Skip directly to search Skip directly to A to Z list Skip directly to site content
CDC Home

Global Progress Toward Laboratory Containment of Wild Polioviruses --- July 2001--August 2002

Since the World Health Assembly launched the Global Poliomyelitis Eradication Initiative in 1988 (see box), the number of countries in which wild poliovirus is endemic has decreased from 125 to 10 in 2001. Three of the six World Health Organization (WHO) regions (Americas, European, and Western Pacific) have been certified as free of wild poliovirus transmission (1--4). The Global Commission for the Certification of the Eradication of Poliomyelitis will declare the world polio-free when all regions have documented the absence of wild poliovirus transmission for at least 3 consecutive years and when laboratories with wild poliovirus--containing materials have implemented appropriate containment conditions (5). This report describes preparations for laboratory containment and the creation of a global inventory of laboratories and institutions retaining wild poliovirus and summarizes global progress since July 2001 (6). The data indicate that substantial progress has been made in identifying laboratories with wild poliovirus--containing materials and in conducting national wild poliovirus inventories.

In 1999, the World Health Assembly recommended that all member states "begin the process leading to laboratory containment of wild poliovirus" (7). As of August 2002, a total of 138 (64%) of 214 countries and areas had appointed national task forces for laboratory containment activities, compared with 110 (51%) in June 2001 (6); 121 (57%) countries and areas were conducting surveys of laboratories, and 76 (36%) had completed surveys and submitted national inventories to regional certification commissions (Figure), compared with 11 (5%) in June 2001. These inventories have identified 1,242 laboratories with wild poliovirus materials (Table).

Laboratory containment activities are of the highest priority in those regions that have been certified as free of wild poliovirus transmission. In the Americas, laboratory surveys are ongoing in 14 (29%) of the region's 48 countries. Canada completed a survey of approximately 1,700 institutions in 2001 and is following up with 22 (1%) laboratories that reported holding wild poliovirus--containing materials. In 2002, the United States completed a pilot survey of 306 institutions with 2,951 laboratories, 47 (2%) of which reported retaining wild poliovirus--containing materials; in October 2002, a nationwide survey began of 30,097 clinics, 450 academic institutions, 637 biomedical institutions, 56 state and local health departments, and 12 federal government departments. Completion of the inventory is anticipated in mid-2003.

In 2001, containment activities in the European Region were accelerated in anticipation of the region being certified polio-free in June 2002 (4). Each of the region's 51 countries has established a national task force, created a plan of action, compiled a list of laboratories, and initiated a national survey, and 41 (80%) countries have submitted national inventories to the European Regional Certification Commission. The 10 (20%) countries that have not yet submitted inventories are highly industrialized Western European nations that face substantial logistical challenges in contacting a large number of biomedical institutions.

In 2001, Germany enacted legislation requiring laboratories with wild poliovirus materials to comply with the survey and with recommended biosafety procedures. Approximately 3,500 institutions were identified and surveyed; the response rate was 100%. The contents of approximately 7,000 deep freezers were reviewed. Wild poliovirus--containing materials were reported in 54 (2%) laboratories, 26 (48%) of them in academic institutions; 30 (56%) laboratories destroyed the materials, and 24 (44%) retained them under the required biosafety conditions.

In the Western Pacific, the first WHO region to begin containment activities, 31 (86%) of 36 countries have submitted national inventories; 69 of 13,178 surveyed laboratories reported stocks of materials containing wild poliovirus. Of the five countries with surveys still in progress, the three countries (Australia, China, and Japan) with the largest numbers of laboratories in the region face logistical challenges similar to those facing countries in Western Europe and North America. The other two countries (the Philippines and Malaysia) also face challenges in identifying correct contact information for many unregistered laboratories.

Laboratory containment activities also are under way in the three regions (African, Eastern Mediterranean, and South East Asian) that have not yet been certified as polio-free. Countries in regions that have not reported polio cases in several years have been encouraged to begin containment activities. Seven African countries have established national task forces, with Cameroon and Uganda serving as pilot countries, and 17 Eastern Mediterranean countries and five South East Asian countries have initiated surveys. Four countries in the Eastern Mediterranean Region have submitted national inventories to the Eastern Mediterranean Region Certification Commission.

Reported by: Vaccines and Biologicals Dept, World Health Organization, Geneva, Switzerland. Div of Viral and Rickettsial Diseases, National Center for Infectious Diseases; Global Immunization Div, National Immunization Program, CDC.

Editorial Note:

Considerable progress has been made toward completing the global inventory of laboratories and institutions retaining wild poliovirus--containing materials. Countries in all six WHO Regions are implementing laboratory containment activities, and the WHO Global Action Plan for Laboratory Containment has been revised to incorporate the lessons learned from these experiences (8). The experience in Germany illustrates the challenges countries with a long history of biomedical research and decentralized health structures face in compiling inventories. The action plan recommends that the number of laboratories with wild poliovirus--containing materials be decreased but allows such materials to be retained by laboratories listed on the national inventory that meet prescribed biosafety conditions, including having basic biosafety level (BSL-2) facilities and practices, limited laboratory access, polio vaccination of personnel, and accurate records of poliovirus materials.

When global wild poliovirus transmission is interrupted, laboratories will be notified that high-containment laboratory (BSL-3/polio) measures are required for all laboratory activities involving known wild poliovirus--containing materials. The same measures are required for all activities involving poliovirus replication in permissive cells or animals using potential wild poliovirus--infectious materials (e.g., fecal, respiratory, and environmental samples collected for any purpose when and where wild poliovirus was known or suspected to be present). For all other activities with potential wild poliovirus--infectious materials, the requirements remain unchanged. Bacteriology and parasitology laboratories may continue to work with potential wild poliovirus--containing materials under BSL-2/polio conditions, which include the use of standard class II biological safety cabinets.

These biosafety recommendations are anticipated to remain in effect as long as current global polio vaccination policies continue. However, the plan recognizes that the consequences of a reintroduction of wild poliovirus from a laboratory will increase after polio vaccination is stopped within a country or region. Containment requirements under this scenario will be reexamined and increased for wild poliovirus and oral poliovirus vaccine materials.

Laboratory containment of wild poliovirus--containing materials is an essential component for the eradication of wild poliovirus. Countries are cooperating successfully to implement laboratory containment activities, and the goal of identifying laboratories with wild poliovirus materials is being achieved. All countries in which polio is not endemic are anticipated to complete a national inventory of laboratories holding wild poliovirus--containing materials by the end of 2003.

References

  1. CDC. Progress towards global eradication of poliomyelitis, 2001. MMWR 2002;51:253--6.
  2. CDC. Certification of poliomyelitis eradication---the Americas, 1994. MMWR 1994;43:720--2.
  3. CDC. Certification of poliomyelitis eradication---Western Pacific Region, October 2000. MMWR 2001;50:1--3.
  4. CDC. Certification of poliomyelitis eradication---European Region, June 2002. MMWR 2002;51:572--4.
  5. Department of Vaccines and Biologicals. Report of the third meeting of the Global Commission for the Certification of the Eradication of Polio, July 9, 1998. Geneva, Switzerland: World Health Organization, 1999.
  6. CDC. Global progress toward laboratory containment of wild polioviruses, June 2001. MMWR 2001;50:620--3.
  7. World Health Assembly. Poliomyelitis Eradication. Resolutions of the 52nd World Health Assembly. Geneva, Switzerland: World Health Organization, 1999.
  8. World Health Organization. WHO global action plan for laboratory containment of wild polioviruses (draft). Geneva, Switzerland: World Health Organization. Available at http://www.who.int/vaccines-polio/all/news/files/pdf/globalactionplan_2nd.pdf.


Table

Table 1
Return to top.
Figure

Figure 1
Return to top.
Box

Box
Return to top.



Use of trade names and commercial sources is for identification only and does not imply endorsement by the U.S. Department of Health and Human Services.

References to non-CDC sites on the Internet are provided as a service to MMWR readers and do not constitute or imply endorsement of these organizations or their programs by CDC or the U.S. Department of Health and Human Services. CDC is not responsible for the content of pages found at these sites. URL addresses listed in MMWR were current as of the date of publication.


All MMWR HTML versions of articles are electronic conversions from typeset documents. This conversion might result in character translation or format errors in the HTML version. Users are referred to the electronic PDF version (http://www.cdc.gov/mmwr) and/or the original MMWR paper copy for printable versions of official text, figures, and tables. An original paper copy of this issue can be obtained from the Superintendent of Documents, U.S. Government Printing Office (GPO), Washington, DC 20402-9371; telephone: (202) 512-1800. Contact GPO for current prices.

**Questions or messages regarding errors in formatting should be addressed to mmwrq@cdc.gov.

 
USA.gov: The U.S. Government's Official Web PortalDepartment of Health and Human Services
Centers for Disease Control and Prevention   1600 Clifton Rd. Atlanta, GA 30333, USA
800-CDC-INFO (800-232-4636) TTY: (888) 232-6348 - Contact CDC–INFO
A-Z Index
  1. A
  2. B
  3. C
  4. D
  5. E
  6. F
  7. G
  8. H
  9. I
  10. J
  11. K
  12. L
  13. M
  14. N
  15. O
  16. P
  17. Q
  18. R
  19. S
  20. T
  21. U
  22. V
  23. W
  24. X
  25. Y
  26. Z
  27. #