Skip Navigation LinksSkip Navigation Links
Centers for Disease Control and Prevention
Safer Healthier People
Blue White
Blue White
bottom curve
CDC Home Search Health Topics A-Z spacer spacer
Blue curve MMWR spacer

Persons using assistive technology might not be able to fully access information in this file. For assistance, please send e-mail to: Type 508 Accommodation and the title of the report in the subject line of e-mail.

Notice to Readers: Use of Onsite Technologies for Rapidly Assessing Environmental Bacillus anthracis Contamination on Surfaces in Buildings

Environmental sampling to ascertain the presence of Bacillus anthracis spores in buildings is an important tool for assessing risk for exposure. Similar to diagnostic testing, culture with positive identification of B. anthracis (CDC culture method) is the confirmatory test. Laboratory-based polymerase chain reaction (PCR) methods for detecting genetic material of B. anthracis can be used in preliminary assessments and as adjuncts to microbiologic methods. Although these tests are consistent with culture results, PCR methods are not approved by the Food and Drug Administration, and results should not be the basis for clinical decisions.

Rapid-assay devices that can provide results within minutes are used for onsite detection of environmental contamination. Some of these devices are PCR-based assays, and others are immune-based assays for B. anthracis. CDC has not obtained validation data for rapid-assay devices. A recent CDC evaluation of B. anthracis contamination at the Brentwood postal facility in the District of Columbia included use of one onsite PCR-based device and CDC culture method. Of 107 samples analyzed using CDC culture method and the PCR-based device, 95 (89%) were negative by both methods. Of six samples identified as positive by CDC culture method, two were positive using the PCR-based device. Of eight samples identified as positive by the PCR-based device, two were positive by CDC culture method. Although these results indicate a poor agreement between results from the onsite PCR-based device and CDC culture method, this assessment was not intended as a formal validation test because of limited capacity to implement adequate quality-control measures and the small number of B. anthracis positive samples.

The apparently poor agreement of the onsite PCR-based device could be attributed to several factors such as the concentration of spores on contaminated surfaces, sample collection and preparation procedures, sample splitting, and the methods used for removing the sample from collection material. Furthermore, PCR- or immune-based tests do not distinguish viable from nonviable spores and can produce positive scores for samples that culture methods would define as negative. As a result, these methods are not useful for evaluating the success of disinfection techniques that do not remove nonviable spores.

Public health officials are urged to understand the limitations of onsite, rapid technologies for B. anthracis before using them for public health decision making. Until validation testing is complete and guidelines for effective use are developed, PCR- or immune-based assay results for B. anthracis should not be used alone, but should be confirmed with samples analyzed by culture methods to make public health decisions.

Use of trade names and commercial sources is for identification only and does not imply endorsement by the U.S. Department of Health and Human Services.

References to non-CDC sites on the Internet are provided as a service to MMWR readers and do not constitute or imply endorsement of these organizations or their programs by CDC or the U.S. Department of Health and Human Services. CDC is not responsible for the content of pages found at these sites.

Disclaimer   All MMWR HTML versions of articles are electronic conversions from ASCII text into HTML. This conversion may have resulted in character translation or format errors in the HTML version. Users should not rely on this HTML document, but are referred to the electronic PDF version and/or the original MMWR paper copy for the official text, figures, and tables. An original paper copy of this issue can be obtained from the Superintendent of Documents, U.S. Government Printing Office (GPO), Washington, DC 20402-9371; telephone: (202) 512-1800. Contact GPO for current prices.

**Questions or messages regarding errors in formatting should be addressed to

Page converted: 12/6/2001


Safer, Healthier People

Morbidity and Mortality Weekly Report
Centers for Disease Control and Prevention
1600 Clifton Rd, MailStop E-90, Atlanta, GA 30333, U.S.A


Department of Health
and Human Services

This page last reviewed 12/6/2001