N. polysaccharea was described in 1983 and is characterized by its ability to produce acid from glucose and maltose and polysaccharide from sucrose. Strains of this species were previously identified as nontypable strains of N. meningitidis. Strains of N. polysaccharea also may have been misidentified previously as N. subflava because their ability to produce polysaccharide from sucrose was not determined.
|Enzyme Substrate Test||Prolyl aminopeptidase +ve|
|Nitrate Reduction Test||Nitrate -ve|
|Polysaccharide from Sucrose|
(Reaction with 30% hydrogen peroxide)
Neisseria species which may be misidentified as N. polysaccharea by acid production tests and supplemental tests that differentiate between them
Table 2. Differential characteristics of Neisseria spp. which produce acid from glucose and maltose and supplemental tests that differentiate between them.
|N. polysaccharea||+||+||-||-||-||+||-||PAP +ve||(R)|
|N. meningitidis||-||-||GGT +ve||R|
|+||+||-||-||-||-||+||GGT +ve or PAP +ve||(R)|
|+||+||-||+||-||-||+||GGT +ve or PAP +ve||(R)|
Abbreviations: G, glucose; M, maltose, S, sucrose; F, fructose; L, lactose; PAP. Prolyl aminopeptidase; GGT,?(gamma)-glutamyltransferase; ß(beta)-GAL, ß(beta)-galactosidase, . +, most strains positive; -, most strains negative; (+), some strains give weak positive reactions which are not representative of the species; R, strains grow well on selective medium for N. gonorrhoeae and/or show no inhibition around a colistin disk (10 micrograms); (R), most strains susceptible, some strains resistant.
*The author has encountered one strain of lactose-negative N. lactamica; this strain was identified with an enzyme substrate test which demonstrated that the organism produced ß(beta)-galactosidase.
**N. subflava biovar flava will be identified as N. subflava biovar subflava if acid production from fructose is not determined.
Although enzyme substrate tests are intended to be used only for the identification of Neisseria spp. isolated on selective media for N. gonorrhoeae, isolates of N. polysaccharea may give the same reaction (PAP +ve) in this test as do isolates of N. gonorrhoeae. Thus additional tests must be performed to differentiate between these species.
Table 3. Supplemental tests which permit differentiation among Neisseria and related species that produce prolyl aminopeptidase in enzyme substrate tests
|Species that Produce|
|N. polysaccharea||Diplococci||+||+||-||-||-||-||+||Weak (1+) to|
strong (3+) positive
|N. flavescens||Diplococci||-||-||-||-||-||-||+||Weak (2+)|
|N. cinerea||Diplococci||(-)||-||-||-||-||-||-||Weak (2+)|
|N. gonorrhoeae||Diplococci||+||-||-||-||-||-||-||Strong (4+)|
|K. denitrificans||Rod filaments||+||-||-||-||-||+||-||-||R|
|N. sicca||Diplococci||+||+||+||+||-||-||+||Weak (2+)|
|N. mucosa||Diplococci||+||+||+||+||-||+||+||Weak (2+)|
|N. elongata||Rod filaments||-||-||-||-||-||-||-||-||S|
Abbreviations: G, glucose; M, maltose, S, sucrose; F, fructose; L, lactose; PAP. Prolyl aminopeptidase; GGT,γ(gamma)-glutamyltransferase; ß(beta)-GAL, ß(beta)-galactosidase. +, most strains positive; -, most strains negative; (+), some strains give weak positive reactions which are not representative of the species; R, strains grow well on selective medium for N. gonorrhoeae and/or show no inhibition around a colistin disk (10 micrograms); (R), most strains susceptible, some strains resistant.
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