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Volume 29, Number 7—July 2023
Dispatch

Detecting, Quantifying, and Isolating Monkeypox Virus in Suspected Cases, Spain

Marta E. Álvarez Argüelles, Zulema Pérez Martínez, Susana Rojo Alba, Jose María González Alba, Ana María Fernandez-Verdugo, Isabel Costales González, Garbriel Martín Rodríguez, Jose Antonio Boga Riveiro, Mario Margolles Martins, and Santiago Melón GarcíaComments to Author 
Author affiliations: Hospital Universitario Central de Asturias, Oviedo, Spain (M.E. Álvarez Argüelles, Z. Pérez Martínez, S. Rojo Alba, J.M. González Alba, A.M. Fernandez-Verdugo, I. Costales González, G.M. Rodríguez, J.A. Boga Riveiro, S. Melón García); Epidemiological Surveillance Department, Oviedo (M. Margolles Martins)

Main Article

Table 2

Results of days of isolation and average cycle threshold values of samples according to results of culture in study of MPXV detection and isolation, Spain*

Cell No. positive cultures Mean days of positivity +SD (range) [95% CI] Mean Ct of positive cultures +SD (range) [95% CI] No. negative cultures Mean Ct of negative cultures +SD (range) [95% CI] p value
MRC-5 cells
12
5.8 +2.4 (3–10)† [4.2–7.4]
22.4 +6.4 (15–34) [18.3–26.5]
9
29.5 +3.1 (25–34) [27.1–31]
0.0068
VeroE6 cells
16
3.7 +1.3 (3–7)† [2.9–4.5]
23.4 +5.8 (15–34) [20.3–26.4]
5
32.02 +1.1 (31–34) [30.8–33.5]
0.0035
Both lines 17 3.7 +1.3 (3–7) [2.9–4.5] 23.9 +5.9 (15–34) [20.8–26.9] 4 32.25 +1.25 (31–34) [30.2–34.2] 0.0099

*Ct, cycle threshold; MPXV, monkeypox virus. †p = 0.012.

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Page created: May 29, 2023
Page updated: June 20, 2023
Page reviewed: June 20, 2023
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