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Because of differences in the bacteria that PulseNet subtypes, PulseNet has developed separate protocols for each bacteria that are subtyped by PFGE, MLVA, and WGS. Additionally, because of the variation between machines, MLVA protocols have been developed separately for specific machines. These protocols are listed below and can also be found on CDC’s Web pages describing each pathogen.

PFGE Protocols

MLVA Protocols

WGS Protocols

*Clostridium botulinum is considered a select agent and, as such, follows different handling procedures than other pathogens covered by PulseNet.