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Volume 26, Number 9—September 2020
Research

Detection of H1 Swine Influenza A Virus Antibodies in Human Serum Samples by Age Group1

Elien Vandoorn, Isabel Leroux-Roels, Geert Leroux-Roels, Anna Parys, Amy Vincent, and Kristien Van ReethComments to Author 
Author affiliations: Ghent University, Merelbeke, Belgium (E. Vandoorn, A. Parys, K. Van Reeth); Ghent University and Ghent University Hospital, Ghent, Belgium (I. Leroux-Roels, G. Leroux-Roels); National Animal Disease Center, Ames, Iowa, USA (A. Vincent)

Main Article

Table 2

Percentage amino acid homology (lower left) and number of identical amino acids out of 50 aa in presumed antigenic sites (upper right) (28) between hemagglutinin 1 of human and swine H1 influenza A viruses used as test viruses in hemagglutination inhibition and virus neutralization assays*

Virus strain
 Eurasian avian
Human seasonal
Classical swine
swG10
 swHK11
 TW86†
 swG12
 NC99†
 swAL16
 swIL10
 swOK13
 BR07†
 swOH07
 CA09
Europe 1C.2.1
 Asia 1C.2.3
 1B.1-like
 Europe 1B.1.2.1
 1B.2-like
 N. Am. 1B.2.2.1
 N. Am. 1B.2.2.2
 N. Am. 1B.2.2.2
 1B.2-like
 N. Am. 1A.3.3.3
 World 1A.3.3.2
swG10 – 49 22 19 23 24 23 23 23 32 34
swHK11
 96.0
 –
23
 20
 23
 25
 23
 23
 23
33
 35
TW86 73.4 72.2 – 41 41 38 36 32 38 23 23
swG12
 69.1
 68.5
89.6
 –
 35
 33
 33
 32
 32
21
 22
NC99 73.3 72.1 93.9 85.9 – 42 42 36 47 24 24
swAL16 71.5 71.2 89.3 81.6 91.4 – 45 40 40 23 27
swIL10 72.1 71.5 89.6 83.4 94.2 92.9 – 43 42 23 25
swOK13
 72.0
 72.0
86.8
 81.8
 90.2
 89.2
 94.8
 –
 36
20
 24
BR07
 72.7
 71.5
90.8
 83.7
 96.9
 89.9
 92.3
 88.6
 –
24
 24
swOH07 74.0 74.3 74.3 71.6 73.0 72.4 71.8 70.5 73.6 – 44
CA09 73.4 72.5 73.4 70.9 72.4 73.9 72.1 71.1 72.4 90.8 –

*Viruses are ordered chronologically according to the year of circulation of the selected human test viruses TW86, NC99, BR07, and CA09; horizontal rules within table represent grouping of epidemiologically related human and swine influenza A viruses, with the oldest (ancestor) virus mentioned first. Complete isolate names are provided in Table 1. IAV, influenza A virus; N. Am., North America; –, not applicable.
†Human IAV that no longer circulates; TW86 is the presumed human precursor for human-like H1 swine IAVs in Europe; NC99 is the presumed human precursor for human-like H1 swine IAVs in North America; BR07 is a human seasonal IAV that circulated right before the influenza A(H1N1)pdm09 virus; the influenza A(H1N1)pdm09 virus, represented by CA09, is circulating in both humans and swine worldwide.

Main Article

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1Preliminary results from this study were presented at the Fourth International Symposium on Neglected Influenza Viruses, April 18–20, 2018, Brighton, United Kingdom; at the BELVIR conference, December 20, 2018, Brussels, Belgium; and at the 1918 Pandemic Conference, February 7–8, 2019, Ypres, Belgium.

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