew Obala, Charles Mbogo, and Lenson Kariuki
Author affiliations: Kenya Medical Research Institute, Nairobi, Kenya (E.O. Ochomo, S. Milanoi, B. Abong’o, B. Onyango, M. Muchoki, D. Omoke, D. Matoke-Muhia, L. Kamau, J. Mwangangi, M. Maia, M.K. Rono, C. Mbogo); PMI Kinga Malaria Project, Abt Associates Inc., Kisumu, Kenya (E. Olanga); National Museums of Kenya, Nairobi (L. Njoroge); Pan-African Mosquito Control Association, Nairobi (E.O. Juma, D. Matoke-Muhia, C. Mbogo); World Health Organization, Kenya Country Office, Nairobi (J.D. Otieno); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C. Rafferty, J.E. Gimnig); US President’s Malaria Initiative, Washington, DC, USA (C. Rafferty, J.E. Gimnig, M. Shieshia, D. Wacira); University of Oxford, Oxford, UK (M. Maia); KEMRI-Wellcome Trust, Kilifi, Kenya (M. Maia, M.K. Rono); Division of National Malaria Program, Ministry of Health, Nairobi (C. Chege, A. Omar); Moi University, Eldoret (W.P. O’Meara, A. Obala); AMPATH, Eldoret, Kenya (L. Abel); Duke University, Durham, North Carolina, USA (W.P. O’Meara, L. Kariuki)

Main Article

Table 3

Results for sequencing analysis of 4 mosquito samples from Marsabit County, Kenya, sent to CDC for identification in study of Anopheles stephensi mosquito, Kenya

Origin sample ID CDC sample ID Morphologic ID Confirmed species ITS2 sequences
 CO1 sequences
GenBank accession no. GenBank accession no. of closest match % Identity match GenBank accession no. GenBank accession no. of closest match % Identity match
KE83GY 2023KEN0001 Suspected An. stephensi An. stephensi OQ275144 FJ526599.1 99.40 OR607949 OM801708 100
KE83QF 2023KEN0002 Suspected An. stephensi An. stephensi OQ275145 MW732931.1 100 OR607950 OM801708 99.70
KE83FZ 2023KEN0003 Suspected An. stephensi An. stephensi OQ275146 MW732931.1 100 OR607951 MN329060.1 99.10
KE831Q 2023KEN0004 Suspected An. stephensi Did not amplify NA NA NA NA

*CDC, US Centers for Disease Control and Prevention; CO1, cytochrome c oxidase subunit 1; ID, identification; ITS2, internal transcribed spacer 2.

Main Article

Page created: October 11, 2023
Page updated: November 18, 2023
Page reviewed: November 18, 2023
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