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Persons using assistive technology might not be able to fully access information in this file. For assistance, please send e-mail to: mmwrq@cdc.gov. Type 508 Accommodation and the title of the report in the subject line of e-mail. Notice to Readers: Update: West Nile Virus Isolated from Mosquitoes --- New York, 2000During January--February 2000, three pools of overwintering Culex spp. mosquitoes collected at Fort Totten, New York, were positive for West Nile Virus (WNV) RNA by real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay (TaqMan, Perkin-Elmer Biosystems, Foster City, California*) (1). Additional testing to detect the presence of intact virus was performed but was negative as of March 10, 2000. Since then, one of the pools that was positive by RT-PCR yielded a live WNV isolate in a fluid vero cell culture system and confirmed by West Nile-specific monoclonal antibody staining of virus infected cells and virus gene sequencing. Studies of this isolate continue. Reference
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