Volume 21, Number 10—October 2015
Dispatch
Utility of Oral Swab Sampling for Ebola Virus Detection in Guinea Pig Model
Jessica R. Spengler, Ayan K. Chakrabarti, JoAnn D. Coleman-McCray, Brock E. Martin, Stuart T. Nichol, Christina F. Spiropoulou, and Brian H. Bird
Table
qRT-PCR results for EBOV nucleoprotein from guinea pig oral swab and blood samples collected, by number of days postinfection*
| ID | Virus type | Dose, TCID50/mL | Sample type, blood or oral/blood† |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D0 | D1 | D2 | D3 | D4 | D5 | D6 | D7 | D8 | D9 | D10 | D11 | D12 | D14 | |||
| 1 | GP-EBOV | 5.0 × 100 | – | – | – | –/– | – | – | + | + | ++ | +++/++++ | NS | NS | NS | NS |
| 2 | GP-EBOV | 5.0 × 100 | – | – | – | –/– | – | – | + | ++ | +++ | +++/+++++ | ++++ | ++++ | +++ | ++++ |
| 3 | GP-EBOV | 5.0 × 100 | – | – | – | –/– | – | – | + | +++ | ++++ | ++++/+++++ | +++++ | ++++ | +++ | ++++ |
| 4 | GP-EBOV | 5.0 × 100 | – | – | – | –/– | – | – | ++ | +++ | +++ | ++++/+++++ | ++++ | ++++ | +++ | ++++ |
| 5 | GP-EBOV | 5.0 × 100 | – | – | – | –/NS | – | – | – | ++ | +++ | ++++/+++++ | ++++ | ++++ | ++++ | ++++ |
| 6 | GP-EBOV | 5.0 × 103 | – | – | – | –/– | – | – | + | +++ | +++ | +++/++++ | +++ | +++ | ++ | +++ |
| 7 | GP-EBOV | 5.0 × 103 | – | – | – | –/+ | – | – | ++ | +++ | +++ | +++/+++++ | ++++ | ++++ | +++ | ++++ |
| 8 | GP-EBOV | 5.0 × 103 | – | – | – | –/NS | – | – | ++ | ++ | +++ | +++/++++ | ++++ | +++++ | +++ | ++++ |
| 9 | GP-EBOV | 5.0 × 103 | – | – | – | –/– | – | – | – | – | – | – | + | + | +/+++ | ++ |
| 10 | GP-EBOV | 5.0 × 103 | – | – | – | –/+++ | – | – | ++ | ++ | +++ | ++++/+++ | ++++ | +++++ | +++ | +++ |
| 11 | WT-Makona | 5.0 × 105 | – | – | – | –/– | – | – | – | – | – | – | – | – | – | – |
| 12 | WT-Makona | 5.0 × 105 | – | – | – | –/++ | – | – | – | – | – | – | – | – | – | – |
| 13 | WT-Makona | 5.0 × 105 | – | – | – | –/– | – | – | – | – | – | – | – | – | – | – |
| 14 | WT-Makona | 5.0 × 105 | – | – | – | –/++ | – | – | + | + | + | – | – | – | – | – |
| 15 | WT-Makona | 5.0 × 105 | – | – | – | –/+ | – | – | – | – | – | – | – | – | – | – |
| 16 | WT-Mayinga | 5.0 × 105 | – | – | – | –/++ | – | – | – | – | – | – | – | – | – | – |
| 17 | WT-Mayinga | 5.0 × 105 | – | – | – | –/– | – | – | + | – | – | – | – | – | – | – |
| 18 | WT-Mayinga | 5.0 × 105 | – | – | – | –/NS | – | + | – | + | + | – | – | – | – | – |
| 19 | WT-Mayinga | 5.0 × 105 | – | – | – | –/– | – | – | – | – | – | – | – | – | – | – |
| 20 | WT-Mayinga | 5.0 × 105 | – | – | – | –/+++ | – | – | – | ++ | + | +/+ | ++ | ++ | ++ | +++ |
| 21 | Neg control | DMEM | – | – | – | –/NS | – | – | – | – | – | – | – | – | – | – |
| 22 | Neg control | DMEM | – | – | – | –/– | – | – | – | – | – | – | – | – | – | – |
| 23 | Neg control | DMEM | – | – | – | –/– | – | – | – | – | – | – | – | – | – | – |
*Boldface indicates postmortem samples. Gray shading indicates period of overt clinical disease (days 6–9 postinfection). D, days postinfection; DMEM, Dulbecco's Modified Eagle's medium; EBOV, Ebola virus; GP, guinea pig–adapted; ID, identification number; Neg, negative; NS, not sampled; qRT-PCR, quantitative reverse transcription PCR; TCID50, 50% tissue culture infectious dose; WT, wild-type.
†EBOV RNA copies/mL: –, negative; +, 100–101; ++, 102; +++; 103; ++++, 104; +++++, 105–106.
Page created: September 22, 2015
Page updated: September 22, 2015
Page reviewed: September 22, 2015
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